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发表日期： 1993 Sep 1
英文标题： Oxidants, antioxidants, and the degenerative diseases of aging.[全文]
中文标题： 氧化剂，抗氧化剂和衰老的退行性疾病。
论文作者： Ames, B N; Shigenaga, M K; Hagen, T M
英文摘要： Metabolism, like other aspects of life, involves tradeoffs. Oxidant by-products of normal metabolism cause extensive damage to DNA, protein, and lipid. We argue that this damage (the same as that produced by radiation) is a major contributor to aging and to degenerative diseases of aging such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts. Antioxidant defenses against this damage include ascorbate, tocopherol, and carotenoids. Dietary fruits and vegetables are the principal source of ascorbate and carotenoids and are one source of tocopherol. Low dietary intake of fruits and vegetables doubles the risk of most types of cancer as compared to high intake and also markedly increases the risk of heart disease and cataracts. Since only 9% of Americans eat the recommended five servings of fruits and vegetables per day, the opportunity for improving health by improving diet is great.Images: Fig. 1
中文摘要： 与生命的其他方面一样，新陈代谢也涉及权衡。正常代谢的氧化剂副产物会对DNA，蛋白质和脂质造成广泛的损害。我们认为，这种损害 (与辐射产生的损害相同) 是衰老和衰老退行性疾病的主要原因，例如癌症，心血管疾病，免疫系统衰退，脑功能障碍和白内障。针对这种损害的抗氧化剂防御包括抗坏血酸盐、生育酚和类胡萝卜素。膳食水果和蔬菜是抗坏血酸盐和类胡萝卜素的主要来源，并且是生育酚的一个来源。与高摄入量相比，低饮食摄入水果和蔬菜会使大多数类型癌症的风险增加一倍，并且还显着增加患心脏病和白内障的风险。因为只有9% 的美国人每天吃五份推荐的水果和蔬菜，通过改善饮食来改善健康的机会是很大的。图片: 图1
发表日期： 1993 Sep
中文标题： 消化不良患者的血清胃蛋白酶原I和II浓度以及针对幽门螺杆菌的IgG抗体。
论文作者： Biasco, G; Paganelli, G M; Vaira, D; Holton, J; Di Febo, G; Brillanti, S; Miglioli, M; Barbara, L; Samloff, I M
英文摘要： AIMS--To investigate the association between histologically confirmed gastritis, carriage of Helicobacter pylori and pepsinogen (PG) I and PG II concentrations. METHODS--Prospective study of 81 dyspeptic patients undergoing upper gastrointestinal endoscopy was made. The extent of gastric mucosal inflammation and the presence of H pylori was determined, and serology to evaluate PG I and II concentrations and IgG titres to H pylori was carried out. RESULTS--The presence of H pylori was strongly correlated with high IgG antibody titres to H pylori and gastritis. Patients who were H pylori positive had significantly higher PG I and PG II concentrations and a significantly lower PG I:PG II ratio than patients who were negative for H pylori. In 13 patients with duodenal ulcer and H pylori positive gastritis serum PG I concentrations were significantly higher than in H pylori positive patients without duodenal ulcer. Significant correlations were found between the age of patients and serum PG II, the PG I:PG II ratio, IgG antibodies to H pylori, the severity of body gastritis and H pylori infection, and between the degree of gastritis in the body of the stomach and the PG II concentration. CONCLUSIONS--Serum PG I and II concentrations, together with a fall in the PG I:PG II ratio, could be used as predictors of H pylori infection as well as serum IgG antibody response to H pylori.
中文摘要： 目的-研究经组织学证实的胃炎，幽门螺杆菌携带与胃蛋白酶原 (PG) I和PG II浓度之间的关系。方法 -- 对81例行上消化道内镜检查的消化不良患者进行前瞻性研究。确定胃粘膜炎症的程度和幽门螺杆菌的存在，并进行血清学以评估PG I和II浓度以及对幽门螺杆菌的IgG滴度。结果-幽门螺杆菌的存在与幽门螺杆菌的高IgG抗体滴度和胃炎密切相关。与幽门螺杆菌阴性的患者相比，幽门螺杆菌阳性的患者的PG I和PG II浓度显着较高，PG I:PG II比率显着较低。在13例十二指肠溃疡和H pylori阳性胃炎患者中，血清PG I浓度明显高于无十二指肠溃疡的H pylori阳性患者。发现患者年龄与血清PG II，PG I:PG II比率，针对幽门螺杆菌的IgG抗体，身体胃炎和幽门螺杆菌感染的严重程度之间以及胃体胃炎的程度之间存在显着相关性。和PG II浓度。结论-血清PG I和II浓度以及PG I:PG II比率的下降可以用作幽门螺杆菌感染以及对幽门螺杆菌的血清IgG抗体反应的预测指标。
发表日期： 1993 Sep
中文标题： 胃窦和身体粘膜幽门螺杆菌胃炎中淋巴滤泡和聚集物的患病率。
论文作者： Eidt, S; Stolte, M
英文摘要： AIMS--To evaluate the prevalence of lymphoid follicles and aggregates in the antral and body mucosa in Helicobacter pylori gastritis and to assess if there were correlations with ulcers in the duodenum, pylorus, or stomach, and with chronic antral erosions. METHODS--Patients (n = 2692) with histologically confirmed H pylori antral gastritis were investigated. These comprised five groups: those with duodenal ulcers; those with pyloric ulcers; those with gastric ulcers; those with chronic erosions; and those with no associated lesions. In 1446 cases at least two additional biopsy specimens from the oxyntic mucosa were available. RESULTS--Lymphoid follicles and aggregates were found in 53.8% of cases in the antral mucosa compared with 14.8% in the oxyntic mucosa (p < 0.001). The various diseases showed significant differences in terms of the prevalence of follicles and aggregates: The highest numbers in the antral mucosa as well as the lowest in the oxyntic mucosa were found in patients with duodenal ulcers (60.5% and 9.2%, respectively). The highest numbers of follicles and aggregates in the oxyntic mucosa occurred in patients with gastric ulcers. CONCLUSIONS--The detection of lymphoid follicles and aggregates in oxyntic mucosa and the higher prevalence in antral mucosa fits well with the distribution of primary gastric lymphomas. This adds further weight to the notion that the development of follicles and aggregates, triggered by H pylori, might be an early precursor to gastric lymphoma. The differences between the groups investigated might be due to different strains of H pylori or differences in the respective sizes of antral and oxyntic mucosa.Images:
中文摘要： 目的-评估幽门螺杆菌胃炎中胃窦和身体粘膜中淋巴滤泡和聚集物的患病率，并评估是否与十二指肠，幽门或胃溃疡以及慢性胃窦糜烂有关。方法-对经组织学证实的幽门螺杆菌胃窦炎患者 (n = 2692) 进行调查。这些分为五组: 十二指肠溃疡; 那些患有幽门溃疡的人; 那些患有胃溃疡的人; 那些患有慢性糜烂的人; 以及那些没有相关病变的人。在1446情况下，至少有两个来自氧化粘膜的活检标本可用。结果-在胃窦粘膜中发现53.8% 例淋巴滤泡和聚集物，而在胃窦粘膜中发现14.8% 例 (p <0.001)。各种疾病在卵泡和聚集物的患病率方面显示出显着差异: 十二指肠溃疡患者的窦粘膜中数量最多，而氧化粘膜中数量最少 (分别为60.5% 和9.2%)。胃溃疡患者的氧化粘膜中卵泡和聚集物数量最多。结论-在氧化粘膜中检测到淋巴滤泡和聚集物以及在窦粘膜中较高的患病率与原发性胃淋巴瘤的分布非常吻合。这进一步加重了由幽门螺旋杆菌引发的卵泡和聚集物的发育可能是胃淋巴瘤的早期前体的观点。调查的各组之间的差异可能是由于不同的幽门螺杆菌菌株或胃窦和胃粘膜各自大小的差异。
发表日期： 1993 Sep
中文标题： 幽门螺杆菌胃炎患儿胃黏膜中的前列腺素E2: 与粘液凝胶层厚度的关系。
论文作者： Oderda, G; D'Alessandro, M; Mariani, P; Lionetti, P; Bonamico, M; Dell'Olio, D; Ansaldi, N
英文摘要： AIMS--To evaluate the changes in mucus gel layer thickness and prostaglandin E2 (PGE2) content caused by Helicobacter pylori infection in the antral mucosa of children: to assess whether decreased mucus gel thickness is related to PGE2 production. METHODS--Antral biopsy specimens were taken at endoscopy from 153 children. H pylori gastritis was evident in 45 and normal mucosa in 59. The other 49 children were studied one month after antibiotic treatment that eradicated the infection in 37 of them had been stopped. One antral specimen was immersed in ice-cold saline, put under an inverse microscope with an eyepiece graticule. Mucus gel thickness was measured and then the processed for histological examination; another specimen was weighed and processed for in vitro prostanoid generation. RESULTS--Mucus gel layer thickness was significantly decreased in children with H pylori gastritis (90 (SD) 29) microns v 120 (58) microns in controls, p < 0.01) but returned to control values after H pylori had been eradicated. PGE2 generation was significantly increased in children with H pylori gastritis (1022 (811) ng/g v 641 (473) ng/g in controls, p < 0.01). One month after treatment PGE2 generation significantly decreased in children without infection (880 (534), p < 0.01), but was still high where infection persisted. A significant inverse correlation was found between PGE2 generation and mucus gel layer thickness (p < 0.05). CONCLUSIONS--These data suggest that H pylori damages the mucus gel layer, and that the gastric mucosa increases generation of PGE2 in response to back diffusion of acid and pepsin.Images:
中文摘要： 目的 -- 评估幽门螺杆菌感染引起的儿童胃窦粘膜粘液凝胶层厚度和前列腺素E2 (PGE2) 含量的变化: 评估粘液凝胶厚度的降低是否与PGE2的产生有关。方法-内窥镜检查时从153名儿童中采集了肛门活检标本。幽门螺杆菌胃炎在45例中明显，正常粘膜在59例中明显。在停止抗生素治疗一个月后，对其他49名儿童进行了研究，该抗生素治疗消除了其中37名儿童的感染。将一个胃窦标本浸入冰冷的盐水中，放在带有目镜的倒置显微镜下。测量粘液凝胶厚度，然后进行处理以进行组织学检查; 称重另一样品并进行处理以用于体外前列腺素类产生。结果-幽门螺杆菌胃炎患儿的粘液凝胶层厚度显着降低 (对照组为90 (SD) 29) 微米v 120 (58) 微米，p <0.01)，但根除幽门螺杆菌后恢复到对照值。幽门螺杆菌胃炎患儿的PGE2生成显着增加 (对照组为1022 (811) ng/g v 641 (473) ng/g，p <0.01)。治疗后一个月，无感染儿童的PGE2生成显着下降 (880 (534)，p <0.01)，但在感染持续的情况下仍然很高。PGE2的产生与粘液凝胶层厚度之间存在显着的负相关 (p <0.05)。结论-这些数据表明，幽门螺杆菌会破坏粘液凝胶层，并且胃粘膜会响应酸和胃蛋白酶的反扩散而增加PGE2的产生。图像:
发表日期： 1993 Sep
中文标题： 应用幽门螺杆菌EIA-G和EIA-A检测血清免疫球蛋白G (IgG) 和IgA抗体诊断幽门螺杆菌感染。
论文作者： Russell, R I
英文摘要：
中文摘要：
发表日期： 1993 Sep
中文标题： 在存在或不存在非甾体类抗炎药的情况下，十二指肠组织学，溃疡和幽门螺杆菌。
论文作者： Taha, A S; Dahill, S; Nakshabendi, I; Lee, F D; Sturrock, R D; Russell, R I
英文摘要： Duodenitis and gastric metaplasia, which is often colonised by Helicobacter pylori (H pylori), are increasingly recognised for their importance in the pathogenesis of duodenal ulcers. The situation is not clear in patients receiving non-steroidal anti-inflammatory drugs (NSAIDs), who have a higher risk of peptic ulceration. The aim of this study was to identify the duodenal histological abnormalities in the presence or absence of NSAIDs, H pylori, and duodenal ulceration. Endoscopic duodenal biopsy specimens were taken from healthy looking mucosa of 172 patients (74 took NSAIDs, and 98 did not). Duodenitis was graded according to the degree of neutrophilic and plasma cell infiltration, villus height, Brunner's gland prolapse, and gastric metaplasia. The activity of duodenitis was dependent on the neutrophilic infiltration. A global score covering all the above factors was constructed, and H pylori in both the stomach and duodenum, was also assessed. The results showed that duodenitis with varying degrees of neutrophilic infiltration and gastric metaplasia was found in 20 patients (27%) taking NSAIDs, compared with 56 patients (57%) not taking NSAIDs (chi 2 = 16.24, p < 0.001). This degree of duodenitis was also found in 20 of 25 patients (80%) with duodenal ulcers, regardless of NSAID intake (chi 2 = 15.38, p < 0.001). Gastric metaplasia was identified in 20 patients (27%) receiving NSAIDs and 38 (39%) not receiving NSAIDs. Duodenal H pylori was only seen in patients with gastric metaplasia 10 (50%) receiving NSAIDs, and 34 (89%) not receiving NSAIDs. H pylori positive gastritis, and the combination of active duodenitis and gastric metaplasia were independent predictors of duodenal ulceration. It is concluded that active duodenitis is less common in patients taking NSAIDs but is strongly associated with gastric metaplasia, H pylori positive gastritis, and duodenal ulceration. These findings are relevant to the pathogenesis and treatment of duodenal ulcers in patients taking NSAIDs.
中文摘要： 十二指肠炎和胃化生通常被幽门螺杆菌 (H pylori) 定植，它们在十二指肠溃疡发病机理中的重要性日益得到认可。在接受非甾体抗炎药 (NSAIDs) 的患者中，这种情况尚不清楚，这些患者具有较高的消化性溃疡风险。这项研究的目的是确定是否存在NSAIDs，幽门螺杆菌和十二指肠溃疡的十二指肠组织学异常。内镜十二指肠活检标本取自172例患者的健康黏膜 (74例服用NSAIDs，98例未服用)。根据嗜中性粒细胞和浆细胞浸润，绒毛高度，布鲁纳氏腺脱垂和胃化生的程度对十二指肠炎进行分级。十二指肠炎的活动取决于嗜中性粒细胞浸润。构建了涵盖所有上述因素的全局评分，并且还评估了胃和十二指肠中的幽门螺杆菌。结果表明，服用NSAIDs的20例 (27% 例) 中发现十二指肠炎有不同程度的中性粒细胞浸润和胃上皮化生，而未服用NSAIDs的56例 (57% 例) 中发现十二指肠炎 (chi 2 = 16.24，p <0.001)。无论NSAID摄入量如何，25例十二指肠溃疡患者中有20例 (80% 例) 也发现了这种程度的十二指肠炎 (chi 2 = 15.38，p <0.001)。在接受NSAIDs的20例患者 (27% 例) 和未接受NSAIDs的38例患者 (39% 例) 中确定了胃上皮化生。十二指肠幽门螺杆菌仅见于接受NSAIDs的胃化生10 (50%) 和未接受NSAIDs的34 (89%) 患者。幽门螺杆菌阳性胃炎，活动性十二指肠炎和胃上皮化生的组合是十二指肠溃疡的独立预测因子。结论是，活动性十二指肠炎在服用NSAIDs的患者中较少见，但与胃上皮化生，幽门螺杆菌阳性胃炎和十二指肠溃疡密切相关。这些发现与服用NSAIDs的患者十二指肠溃疡的发病机理和治疗有关。
发表日期： 1993 Sep
中文标题： 阿莫西林联合奥美拉唑与三联疗法根除十二指肠溃疡幽门螺杆菌的比较: 一项前瞻性、随机、对照研究.
论文作者： Labenz, J; Gyenes, E; Rühl, G H; Börsch, G
英文摘要： Treatment with amoxicillin and omeprazole resulted in encouraging Helicobacter pylori eradication rates in pilot studies that included medium term follow up. These results were evaluated in a prospective, randomised and controlled study. Forty patients with active duodenal ulcer disease and H pylori colonisation of the gastric mucosa were randomly assigned to receive either omeprazole (20 mg twice daily) and amoxicillin suspension (500 mg four times daily) for two weeks (group I) or bismuth subsalicylate (600 mg three times daily), metronidazole (400 mg three times daily), tetracycline (500 mg three times daily), and ranitidine (300 mg in the evening) for two weeks (group II). Study medication was followed in both groups by a four week treatment course with 300 mg ranitidine up to the final examination. One patient from each group was lost to follow up. H pylori was eradicated in 78.9% of group I and 84.2% of group II (p = 1.00). All ulcers in patients on omeprazole plus amoxicillin healed but in the triple treatment group four patients had residual peptic lesions after six weeks (ulcer healing rate: 78.9%, p = 0.11). Complete pain relief occurred after a median duration of 1 day in group I and of 6 days in group II (p = 0.03). There were no major complications in either group but minor side effects were more frequently recorded in patients on triple therapy (63.2% v 15.8%, p < 0.01). In conclusion, two weeks of treatment with omeprazole plus amoxicillin is as good as triple therapy plus ranitidine in eradicating H pylori but seems better with regard to safety, pain relief, and ulcer healing. Thus, amoxicillin plus omeprazole should be recommended as the treatment of choice in eradicating H pylori in patients with duodenal ulcer disease.
中文摘要： 在包括中期随访在内的试点研究中，阿莫西林和奥美拉唑治疗可提高幽门螺杆菌的根除率。这些结果在前瞻性，随机和对照研究中进行了评估。40名患有活动性十二指肠溃疡疾病和胃粘膜幽门螺杆菌定植的患者被随机分配接受奥美拉唑 (20 mg，每日两次) 和阿莫西林混悬液 (500 mg，每日四次) 治疗两周 (I组) 或水杨酸铋 (600 mg，每日三次)，甲硝唑(每天三次400毫克)，四环素 (每天三次500毫克) 和雷尼替丁 (晚上300毫克) 持续两周 (II组)。在两组中，研究药物均以300毫克雷尼替丁进行为期四周的治疗，直至最终检查。每组有一名患者失访。I组78.9% 根除幽门螺杆菌，II组84.2% 根除幽门螺杆菌 (p = 1.00)。接受奥美拉唑加阿莫西林治疗的患者的所有溃疡均愈合，但在三联治疗组中，有4例患者在六周后有残余的消化性溃疡 (溃疡治愈率: 78.9%，p = 0.11)。I组的中位持续时间为1天后，II组的中位持续时间为6天后，疼痛完全缓解 (p = 0.03)。两组均未出现严重并发症，但在三联疗法的患者中更常见地记录到轻微的副作用 (63.2% v 15.8%，p <0.01)。总之，在根除幽门螺杆菌方面，奥美拉唑加阿莫西林治疗两周与三联疗法加雷尼替丁治疗一样好，但在安全性，疼痛缓解和溃疡愈合方面似乎更好。因此，建议将阿莫西林加奥美拉唑作为根除十二指肠溃疡患者幽门螺杆菌的首选治疗方法。
发表日期： 1993 Sep
中文标题： 第十三届Gaddum纪念讲座。调节胃微循环的神经元和内皮衍生介质: 平衡的完整性。
论文作者： Whittle, B. J.
英文摘要： Images: Figure 2Figure 3Figure 4
中文摘要： 图像: 图2图3图4
发表日期： 1993 Sep
英文标题： In Vitro Susceptibility of Helicobacter pylori to Several Antimicrobial Combinations.[全文]
中文标题： 幽门螺杆菌对几种抗菌药物组合的体外敏感性。
论文作者： Pavičić, M. J. A. M. P.; Namavar, F.; Verboom, T.; van Winkelhoff, A. J.; de Graaff, J.
英文摘要：
中文摘要：
发表日期： 1993 Oct 30
中文标题： 新药在英国一个地区的市场渗透: 对全科医生和管理人员的影响。
论文作者： McGavock, H; Webb, C H; Johnston, G D; Milligan, E
英文摘要： OBJECTIVE--To determine the use of new drugs in one United Kingdom region. DESIGN--Examination of data on prescribing of angiotensin converting enzyme inhibitors, new broad spectrum antibiotics, and H2 receptor antagonists. Calculation of number of defined daily doses prescribed each month. SETTING--All general practices in Northern Ireland. MAIN OUTCOME MEASURES--Drug use index and market share of each drug. RESULTS--During 1988-91 prescribing of angiotensin converting enzyme inhibitors increased by 126%, of H2 receptor antagonists by 46%, and of new antibiotics by 207%. The first drug on the market usually retained the largest market share. Use of oral antibiotics increased threefold irrespective of the reporting policy of the general practitioners' local laboratory. CONCLUSIONS--The increase in prescribing of these drugs seems to be greater than can be accounted for by an increase in patients with specific indications for these drugs. This suggests that the profession has not instituted effective checks to ensure that the legitimate promotion of new products does not lead to inappropriate and wasteful use.
中文摘要： 目的-确定一个英国地区的新药使用情况。设计-检查血管紧张素转换酶抑制剂，新型广谱抗生素和H2受体拮抗剂的处方数据。计算每月规定的每日剂量的数量。设置-北爱尔兰的所有一般做法。主要结果指标-药物使用指数和每种药物的市场份额。结果-在1988-91年间，血管紧张素转化酶抑制剂的处方增加了126% %，H2受体拮抗剂的处方增加了46% %，新抗生素的处方增加了207% %。市场上的第一种药物通常保留最大的市场份额。无论全科医生当地实验室的报告政策如何，口服抗生素的使用增加了三倍。结论-这些药物处方的增加似乎大于具有这些药物特定适应症的患者的增加。这表明该行业尚未进行有效的检查，以确保合法推广新产品不会导致不适当和浪费的使用。
发表日期： 1993 Oct
英文标题： Housing in early life and later mortality.[全文]
中文标题： 早年的住房和后来的死亡。
论文作者： Coggon, D; Barker, D J; Inskip, H; Wield, G
英文摘要： STUDY OBJECTIVES--The aim was to examine the influence of domestic crowding and household amenities in early life on later mortality from all causes and specifically from stomach cancer, chronic obstructive pulmonary disease, and rheumatic heart disease. DESIGN--This was a retrospective cohort study of people whose houses had been surveyed in 1936 and whose household size was known from the 1939 census. Subjects were followed through the National Health Service Central Register from 1951 to 1989. SETTING--The housing survey had been carried out in the midland town of Chesterfield. SUBJECTS--Subjects comprised 8138 men and women born after 1900. RESULTS--A total of 2929 deaths were observed during the follow up period. All causes mortality in the full cohort was not consistently related to any of the housing variables examined, but among subjects who were still children at the time of the housing survey, death rates were higher in those whose houses were crowded or lacked a hot water tap. No associations could be shown between stomach cancer and domestic crowding or food storage facilities; chronic obstructive pulmonary disease and domestic crowding or use of gas for cooking; or rheumatic heart disease and domestic crowding. There were few deaths from these causes, however, in subjects who were children at the time of housing survey. CONCLUSIONS--The findings suggest that the housing of young adults in Chesterfield during the 1930s had little effect on their later mortality. Further follow up of the cohort is needed before firm conclusions can be drawn about the influence of housing at younger ages.
中文摘要： 研究目标-目的是研究早期生活中家庭拥挤和家庭便利设施对各种原因，特别是胃癌，慢性阻塞性肺疾病和风湿性心脏病的后期死亡率的影响。设计-这是一项回顾性队列研究，研究对象是1936年对房屋进行过调查的人，其家庭规模可从1939人口普查中得知。从1951年到1989年，通过国家卫生服务中心登记册对受试者进行了随访。背景 -- 住房调查是在中部城镇切斯特菲尔德进行的。受试者-受试者包括1900年后出生的8138名男性和女性。RESULTS--A随访期间共观察到2929例死亡。在整个队列中，所有原因的死亡率与所检查的任何住房变量都不一致相关，但是在住房调查时仍然是孩子的受试者中，房屋拥挤或缺乏热的死亡率更高水龙头。胃癌与家庭拥挤或食物储存设施之间没有关联; 慢性阻塞性肺疾病和家庭拥挤或使用天然气做饭; 或风湿性心脏病和家庭拥挤。然而，在住房调查时，这些原因导致的死亡人数很少。结论-研究结果表明，19 30年代切斯特菲尔德的年轻人的住房对他们后来的死亡率影响不大。在得出关于年轻时住房影响的确切结论之前，需要对该队列进行进一步的跟进。
发表日期： 1993 Oct
英文标题： Comparison of commercial diagnostic tests for Helicobacter pylori antibodies.[全文]
中文标题： 幽门螺杆菌抗体商业诊断试验的比较。
论文作者： Schembri, M A; Lin, S K; Lambert, J R
英文摘要： A number of serological tests measuring the presence of Helicobacter pylori-specific serum immunoglobulin G (IgG) are now commercially available. The aim of this study was to evaluate the clinical accuracy of five commercial H. pylori antibody tests: GAP-IgG (Biomerica), HELpTEST (AMRAD, Kew, Victoria, Australia), HELICO-G (Porton Cambridge), Pyloriset (Orion Diagnostica), and ROCHE (Roche Diagnostics). A total of 162 subjects presenting for routine upper endoscopy were studied. H. pylori was diagnosed if culture, histology, or both were positive. Ten milliliters of venous blood was collected at the time of endoscopy for serological assessment. The sensitivity and specificity of each test (GAP-IgG, HELpTEST, HELICO-G, Pyloriset, and ROCHE) were as follows: 83 and 79%, 92 and 77%, 86 and 65%, 89 and 56%, and 98 and 69%, respectively. Positive and negative predictive values were 97 and 83%, 90 and 91%, 76 and 83%, 68 and 84%, and 86 and 97%, respectively. The specificity of most tests increased by approximately 10% when sera from subjects less than 45 years old were examined. The number of sera falling into the grey zone for each test (an indeterminate result with respect to H. pylori status) varied between 2.5 and 19%. This study highlights the need for all serological kits to be independently evaluated on the population to be studied by testing against a microbiologically defined panel of H. pylori-positive and -negative sera.
中文摘要： 现在可以商购获得许多用于测量幽门螺杆菌特异性血清免疫球蛋白G (IgG) 存在的血清学测试。这项研究的目的是评估五种商业幽门螺杆菌抗体测试的临床准确性: gap-igg (Biomerica)，HELpTEST (AMRAD，Kew，Victoria，澳大利亚)，helico-g (Porton Cambridge)，pylorisa (Orion Diagnostica) 和ROCHE (Roche Diagnostics)。总共研究了162例常规上内镜检查的受试者。如果培养、组织学或两者均为阳性，则诊断为幽门螺杆菌。在内窥镜检查时收集10毫升静脉血用于血清学评估。每个测试 (gap-igg，HELpTEST，helic0-g，pylorset和ROCHE) 的敏感性和特异性如下: 分别为83和79%，92和77%，86和65%，89和56% 以及98和69%。阳性和阴性预测值分别为97和83%，90和91%，76和83%，68和84% 以及86和97%。当检查来自小于45岁的受试者的血清时，大多数测试的特异性增加了约10%。每次测试落入灰色区域的血清数量 (关于幽门螺杆菌状态的不确定结果) 在2.5和19% 之间变化。这项研究强调了需要通过针对微生物学定义的幽门螺杆菌阳性和阴性血清组进行测试，对所有血清学试剂盒进行独立评估。
发表日期： 1993 Oct
英文标题： Isolation of Helicobacter pylori from saliva.[全文]
中文标题： 从唾液中隔离幽门螺杆菌。
论文作者： Ferguson, D A; Li, C; Patel, N R; Mayberry, W R; Chi, D S; Thomas, E
英文摘要： Helicobacter pylori was grown in low numbers from the saliva of one of nine patients who were positive for gastric H. pylori. The saliva-derived isolate from this patient was identical to the antral biopsy-derived isolate from the same patient and differed from isolates cultured from the antral biopsies of all other patients by soluble-protein electrophoresis, restriction endonuclease DNA analysis, and Southern blot hybridization. This is the first observation, to our knowledge, of the recovery of viable H. pylori from saliva.Images:
中文摘要： 幽门螺杆菌从9名胃幽门螺杆菌阳性患者之一的唾液中少量生长。来自该患者的唾液来源的分离物与来自同一患者的肛门活检来源的分离物相同，并且与通过可溶性蛋白电泳，限制性核酸内切酶DNA分析和Southern印迹杂交从所有其他患者的肛门活检培养的分离物不同。据我们所知，这是从唾液中恢复活幽门螺杆菌的第一个观察结果。图片:
发表日期： 1993 Oct
英文标题： Adherence of Helicobacter pylori to primary human gastrointestinal cells.[全文]
中文标题： 幽门螺杆菌对原代人胃肠道细胞的粘附。
论文作者： Clyne, M; Drumm, B
英文摘要： Helicobacter pylori adheres only to gastric cells in vivo. However, the organism adheres to a wide variety of nongastric cells in vitro. In this study, we have used flow cytometry to assess the adherence of H. pylori to primary epithelial cells isolated from gastric, duodenal, and colonic biopsy specimens by collagenase digestion. After incubation of bacteria and cells together and subsequent staining with a two-stage fluorescein isothiocyanate-labelled H. pylori antibody method, cells with adherent bacteria could be easily distinguished from cells without bacteria. Binding to Kato III cells (a gastric adenocarcinoma cell line) was saturable when bacteria and cells were mixed at a ratio of 250:1. Adherence to cells isolated from gastric biopsy specimens was significantly better than adherence to cells isolated from duodenal or colonic biopsy specimens. Almost 70% of gastric cells had bacteria bound, in contrast to 30% of duodenal cells and 32% of colonic cells (P < 0.0001). There was no correlation between expression of hemagglutinins by the bacteria and ability to bind to either Kato III cells or primary epithelial cells isolated from gastric biopsy specimens. In view of the strict tropism that the organism exhibits in vivo for gastric cells, the results of this study indicate that primary cells are ideal for assessing the factors that might play a role in the pathogenesis of disease caused by the organism.Images:
中文摘要： 幽门螺杆菌在体内仅粘附于胃细胞。然而，该生物体在体外粘附于多种非胃细胞。在这项研究中，我们使用流式细胞术评估了幽门螺杆菌对通过胶原酶消化从胃，十二指肠和结肠活检标本中分离的原代上皮细胞的粘附。在将细菌和细胞一起孵育并随后用两阶段异硫氰酸荧光素标记的幽门螺杆菌抗体方法染色后，可以容易地将具有粘附细菌的细胞与没有细菌的细胞区分开。当细菌和细胞以250:1的比例混合时，与Kato III细胞 (胃腺癌细胞系) 的结合是饱和的。对从胃活检标本中分离的细胞的粘附性明显优于对从十二指肠或结肠活检标本中分离的细胞的粘附性。与十二指肠细胞的30% 和结肠细胞的32% 相比，几乎70% 的胃细胞有细菌结合 (P <0.0001)。细菌对血凝素的表达与与从胃活检标本中分离出的Kato III细胞或原代上皮细胞结合的能力之间没有相关性。鉴于生物体在体内对胃细胞表现出严格的向性，这项研究的结果表明，原代细胞对于评估可能在由生物体引起的疾病的发病机理中起作用的因素是理想的。
发表日期： 1993 Oct
中文标题： 从各种动物的胃获得的原代培养物中幽门螺杆菌对胃上皮细胞的粘附。
论文作者： Kobayashi, Y; Okazaki, K; Murakami, K
英文摘要： Of 35 strains of Helicobacter pylori tested, 5 were found to adhere well to HEp-2 cells. We selected three of these adhesive strains and four from the remaining strains to examine their ability to adhere to gastric epithelial cells in primary cultures obtained by collagenase digestion of stomachs from mice, rats, Mongolian gerbils, guinea pigs, pigs, and cynomolgus monkeys. The three adhesive strains adhered well to epithelial cells from monkey and pig gastric antra. The adhesion was inhibited by incubating the bacterial cells with fetuin, and this inhibition was further confirmed by the binding of gold-labeled fetuin to the surface of the adhesive strains. However, these adhesive strains only weakly adhered to fundic epithelial cells from monkeys and pigs and to gastric epithelial cells from the other animals. As for the four strains poorly adhesive to HEp-2 cells, they adhered weakly to gastric epithelial cells from all of the animals tested. They had higher hemagglutination titers than the adhesive strains, showing that there was no correlation between hemagglutination titers and the ability to adhere to gastric cells. Taking the similarities of human and monkey or pig stomachs into consideration, these results suggest that the primary target cell of H. pylori in colonization in human stomachs is the antral epithelial cell and that the putative adhesin involved in adhesion has affinity for fetuin.Images:
中文摘要： 在测试的35种幽门螺杆菌菌株中，发现5种与HEp-2细胞粘附良好。我们选择了这些粘附菌株中的三个，并从其余菌株中选择了四个，以检查它们在通过胶原酶消化小鼠，大鼠，蒙古沙鼠，豚鼠，猪和食蟹猴的胃而获得的原代培养物中粘附胃上皮细胞的能力。这三种粘合剂菌株很好地粘附到来自猴和猪胃窦的上皮细胞上。通过将细菌细胞与胎蛋白一起孵育来抑制粘附，并且通过金标记的胎蛋白与粘附菌株表面的结合进一步证实了这种抑制。但是，这些粘附菌株仅微弱地粘附于猴子和猪的胃底上皮细胞以及其他动物的胃上皮细胞。至于四种菌株对HEp-2细胞的粘附性较差，它们对所有测试动物的胃上皮细胞的粘附性较弱。它们的血凝滴度高于粘附菌株，表明血凝滴度与粘附胃细胞的能力之间没有相关性。考虑到人和猴或猪胃的相似性，这些结果表明，幽门螺杆菌在人胃定植中的主要靶细胞是胃窦上皮细胞，并且与粘附有关的假定粘附素对胎球蛋白具有亲和力。
发表日期： 1993 Oct
英文标题： Duodenal ulcer pain--the role of acid and inflammation.[全文]
中文标题： 十二指肠溃疡疼痛-酸和炎症的作用。
论文作者： McColl, K E; Fullarton, G M
英文摘要：
中文摘要：
发表日期： 1993 Oct
中文标题： 消化性溃疡病胃十二指肠粘膜的组织型纤溶酶原激活物活性降低。
论文作者： Wodziński, M A; Bardhan, K D; Reilly, J T; Cooper, P; Preston, F E
英文摘要： The gastroduodenal mucosa has a rich blood supply. An active fibrinolytic system is presumably required to maintain vascular patency, and impairment may result in reduced blood flow, focal tissue necrosis, and peptic ulcerogenesis. Tissue type and urokinase type plasminogen activator activity (expressed as mIU/mg protein) and plasminogen activator inhibitor type-1 antigen were assayed in homogenates of gastric and duodenal biopsy specimens taken from patients with: normal endoscopy (controls) (n = 14); active duodenal ulcer (n = 21); healed duodenal ulcer (n = 12); and active benign gastric ulcer (n = 15). In controls mean duodenal tissue type plasminogen activator activity was 4110 and urokinase type plasminogen activator activity 150; gastric tissue type plasminogen activator was 2760 and urokinase type plasminogen activator 170; plasminogen activator inhibitor type-1 was generally undetectable. At the edge of active duodenal ulcers tissue type plasminogen activator was considerably reduced, 2220 (p < 0.001) whereas urokinase type plasminogen activator was raised, 290 (p < 0.01). At the edge of active benign gastric ulcers tissue type plasminogen activator was substantially reduced, 1160 (p < 0.001) but urokinase type plasminogen activator was unchanged. At the scar of healed duodenal ulcers tissue type plasminogen activator was slightly reduced, 3290, but urokinase type plasminogen activator was increased, 308 (p < 0.05). H2 receptor antagonist treatment had little effect on tissue type or urokinase type plasminogen activator activity. Plasminogen activator inhibitor type-1 was increased at the edge of active ulcers (p < 0.05) especially when tissue type plasminogen activity was low (r = -0.61, p < 0.05). These findings are consistent with the hypothesis that impaired fibrinolytic activity may be implicated in peptic ulcerogenesis.
中文摘要： 胃十二指肠粘膜有丰富的血液供应。可能需要活跃的纤维蛋白溶解系统来维持血管通畅，并且受损可能导致血流量减少，局灶性组织坏死和消化性溃疡发生。在以下患者的胃和十二指肠活检标本匀浆中测定了组织类型和尿激酶型纤溶酶原激活物活性 (以mIU/mg蛋白表示) 和纤溶酶原激活物抑制剂1型抗原: 正常内窥镜检查 (对照) (n = 14); 活动性十二指肠溃疡 (n = 21)；十二指肠溃疡愈合(n = 12); 和活动性良性胃溃疡 (n = 15)。在对照组中，平均十二指肠组织型纤溶酶原激活物活性4110，尿激酶型纤溶酶原激活物活性150; 胃组织型纤溶酶原激活物2760，尿激酶型纤溶酶原激活物170; 纤溶酶原激活物抑制剂1型通常检测不到。在活动性十二指肠溃疡的边缘，组织型纤溶酶原激活物显着降低，2220 (p <0.001)，而尿激酶型纤溶酶原激活物升高，290 (p <0.01)。在活动性良性胃溃疡的边缘，组织型纤溶酶原激活剂显着减少，1160 (p <0.001)，但尿激酶型纤溶酶原激活剂没有变化。十二指肠溃疡愈合后的瘢痕处组织型纤溶酶原激活物略有减少，3290，但尿激酶型纤溶酶原激活物增加，308 (p <0.05)。H2受体拮抗剂治疗对组织型或尿激酶型纤溶酶原激活物活性几乎没有影响。1型纤溶酶原激活物抑制剂在活动性溃疡边缘增加 (p <0.05)，尤其是当组织型纤溶酶原活性低时 (r = -0.61，p <0.05)。这些发现与纤维蛋白溶解活性受损可能与消化性溃疡发生有关的假设一致。
发表日期： 1993 Oct
中文标题： 血清胃蛋白酶原C在血清胃蛋白酶原A浓度升高患者中的诊断价值。
论文作者： Biemond, I; Kreuning, J; Jansen, J B; Lamers, C B
英文摘要： Hypopepsinogenaemia A is often found in patients with gastric atrophy and gastric surgery. In these conditions serum pepsinogen C provides additional diagnostic information, especially when expressed as pepsinogen A:C ratio. Hyperpepsinogenaemia A has been shown in patients with duodenal ulcer disease, Zollinger-Ellison syndrome, hypertrophic gastropathy, chronic renal failure, and during omeprazole treatment. As patients with hyperpepsinogenaemia A often overlap in symptoms, endoscopical findings, and serum gastrin values, this study has evaluated whether measurement of serum pepsinogen C in subjects with hyperpepsinogenaemia A can help in differentiating clinical conditions. Serum concentrations of pepsinogen A and C were measured in serologically Helicobacter pylori negative blood transfusion donors (127) as reference population, and in patients with Zollinger-Ellison syndrome (24), duodenal ulcer (50), hypertrophic gastropathy (5), and chronic renal failure (50), and also in reflux oesophagitis patients on longterm omeprazole treatment (28). A low pepsinogen A:C ratio was found in all patients with hypertrophic gastropathy. A pepsinogen A:C ratio above the critical value of 4.7 was found in 14 (70.0%) of the Zollinger-Ellison patients, two (9.5%) of the duodenal ulcer patients, 11 (25.6%) of the patients with chronic renal failure, and in one (7.1%) of the patients receiving longterm omeprazole treatment. In fact, all but three hyperpepsinogenaemia A patients with a pepsinogen A:C ratio greater than 4.7 and normal renal function had the Zollinger-Ellison syndrome. In patients with hyperpepsinogenaemia A, a low pepsinogen A:C ratio may point to hypertrophic gastropathy, while a pepsinogen A:C ratio greater than 4.7 is suggestive for the Zollinger-Ellison syndrome.
中文摘要： 在胃萎缩和胃手术的患者中经常发现低胃泌素血症A。在这些情况下，血清胃蛋白酶原C提供了额外的诊断信息，特别是当以胃蛋白酶原A:C比例表示时。在十二指肠溃疡疾病，zollinger-ellison综合征，肥大性胃病，慢性肾功能衰竭以及奥美拉唑治疗期间，已显示出高胃蛋白酶原血症A。由于高胃蛋白酶原A患者的症状，内镜检查结果和血清胃泌素值经常重叠，因此本研究评估了高胃蛋白酶原A患者血清胃蛋白酶原C的测量是否有助于区分临床状况。血清胃蛋白酶原A和C的浓度在血清学幽门螺杆菌阴性的输血献血者 (127) 作为参考人群，并在zollinger-ellison综合征 (24)，十二指肠溃疡 (50)，肥大性胃病 (5) 和慢性肾功能衰竭 (50)，并且还在长期奥美拉唑治疗的反流性食管炎患者中 (28)。在所有肥厚性胃病患者中发现低的胃蛋白酶原A:C比。在14名 (70.0%) 的zollinger-ellison患者，2名 (9.5%) 的十二指肠溃疡患者，11名 (25.6%) 的慢性肾功能衰竭患者中发现胃蛋白酶原A:C比值高于4.7的临界值，其中一个 (7.1%) 接受长期奥美拉唑治疗的患者。实际上，除3例胃蛋白酶原A:C比值大于4.7且肾功能正常的高胃蛋白酶原a患者外，所有患者均患有zollinger-ellison综合征。在患有高胃蛋白酶原血症A的患者中，低的胃蛋白酶原a: C比率可能表明肥大性胃病，而胃蛋白酶原A:C比率大于4.7则提示zollinger-ellison综合征。
发表日期： 1993 Oct
中文标题： 与复发性十二指肠溃疡 (RUDER) 延迟愈合相关的危险因素的前瞻性多中心研究。鲁德研究小组。
论文作者： Armstrong, D; Arnold, R; Classen, M; Fischer, M; Goebell, H; Blum, A L
英文摘要： Risk factors for delayed duodenal ulcer healing during treatment with ranitidine (300 mg daily) were examined in a multicentre German study of 1923 patients with endoscopically proved, recurrent duodenal ulceration. Healing rates, per protocol, were 39.5% at two weeks, 70.9% at four weeks, and 93.2% at eight weeks. Prospective testing of five, predefined risk factors indicated that smoking (p = 0.0039) was associated with a decreased healing rate at two weeks. Frequent prior recurrence (p = 0.464), a heavy physical workload (p = 0.145), and psychological stress (p = 0.062) were not associated with a decreased healing rate and there were too few patients at risk to allow assessment of the effect of regular NSAID intake. Exploratory analysis identified prior slow healing, a large ulcer, multiple ulcers, and prior ulcer complications, in addition to smoking, as markers of slow healing. In the absence of these risk factors, the mean healing time was 3.3 weeks (95% confidence interval 3.0, 3.5), rising to 3.7 weeks (3.5, 3.9) for one, 4.4 weeks (4.1, 4.7) for two, and 5.1 weeks (4.5, 5.6) for three to five risk factors. Delayed duodenal ulcer healing is associated with multiple factors whose effect is cumulative; for patients with two or more of five easily identified risk factors, more than four weeks' treatment with a histamine H2 receptor antagonist is required to achieve ulcer healing.
中文摘要： 在一项多中心的德国研究中，研究了雷尼替丁 (每天300 mg) 治疗期间十二指肠溃疡愈合延迟的危险因素，该研究对1923例经内镜证实为复发性十二指肠溃疡的患者进行了研究。根据方案，愈合率在两周时39.5%，在四周时70.9%，在八周时93.2%。对五个预先确定的危险因素进行的前瞻性测试表明，吸烟 (p = 0.0039) 与两周时的治愈率降低有关。频繁的先前复发 (p = 0.464)，沉重的体力劳动 (p = 0.145) 和心理压力 (p = 0.062) 与治愈率降低无关，并且处于风险中的患者太少，无法评估影响定期摄入NSAID。探索性分析确定了除吸烟外，先前的缓慢愈合，大溃疡，多发性溃疡和先前的溃疡并发症是缓慢愈合的标志。在没有这些危险因素的情况下，平均愈合时间为3.3周 (95% 置信区间3.0，3.5)，1周上升至3.7周 (3.5，3.9)，4.4周 (4.1，4.7)，5.1周 (4.5，5.6) 三到五个风险因素。十二指肠溃疡愈合延迟与多种因素有关，这些因素的影响是累积的; 对于具有五个容易识别的危险因素中的两个或更多个的患者，需要使用组胺H2受体拮抗剂治疗超过四周才能实现溃疡愈合。
发表日期： 1993 Oct
英文标题： Post-bulbar and coexisting ulceration: unique features of peptic ulcer in Hyderabad.[全文]
中文标题： 球后溃疡和并存溃疡: 海得拉巴消化性溃疡的独特特征。
论文作者： Rao, S S; Murthy, K V
英文摘要： Post-bulbar ulceration is uncommon, but a pilot study in Hyderabad showed a high incidence. We therefore carried out a prospective endoscopic study of the distribution of peptic ulceration and its relation to symptoms and demography. Of the 360 consecutive patients referred for endoscopy, 113 (92 men, 21 women) had peptic ulceration. Median age 35 years, median duration one year. Five patients (4%) had gastric ulcer, 77 (68%) had duodenal ulcer, and 31 (28%) had coexisting gastric and duodenal ulcer. The duodenal ulcer was found in the pyloric canal in 14% of patients, in the bulb in 80%, and the post-bulbar region in 56% of patients. Sixty seven per cent of duodenal ulcers were located at more than one site. The incidence of post-bulbar v bulbar ulcer was 1:1.5. Deformed bulb was seen in 50% of duodenal ulcer patients, but haemorrhage and stenosis were uncommon. Except for nocturnal pain, there were no differences in symptoms between the groups. Forty two per cent of patients smoked, 15% chewed tobacco, and 18% drank alcohol; almost all were men. Sixty four per cent drank tea. The staple diet (85%) was rice, and 70% used tamarind and spices daily. Duodenal ulcer was three times more common than gastric ulcer with a high incidence of post-bulbar and coexisting ulcer. It affected a younger and predominantly male population, and was not associated with a higher rate of complication.
中文摘要： 球部后溃疡并不常见，但在海得拉巴的一项初步研究显示发病率很高。因此，我们对消化性溃疡的分布及其与症状和人口统计学的关系进行了前瞻性内窥镜研究。在360例接受内窥镜检查的连续患者中，113例 (92例男性，21例女性) 患有消化性溃疡。中位年龄35岁，中位病程1年。5例 (4%) 患有胃溃疡，77例 (68%) 患有十二指肠溃疡，31例 (28%) 患有胃和十二指肠溃疡。在14% 例患者的幽门管，80% 例患者的球部和56% 例患者的球部后区域发现了十二指肠溃疡。67% 的十二指肠溃疡位于一个以上的部位。球后v球部溃疡的发生率为1:1.5。50% 十二指肠溃疡患者可见球茎变形，但出血和狭窄并不常见。除夜间疼痛外，两组之间的症状没有差异。42% 的患者吸烟，15% 咀嚼烟草，18% 饮酒; 几乎所有人都是男性。64% 的人喝茶。主食 (85%) 是大米，70% 每天使用罗望子和香料。十二指肠溃疡的发生率是胃溃疡的三倍，球部后溃疡和并存溃疡的发生率很高。它影响了年轻且主要是男性的人群，并且与较高的并发症发生率无关。
发表日期： 1993 Oct
英文标题： Systemic and mucosal humoral responses to Helicobacter pylori in gastric cancer.[全文]
中文标题： 胃癌对幽门螺杆菌的全身和粘膜体液反应。
论文作者： Crabtree, J E; Wyatt, J I; Sobala, G M; Miller, G; Tompkins, D S; Primrose, J N; Morgan, A G
英文摘要： The systemic IgG response to Helicobacter pylori was examined in 70 patients with gastric cancer. H pylori IgG antibodies were assayed by enzyme linked immunosorbent assay (ELISA), and serological recognition of H pylori antigens was characterised by western blotting. A percentage of 78.5 were seropositive by ELISA. Two of five patients under age 50 were seronegative. Positivity was unrelated to age, sex, tumour type, or site. Ninety one per cent of ELISA positive cancer patients recognised the H pylori cytotoxin associated 120 kilodalton (kD) protein, significantly more than a control group of 47 ELISA positive patients with non-ulcer dyspepsia (72%). Four of 15 ELISA negative cancer patients also showed recognition of this protein in western blots. Mucosal IgA responses to H pylori were examined by immunoblotting supernatants of in vitro cultured resected antral mucosa in an overlapping group of 19 gastric cancer patients. Eighteen had a positive response, including 10 of 11 negative for H pylori by biopsy urease testing. The systemic and local immunoblotting results show that the high seroprevalence of H pylori antibodies detected by ELISA is nevertheless an underestimate of past infection. Dyspepsia screening policies based solely on H pylori ELISA would miss some young patients with gastric cancer. Further study of the relation of the H pylori cytotoxin to gastric precancerous lesions is warranted.Images: Figure 1Figure 2Figure 3Figure 4
中文摘要： 在70例胃癌患者中检查了对幽门螺杆菌的全身性IgG反应。通过酶联免疫吸附测定 (ELISA) 测定幽门螺杆菌IgG抗体，并通过蛋白质印迹法表征幽门螺杆菌抗原的血清学识别。通过ELISA，一定百分比的78.5是血清阳性的。50岁以下的五名患者中有两名血清阴性。阳性与年龄、性别、肿瘤类型或部位无关。91% 的ELISA阳性癌症患者识别出幽门螺杆菌细胞毒素相关的120千道尔顿 (kD) 蛋白，明显高于47例非溃疡性消化不良的ELISA阳性患者的对照组 (72%)。15名ELISA阴性癌症患者中有4名在蛋白质印迹中也显示出该蛋白质的识别。通过对19例胃癌患者的重叠组中体外培养的切除的胃窦粘膜的上清液进行免疫印迹，检查了粘膜IgA对幽门螺杆菌的反应。18例有阳性反应，包括活检尿素酶检测为幽门螺杆菌阴性的11例中的10例。全身和局部免疫印迹结果表明，通过ELISA检测到的幽门螺杆菌抗体的高血清阳性率仍然低估了过去的感染。仅基于幽门螺杆菌ELISA的消化不良筛查政策会遗漏一些年轻的胃癌患者。需要进一步研究幽门螺杆菌细胞毒素与胃癌前病变的关系。图像: 图1图2图3图4
发表日期： 1993 Oct
英文标题： Acute perforated duodenal ulcer is not associated with Helicobacter pylori infection.[全文]
中文标题： 急性十二指肠溃疡穿孔与幽门螺杆菌感染无关。
论文作者： Reinbach, D H; Cruickshank, G; McColl, K E
英文摘要： Most patients with chronic duodenal ulcer disease have Helicobacter pylori infection and eradicating it considerably reduces the relapse rate. The prevalence of H pylori in 80 patients (mean age = 52 years, range 17-85) presenting with acute perforated duodenal ulcer was examined and compared with age and sex matched hospital control patients. H pylori state was assessed by serum anti-H pylori IgG (Helico-G kit, Porton) using a titre of 18 or less as negative with a specificity of 89% and sensitivity of 88%. Only 47% of the perforated duodenal ulcer patients were positive for H pylori and this was similar to the value of 50% in the controls. In 51 of the perforated duodenal ulcer patients 14C-urea breath tests were also performed 4-10 weeks after surgery and this confirmed that only 49% were positive for H pylori. None of these patients had received perioperative drugs that might have eradicated the infection. The H pylori positive and H pylori negative perforated duodenal ulcer patients were similar with respect to age (53, 51), smoking (84%, 83%), and consumption of more than 15 units of alcohol per week (42%, 38%). Duodenal ulcer disease had been diagnosed before acute perforation in only 24% of those with H pylori and also 24% of those without the infection. Regular non-steroidal anti-inflammatory drug (NSAID) use was common in both those with (44%) and without (45%) H pylori. In conclusion, the lack of association of acute perforated duodenal ulcer and H pylori infection suggests that perforated duodenal ulcer has a different pathogenesis from chronic duodenal ulcer disease, and that the first should not be regarded simply as a complication of the second.
中文摘要： 大多数患有慢性十二指肠溃疡疾病的患者都有幽门螺杆菌感染，根除幽门螺杆菌可以大大降低复发率。检查了80例急性十二指肠溃疡穿孔患者 (平均年龄 = 52岁，范围17-85) 的幽门螺杆菌患病率，并与年龄和性别匹配的医院对照患者进行了比较。通过血清抗幽门螺杆菌IgG (helico-g试剂盒，Porton) 评估幽门螺杆菌状态，使用18或更低的滴度为阴性，特异性为89%，敏感性为88%。只有47% 的十二指肠溃疡穿孔患者的幽门螺杆菌阳性，这与对照组的50% 值相似。在51例十二指肠溃疡穿孔患者中，还在手术后4-10周进行了14c-尿素呼吸测试，这证实只有49% 例幽门螺杆菌阳性。这些患者均未接受可能根除感染的围手术期药物。幽门螺杆菌阳性和幽门螺杆菌阴性的十二指肠溃疡穿孔患者在年龄 (53，51)，吸烟 (84%，83%) 和每周饮酒超过15单位 (42%，38%) 方面相似。只有24% 名幽门螺杆菌感染者和24% 名无幽门螺杆菌感染者在急性穿孔前被诊断出十二指肠溃疡。定期使用非甾体抗炎药 (NSAID) 在患有 (44%) 和没有 (45%) 幽门螺杆菌的患者中很常见。总之，急性十二指肠溃疡穿孔与幽门螺杆菌感染之间缺乏关联，这表明十二指肠溃疡穿孔与慢性十二指肠溃疡疾病的发病机理不同，不应将第一种疾病简单地视为第二种疾病的并发症。
发表日期： 1993 Oct
中文标题： 幽门螺杆菌: DNA指纹图谱的比较为家族性感染提供了证据。
论文作者： Bamford, K B; Bickley, J; Collins, J S; Johnston, B T; Potts, S; Boston, V; Owen, R J; Sloan, J M
英文摘要： Although a high prevalence of antibodies to Helicobacter pylori has been documented within families, culture and DNA typing of strains from infected children and their parents has not been evaluated. This study aimed to analyse H pylori infection within family groups. Endoscopy, gastric biopsy, and H pylori culture were performed on all eight parents of four children who presented with dyspepsia and who had a positive H pylori culture. All biopsy specimens were cultured on Columbia based blood agar under microaerophilic conditions for four days. The DNA from each strain was extracted and electrophoretic patterns were compared after digestion with restriction endonucleases Hae III or Hind III. Ribotyping using a biotinylated cDNA probe prepared from 16S and 23S rRNA of H pylori NCTC 11638 was also used. Seven of the parents were positive for H pylori on urease testing, histology, and on culture. DNA typing showed the same or a similar strain to be present in at least two family members in three of the four family groups. In family 1, the mother, father, and child all had an identical strain; in family 2, father and son had a similar related strain; father and mother had the same strain in family 3; and all strains were unique in family 4. These data provide evidence for either intrafamilial cross infection or a common source of infection within family groups.Images: Figure 1Figure 2
中文摘要： 尽管已记录家庭中幽门螺杆菌抗体的患病率很高，但尚未评估感染儿童及其父母的菌株的培养和DNA分型。本研究旨在分析家庭群体内的幽门螺杆菌感染情况。对四名消化不良且幽门螺杆菌培养阳性的儿童的所有八名父母进行了内窥镜检查，胃活检和幽门螺杆菌培养。将所有活检标本在微需氧条件下在基于Columbia的血琼脂上培养4天。提取每个菌株的DNA，并用限制性核酸内切酶Hae III或Hind III消化后比较电泳图谱。还使用了使用从幽门螺杆菌NCTC 11638的16s和23srrna制备的生物素化cDNA探针的核糖分型。在尿素酶测试，组织学和培养中，有7名父母对幽门螺杆菌呈阳性。DNA分型显示，在四个家族中的三个家族中，至少两个家族成员中存在相同或相似的菌株。在家庭1中，母亲、父亲和孩子都有相同的菌株; 在家庭2中，父亲和儿子有相似的相关菌株; 在家庭3中，父亲和母亲有相同的菌株；并且所有菌株在家族4中都是独特的。这些数据为家族内交叉感染或家族群体内的共同传染源提供了证据。图像: 图1图2
发表日期： 1993 Oct
中文标题： 对有胃肠道症状的HIV阳性患者的内镜监测活检标本的审核。
论文作者： Lim, S G; Lipman, M C; Squire, S; Pillay, D; Gillespie, S; Sankey, E A; Dhillon, A P; Johnson, M A; Lee, C A; Pounder, R E
英文摘要： An audit of upper gastrointestinal endoscopy in HIV infected patients with gastrointestinal symptoms assessed the frequency of disease detected by endoscopy and routine laboratory analysis of surveillance biopsy specimens. Sixty nine consecutive endoscopies were performed in 59 HIV infected patients. Endoscopic biopsy specimens were taken from the lower oesophagus, gastric antrum, and third part of the duodenum for virology, histopathology, parasitology, bacteriology, and mycobacterial culture. Endoscopic appearances detected disease in 25/59 (42.4%) patients (oesophageal candida, 14; oesophageal ulcer, 3; Kaposi's sarcoma, 4; others, 4), but only 4/43 (9.3%) specimens showed evidence of disease in the absence of endoscopic abnormality. Virology for cytomegalovirus (detection of early antigenic fluorescent foci and culture) was positive in 6/59 (10.2%) patients, but parasitology and mycobacterial culture were negative in all cases. Histopathology was abnormal in 11/52 (21%) oesophageal biopsy specimens, 13/47 (28%) gastric biopsy specimens, and 4/65 (6%) duodenal biopsy specimens. Abnormal findings were found predominantly in those with advanced HIV disease (CDC Stage IV) (21/33 patients (64%)) compared with those with early HIV disease (CDC Stage II) (5/26 (19%)). In conclusion, upper gastrointestinal endoscopy detects macroscopic disease in AIDS patients and those with low CD4 counts, but routine surveillance biopsy specimens of apparently normal bowel in early HIV disease (or where CD4 counts are greater than 0.2 x 10(9)/1) are of little value.
中文摘要： 对患有胃肠道症状的HIV感染患者进行上消化道内窥镜检查的审核评估了通过内窥镜检查和常规实验室监测活检标本分析发现的疾病频率。对59例HIV感染患者进行了69次连续内窥镜检查。内窥镜活检标本取自食管下部，胃窦和十二指肠的第三部分，用于病毒学，组织病理学，寄生虫学，细菌学和分枝杆菌培养。内镜检查发现25/59例 (42.4%) 患者疾病 (食管念珠菌，14例; 食管溃疡，3例; 卡波济肉瘤，4例; 其他，4例)，但只有4/43例 (9.3%) 标本在没有内镜异常的情况下显示出疾病的证据。6/59例 (10.2% 例) 患者的巨细胞病毒病毒学 (早期抗原荧光灶的检测和培养) 阳性，但所有病例的寄生虫学和分枝杆菌培养均为阴性。在11/52 (21%) 食管活检标本，13/47 (28%) 胃活检标本和4/65 (6%) 十二指肠活检标本中，组织病理学异常。与早期HIV疾病 (CDC II期) (5/26 (19%)) 相比，异常发现主要发生在晚期HIV疾病 (CDC IV期) (21/33例 (64%))。总之，上消化道内窥镜检查可在AIDS患者和CD4计数低的患者中发现肉眼可见的疾病，但在早期HIV疾病中 (或CD4计数大于0.2x10(9)/1) 的常规监测活检标本价值不大。
发表日期： 1993 Oct
英文标题： Serological diagnosis of Helicobacter pylori.[全文]
中文标题： 幽门螺杆菌的血清学诊断。
论文作者： Blecker, U; Vandenplas, Y
英文摘要：
中文摘要：
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