(有关HP文献开始于1987年,这里将按年份陆续整理成库以窥全貌)
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发表日期: 1993-12-17
英文标题: Transmission of Helicobacter pylori via faeces.
中文标题: 幽门螺杆菌通过粪便传播。
论文作者: Leverstein-van Hall MA, van der Ende A, van Milligen de Wit M, Tytgat GN, Dankert J.
英文摘要:
中文摘要:
发表日期: 1993-12-03
英文标题: [Helicobacter pylori infection and peptic ulcer].
中文标题: 。
论文作者: Jaspersen D, Hammar CH.
英文摘要:
中文摘要:
发表日期: 1993-12-01
英文标题: [Anti-Hp antibody may vary with strain used as antigen possibly due to serological diversity].
中文标题: [可能由于血清学多样性,抗Hp抗体可能因用作抗原的菌株而异]。
论文作者: Mizuta T(1), Inoue H, Hayashi T, Shimoyama T.
英文摘要: Western blotting and ELISA techniques have been used to investigate antigen specificity of systemic responses. Immunoblotting studies demonstrate several major protein antigens that are detected most sera. H. pylori immunoblotting studies using rabbit hyperimmune sera identify several distinct strains. Seven monoclonal antibodies which are prepared in our laboratory recognize 33-35 kDa and 66 kDa of H. pylori. ELISA studies using the monoclonal antibody reveals considerable antigenic diversity among H. pylori strains. Serotyping of clinically isolated H. pylori Seems to be useful in clarifying the etiological roles of this bacteria. In spite of studies by several investigators, serotyping of H. pylori has not been established yet. Our studies suggest that H. pylori need to be further subdivided serologically.
中文摘要: 蛋白质印迹和ELISA技术已被用于研究全身反应的抗原特异性。。H、 使用兔超免疫血清的幽门螺杆菌免疫印迹研究鉴定了几种不同的菌株。我们实验室制备的七种单克隆抗体可识别33-35 kDa和66 kDa的幽门螺杆菌。使用单克隆抗体的ELISA研究揭示了幽门螺杆菌菌株之间相当大的抗原多样性。临床分离的幽门螺杆菌的血清分型似乎有助于阐明这种细菌的病因作用。尽管几位研究人员进行了研究,但幽门螺杆菌的血清分型尚未建立。我们的研究表明,幽门螺杆菌需要在血清学上进一步细分。
发表日期: 1993-12-01
英文标题: Inhibition of gastric mucosal mucin receptor by Helicobacter pylori lipopolysaccharide.
中文标题: 幽门螺杆菌脂多糖对胃粘膜粘蛋白受体的抑制作用。
论文作者: Piotrowski J(1), Slomiany A, Slomiany BL.
英文摘要: A gastric mucosal mucin receptor has been isolated from the epithelial cell membrane by affinity chromatography on wheat germ agglutinin. The receptor protein displayed an apparent molecular weight of 97kDa and exhibited binding specific to gastric mucin in a concentration-dependent manner. The binding of mucin to the mucosal receptor was inhibited by lipopolysaccharide from H. pylori. The inhibitory effect was proportional to the concentration of lipopolysaccharide up to 30 micrograms/ml, at which concentration a 91% decrease in binding occurred. The results suggest that H. pylori lipopolysaccharide is capable of disrupting the integrity of mucus perimeter of gastric mucosal defense by interfering with epithelial cell-mucin binding.
中文摘要: 通过小麦胚芽凝集素亲和层析从上皮细胞膜上分离出胃粘膜粘蛋白受体。受体蛋白的表观分子量为97kDa,并以浓度依赖性方式表现出对胃粘蛋白的特异性结合。幽门螺杆菌的脂多糖抑制粘蛋白与粘膜受体的结合。抑制作用与脂多糖浓度成正比,高达30微克/毫升,在该浓度下结合减少91%。结果表明,幽门螺杆菌脂多糖能够通过干扰上皮细胞粘蛋白结合来破坏胃粘膜防御粘液周长的完整性。
发表日期: 1993-12-01
英文标题: [Polymorphism of Helicobacter pylori urease genes and gastric diseases].
中文标题: [幽门螺杆菌尿素酶基因多态性与胃病]。
论文作者: Sugiyama T(1), Awakawa T.
英文摘要: Helicobacter pylori (HP) is now recognized as a major etiologic agent of chronic gastritis and peptic ulcer and is recently presumed to be a cofactor in the occurrence of gastric cancer. HP has a high urease activity, which possibly plays a role in the pathogenesis of gastroduodenal diseases. In this paper, the function and the genetic structure of HP urease gene and the restriction fragment length polymorphism (RFLP) among HP strains using a probe of HP urease alpha subunit gene were described. HP urease gene cluster, which included structure genes, regulatory genes and accessory genes was identified and associated with the activity and the assembly. The RFLP pattern of HP tends to be distinct in gastric diseases, 460 bp pattern in gastric ulcer and 1,100 bp pattern in chronic gastritis, by Hae III digestion. HP showing a 460 bp RFLP pattern possessed a relatively high urease activity.
中文摘要: 幽门螺杆菌(HP)现在被认为是慢性胃炎和消化性溃疡的主要病原体,最近被认为是胃癌发生的辅助因素。HP具有高尿素酶活性,可能在胃十二指肠疾病的发病机制中起作用。本文描述了HP尿素酶基因的功能和遗传结构,以及利用HP尿素酶α亚基基因探针对HP菌株进行限制性片段长度多态性(RFLP)分析。鉴定了HP脲酶基因簇,包括结构基因,调控基因和辅助基因,并与活性和组装相关。通过Hae III消化,HP的RFLP模式在胃病中趋于不同,在胃溃疡中为460 bp,在慢性胃炎中为1100 bp。显示460 bp RFLP模式的HP具有相对较高的脲酶活性。
发表日期: 1993-12-01
中文标题: 幽门螺杆菌相关性慢性胃炎治疗前后的局部和全身免疫反应。
论文作者: Engstrand L(1), Gustavsson S, Schwan A, Scheynius A.
英文摘要: Ten patients with Helicobacter pylori-associated chronic gastritis were given combination therapy for 6 weeks with a bismuth subnitrate-containing compound and bacampicillin. The eradication rate was 40% 6 weeks after the end of treatment. Two patients remained H. pylori-negative at long-term follow-up after 6 and 17 months; that is, H. pylori was only eradicated in 20% of the patients after long-term observation. By dot blot and immunoblotting both urease and an urease-associated heat shock protein (HSP62) were found to be specific and constant immunodominant H. pylori antigens. The immunohistologic pattern showed induced expression of HLA-DR and HSP62, but not of ICAM-1, in all but two biopsy specimens of gastric epithelial cells. This study suggests i) that long-term observation is important when evaluating the efficacy of anti-H. pylori therapy; ii) that the immune defense mechanisms in the gastric mucosa differ from those in inflammatory conditions affecting other organs, where ICAM-1 and HLA-DR seem to be governed by a common regulator; and iii) that the immunopathologic effects of H. pylori may be caused by autologous and/or bacterial HSPs, which act as triggering factors in the development and persistence of the chronic inflammation in the gastric mucosa.
中文摘要: 10例幽门螺杆菌相关性慢性胃炎患者接受含亚硝酸铋化合物和巴氨苄青霉素的联合治疗6周。治疗结束后6周根除率为40%。;也就是说,经过长期观察,只有20%的患者根除了幽门螺杆菌。通过斑点印迹和免疫印迹,发现脲酶和脲酶相关热休克蛋白(HSP62)都是特异性和恒定的免疫显性幽门螺杆菌抗原。免疫组织学模式显示,除两个胃上皮细胞活检标本外,所有标本均诱导HLA-DR和HSP62表达,但不诱导ICAM-1表达。这项研究表明i)在评估抗幽门螺杆菌治疗的疗效时,长期观察很重要;ii)胃粘膜中的免疫防御机制不同于影响其他器官的炎症状态,其中ICAM-1和HLA-DR似乎由共同的调节剂控制;iii)幽门螺杆菌的免疫病理作用可能是由自体和/或细菌HSP引起的,这些HSP是胃粘膜慢性炎症发展和持续存在的触发因素。
发表日期: 1993-12-01
英文标题: Helicobacter pylori prevalence in northeastern Thailand.
中文标题: 泰国东北部幽门螺杆菌患病率。
论文作者: Chinprasatsak S(1), Wilairatana P, Visalwadi P, Sanguansri P, Batara L, Kityaporn D, Looareesuwan S, Kurathong S, Charoenlarp P.
英文摘要: Helicobacter pylori is distributed worldwide and has been demonstrated in Thailand. However, no study has been conducted so far in northeastern Thailand. The objective of this study was to find the prevalence of H. pylori in patients undergoing upper gastrointestinal endoscopy in northeastern part of Thailand. One hundred and twenty-six patients undergoing surgery between November 1992 and January 1993 were studied. Biopsies were done at antrum, corpus, and other positive lesions. Diagnostic tests of H. pylori by using CLO test, microbiological tests (Gram stain and culture), and histological examination (hematoxylin and eosin) were carried out. The prevalence of H. pylori by CLO test, Gram stain, culture, and histology were 49.2%, 61.9%, 22.2%, and 45.2% respectively. The overall prevalence of H. pylori by all diagnostic tests was 73.8% (95% confidence interval = 66.1-81.5%). This study revealed a high prevalence rate of H. pylori in patients which should alert clinicians who practice in this geographical area.
中文摘要: 幽门螺杆菌在世界范围内分布,并已在泰国得到证实。然而,到目前为止,泰国东北部尚未进行任何研究。这项研究的目的是发现泰国东北部接受上消化道内镜检查的患者中幽门螺杆菌的患病率。对1992年11月至1993年1月期间接受手术的126例患者进行了研究。在胃窦,胃体和其他阳性病变处进行活检。通过使用CLO测试,微生物测试(革兰氏染色和培养)和组织学检查(苏木精和曙红)进行幽门螺杆菌的诊断测试。通过CLO测试,革兰氏染色,培养和组织学检测,幽门螺杆菌的患病率分别为49.2%,61.9%,22.2%和45.2%。所有诊断测试中幽门螺杆菌的总体患病率为73.8%(95%置信区间=66.1-81.5%)。这项研究显示,患者中幽门螺杆菌的患病率很高,这应该提醒在这个地理区域执业的临床医生。
发表日期: 1993-12-01
英文标题: [Exudative enteropathy and gastritis caused by Helicobacter pylori in children].
中文标题: [儿童幽门螺杆菌引起的渗出性肠病和胃炎]。
论文作者: Maherzi A, Ben Jilani S, Fendri C, Meknini B, Bousnina S.
英文摘要:
中文摘要:
发表日期: 1993-12-01
英文标题: Helicobacter pylori: a primer for 1994.
中文标题: 幽门螺杆菌:1994年的引物。
论文作者: Marshall BJ(1).
英文摘要: Ten years after the original isolation of Helicobacter pylori, we know that this interesting new bacterium infects half of the world's population and at least 25% of people in the United States. The mode of transmission is probably fecal-oral, and its prevalence increases in lower socioeconomic groups or older people. Fortunately, the organism can now be diagnosed using serology, breath testing, biopsy, and culture. We also have effective means of therapy in the United States giving 80 to 95% cure rates in 14 days. In the past four years at least five well-controlled, double-blind studies have shown that curing Helicobacter pylori usually results in curing duodenal ulcer disease. Increasing data confirm that this also holds true for gastric-ulcer and ulcer complications. The role of Helicobacter pylori in non-ulcer dyspepsia is still controversial, and its association with increased gastric-cancer risk offers an exciting opportunity for further research.
中文摘要: 最初分离出幽门螺杆菌十年后,我们知道这种有趣的新细菌感染了世界一半的人口和至少25%的美国人口。传播方式可能是粪便-口腔传播,其患病率在较低的社会经济群体或老年人中增加。幸运的是,现在可以使用血清学,呼吸测试,活检和培养来诊断该生物体。我们在美国也有有效的治疗手段,在14天内治愈率达到80%至95%。在过去的四年中,至少有五项控制良好的双盲研究表明,治愈幽门螺杆菌通常可以治愈十二指肠溃疡病。越来越多的数据证实,这也适用于胃溃疡和溃疡并发症。幽门螺杆菌在非溃疡性消化不良中的作用仍存在争议,其与胃癌风险增加的关联为进一步研究提供了令人兴奋的机会。
发表日期: 1993-12-01
英文标题: Diarrhoeal disease: current concepts and future challenges. Malnutrition and infection.
中文标题: 腹泻病:当前的概念和未来的挑战。营养不良和感染。
论文作者: Walker-Smith JA(1).
英文摘要: In individual children in developed countries it is possible to follow a sequence of infection of the gastrointestinal tract leading to chronic diarrhoea which, if it long persists, may in turn lead to undernutrition. Both in individuals and epidemiologically in developing countries it is, by contrast, often difficult to be certain whether infection precedes under-nutrition or vice versa. Chronic diarrhoea is heterogeneous and aetiology varies from community to community. Unlike acute diarrhoea, for which there is highly effective unitary therapy (oral rehydration therapy), diverse therapies are required for chronic diarrhoea based on specific diagnoses and so render community strategies difficult. The importance of adequate calorie intake is emphasized but when there is intolerance to food ingested this is counter-productive. The relative importance of post-infective food intolerance remains controversial and in many communities is unknown. Increasing emphasis is now given to the role of infection in pathogenesis of diarrhoea and malnutrition, e.g. the acquired immune deficiency syndrome and Helicobacter pylori. Hospital-based case studies including small intestinal biopsy in individual communities, by virtue of an 'iceberg effect', may prove useful for insights into aetiology and pave the way for interventions, e.g. antibiotics or dietary therapy.
中文摘要: 在发达国家的个别儿童中,可能会发生一系列胃肠道感染,导致慢性腹泻,如果长期持续,可能反过来导致营养不良。相比之下,无论是在个人还是在发展中国家的流行病学上,通常很难确定感染是在营养不良之前还是在营养不良之前。慢性腹泻是多种多样的,病因因社区而异。与急性腹泻不同,急性腹泻有高效的单一疗法(口服补液疗法),慢性腹泻需要根据具体诊断进行多种疗法,因此难以采取社区策略。人们强调了摄入足够热量的重要性,但如果对摄入的食物不耐受,则会适得其反。感染后食物不耐受的相对重要性仍然存在争议,在许多社区尚不清楚。现在越来越重视感染在腹泻和营养不良发病机制中的作用,例如获得性免疫缺陷综合征和幽门螺杆菌。基于医院的案例研究,包括个别社区的小肠活检,凭借“冰山效应”,可能有助于深入了解病因,并为干预措施铺平道路,例如抗生素或饮食疗法。
发表日期: 1993-12-01
中文标题: 早期和晚期胃腺癌中的幽门螺杆菌感染:143名台湾患者的血清流行病学研究。
论文作者: Lin JT(1), Wang JT, Wang TH, Wu MS, Chen CJ.
英文摘要: The association between gastric adenocarcinoma and Helicobacter pylori infection remains controversial. A seroprevalence study of Helicobacter pylori infection in 143 patients with gastric adenocarcinoma and a control group of 823 subjects randomly selected from four areas in Taiwan, was carried out to elucidate the association. The overall seropositivity of Helicobacter pylori in gastric adenocarcinoma patients (62.9%) was higher than in controls (54.4%), but the difference was not statistically significant (p > 0.05). The seropositivity of early gastric adenocarcinoma (61.5%) was not significantly different from that of advanced gastric adenocarcinoma (63.2%). Various demographic factors such as sex, blood type, cigarette smoking, tumor histology and location were not associated with the seroprevalence of Helicobacter pylori. The age-specific seroprevalence of Helicobacter pylori tended to be higher in younger patients and decreased after the age of 60 years in gastric adenocarcinoma, in contrast to a stepwise increase of seropositivity in controls. This suggests that Helicobacter pylori infection in early life may be a contributory factor in gastric carcinogenesis.
中文摘要: 胃腺癌与幽门螺杆菌感染之间的关联仍存在争议。从台湾四个地区随机抽取143例胃腺癌患者和823名受试者作为对照组,进行了幽门螺杆菌感染的血清流行病学研究,以阐明这种关联。胃腺癌患者幽门螺杆菌总阳性率(62.9%)高于对照组(54.4%),但差异无统计学意义(p>0.05)。早期胃腺癌(61.5%)与晚期胃腺癌(63.2%)的血清阳性率无显着差异。各种人口统计学因素,如性别,血型,吸烟,肿瘤组织学和位置与幽门螺杆菌的血清阳性率无关。幽门螺杆菌的年龄特异性血清阳性率在年轻患者中往往较高,在胃腺癌中60岁后下降,而对照组血清阳性率逐步增加。这表明早期幽门螺杆菌感染可能是胃癌发生的一个促成因素。
发表日期: 1993-12-01
英文标题: Glucose utilization and lactate production by Helicobacter pylori.
中文标题: 。
论文作者: Mendz GL(1), Hazell SL, Burns BP.
英文摘要: The transport and incorporation of D-glucose into the human pathogen Helicobacter pylori was investigated employing radioactive tracer analysis and 1H and 13C nuclear magnetic resonance spectroscopy. The bacterium was found to utilize D-glucose contrary to the accepted view that it cannot catabolize carbohydrates. Under the experimental conditions employed, the rate of transport of [14C]glucose was 3.24 mmol min-1 (g protein)-1, and the rate of incorporation into the cellular mass was 1.06 mumol h-1 (g protein)-1. The utilization of [13C]glucose showed biphasic characteristics with a slower initial period followed by a phase with a rate of utilization at least an order of magnitude faster. The apparent rates of decline of glucose levels during both phases varied between strains and depended on the growth conditions of the bacteria prior to harvesting. The main product of glucose catabolism was identified as lactate. These findings provide new perspectives into the physiology of H. pylori and have implications for the active search to develop appropriate therapies for the micro-organism.
中文摘要: 使用放射性示踪剂分析和1H和13C核磁共振波谱研究了D-葡萄糖向人类病原体幽门螺杆菌的转运和掺入。发现该细菌利用D-葡萄糖,这与公认的观点相反,即它不能分解碳水化合物。在所采用的实验条件下,[14C]葡萄糖的转运速率为3.24 mmol min-1(g蛋白)-1,掺入细胞团的速率为1.06 mumol h-1(g蛋白)-1。[13C]葡萄糖的利用表现出双相特征,初始阶段较慢,然后是利用率至少快一个数量级的阶段。两个阶段葡萄糖水平的表观下降率因菌株而异,并取决于收获前细菌的生长条件。葡萄糖分解代谢的主要产物被鉴定为乳酸。这些发现为幽门螺杆菌的生理学提供了新的视角,并对积极寻找开发适当的微生物疗法具有重要意义。
发表日期: 1993-12-01
英文标题: [Nested-polymerase chain reaction in detection of Helicobacter pylori in human dental plaque].
中文标题: [巢式聚合酶链反应检测人牙菌斑中的幽门螺杆菌]。
论文作者: Yang HT(1).
英文摘要: A nested polymerase chain reaction (N-PCR) for the specific detection of Helicobacter pylori (H. pylori) was developed with two primer pairs derived from the urease gene A of H. pylori. The N-PCR was used to detect all 21 H. pylori strains, including 20 clinical isolates and 1 reference strain (NCTC 14126), but failed to detect 12 other bacterial species. It was shown that the PCR assay is 100% specific. Tenfold serial dilution experiments revealed that N-PCR could detect as little as 0.1 fg DNA. Samples of dental plaque and stomach biopsies from 29 patients were collected. Gastric specimens were tested by N-PCR, and the results were compared with those of culture, urease test and histological examination (reference standard). H. pylori sequences were detected by PCR showing that 21 were positive. The dental plaque samples from 8 of the 21 patients who tasted positive of gastric biopsies were positive by N-PCR. However, none of 8 dental plaque samples from the patients whose gastric samples were negative showed amplification. Dual therapy was given to two patients who were H. pylori positive in gastric mucosa and dental plaque. One month after treatment, H. pylori was eradicated from the gastric mucosa, but persisted in dental plaque.
中文摘要: 利用幽门螺杆菌尿素酶基因A的两对引物,建立了特异性检测幽门螺杆菌(H.pylori)的巢式聚合酶链反应(N-PCR)。N-PCR检测了21株幽门螺杆菌,包括20株临床分离株和1株参考菌株(NCTC 14126),但未检测到其他12种细菌。结果表明,PCR检测具有100%的特异性。十倍连续稀释实验表明,N-PCR可以检测到少至0.1 fg DNA。收集了29例患者的牙菌斑和胃活检样本。通过N-PCR检测胃标本,并将结果与培养,尿素酶试验和组织学检查(参考标准)进行比较。H、 通过PCR检测幽门螺杆菌序列,显示21个阳性。21例胃活检呈阳性的患者中有8例的牙菌斑样本通过N-PCR呈阳性。然而,来自胃样本阴性的患者的8个牙菌斑样本中没有一个显示扩增。对两名胃粘膜和牙菌斑中幽门螺杆菌阳性的患者进行了双重治疗。治疗一个月后,幽门螺杆菌从胃粘膜中被根除,但持续存在于牙菌斑中。
发表日期: 1993-12-01
英文标题: [Therapeutic reflections and strategies on the topic of Helicobacter pylori: what, who, why?].
中文标题: [关于幽门螺杆菌主题的治疗反思和策略:什么,谁,为什么?]。
论文作者: Peyre S, Bologna E, Sategna-Guidetti C.
英文摘要:
中文摘要:
发表日期: 1993-12-01
英文标题: The immunological aspects of chronic gastritis.
中文标题: 慢性胃炎的免疫学方面。
论文作者: Sugiyama T(1), Yachi A.
英文摘要:
中文摘要:
发表日期: 1993-12-01
英文标题: Purification of Helicobacter pylori superoxide dismutase and cloning and sequencing of the gene.
中文标题: 幽门螺杆菌超氧化物歧化酶的纯化及其基因的克隆和测序。
论文作者: Spiegelhalder C(1), Gerstenecker B, Kersten A, Schiltz E, Kist M.
英文摘要: The superoxide dismutase (SOD) of Helicobacter pylori, a pathogenic bacterium which colonizes the gastric mucosa, evoking a marked inflammatory response, was purified and characterized, and the N-terminal amino acid sequence was determined. The enzyme consists of two identical subunits each with an apparent molecular weight of 24,000. Analysis of the primary structure and inhibition studies revealed that H. pylori possesses a typical procaryotic iron-containing enzyme. No other isoenzymes could be detected. Indirect gold immunostaining of H. pylori SOD with a polyclonal antibody directed against the iron-containing SOD of Escherichia coli showed a surface-associated localization of the enzyme. The H. pylori SOD gene was cloned by functional complementation of a SOD-deficient E. coli mutant. Sequencing and alignment revealed striking homology to the following facultative intracellular human pathogens: Listeria ivanovii, Listeria monocytogenes, Coxiella burnetti, Porphyromonas gingivalis, Legionella pneumophila, and Entamoeba histolytica. An open reading frame of 642 bp encoding 214 amino acids was determined. There was no leader sequence detectable. Cloning of the H. pylori SOD gene is one of the prerequisites to investigation of its pathophysiological role in the defense against antimicrobial mechanisms of polymorphonuclear granulocytes.
中文摘要: 纯化并鉴定了幽门螺杆菌(Helicobacter pylori)的超氧化物歧化酶(SOD),幽门螺杆菌是一种定殖于胃粘膜,引起明显炎症反应的致病细菌,并测定了其N端氨基酸序列。该酶由两个相同的亚基组成,每个亚基的表观分子量为24000。对一级结构和抑制研究的分析表明,幽门螺杆菌具有典型的原核含铁酶。没有检测到其他同工酶。用针对大肠杆菌含铁SOD的多克隆抗体对幽门螺杆菌SOD进行间接金免疫染色,显示该酶的表面相关定位。通过SOD缺陷型大肠杆菌突变体的功能互补克隆了幽门螺杆菌SOD基因。测序和比对显示与以下兼性细胞内人类病原体具有惊人的同源性:伊凡诺维奇李斯特菌,单核细胞增生李斯特菌,伯内蒂柯克斯体,牙龈卟啉单胞菌,嗜肺军团菌和溶组织内阿米巴。确定了编码214个氨基酸的642 bp的开放阅读框。没有检测到前导序列。幽门螺杆菌SOD基因的克隆是研究其在防御多形核粒细胞抗菌机制中的病理生理作用的先决条件之一。
发表日期: 1993-12-01
中文标题: 发展中国家幽门螺杆菌感染的诊断:两种ELISA的比较和血清流行病学研究。
论文作者: Bodhidatta L(1), Hoge CW, Churnratanakul S, Nirdnoy W, Sampathanukul P, Tungtaem C, Raktham S, Smith CD, Echeverria P.
英文摘要: Serology to detect antibodies to Helicobacter pylori is not frequently used as a diagnostic tool in developing countries. When compared to a commercial ELISA, an ELISA constructed and validated in Thailand had a higher sensitivity (98% vs. 85%), specificity (76% vs. 66%), and negative predictive value (97% vs. 76%) for the detection of H. pylori infection among 104 patients with dyspepsia evaluated by endoscopy. The positive predictive value was 88% for both tests. Serum antibody levels fell significantly 5-8 months after eradication of infection in 8 Thai patients (P = .009). By 8 years of age, > 50% of Thai persons living in urban and rural locations were seropositive. The low negative predictive value of the commercial ELISA limits the usefulness of this assay as a diagnostic tool in Thailand and suggests a need to reevaluate H. pylori serologic tests when used in populations living in developing countries.
中文摘要: 在发展中国家,检测幽门螺杆菌抗体的血清学不常被用作诊断工具。与商业ELISA相比,在泰国构建和验证的ELISA具有更高的敏感性(98%比85%),特异性(76%比66%)和阴性预测值(97%比76%),用于检测104例消化不良患者的幽门螺杆菌感染通过内窥镜检查评估。两种测试的阳性预测值均为88%。8名泰国患者在根除感染后5-8个月血清抗体水平显着下降(P=0.009)。到8岁时,居住在城市和农村地区的泰国人中有50%以上是血清阳性的。商业ELISA的低阴性预测值限制了该测定法在泰国作为诊断工具的实用性,并表明在发展中国家人群中使用幽门螺杆菌血清学检测时需要重新评估。
发表日期: 1993-12-01
中文标题: 正常人和十二指肠溃疡患者的酸分泌和血清胃泌素:幽门螺杆菌的作用。
论文作者: Peterson WL(1), Barnett CC, Evans DJ Jr, Feldman M, Carmody T, Richardson C, Walsh J, Graham DY.
英文摘要: OBJECTIVES: To compare gastric secretory function in patients with duodenal ulcer and in healthy volunteers with and without Helicobacter pylori infection.METHODS: Basal acid output, peak acid output, meal-stimulated acid output, fasting and meal-stimulated serum gastrin concentrations were measured in 136 healthy volunteers (63 H. pylori positive, 73 H. pylori negative) and 52 duodenal ulcer patients, all but one of whom were H. pylori positive.RESULTS: By multivariate linear regression analysis, H. pylori infection was a significant negative predictor of basal acid output and a positive predictor of fasting and meal-stimulated gastrin concentrations. When compared to truly normal (i.e., H. pylori-negative) control subjects, duodenal ulcer patients had elevated basal acid output, peak acid output, fasting and meal-stimulated gastrin concentrations.CONCLUSIONS: Our results show that in patients with duodenal ulcer disease, hypergastrinemia is largely related to gastric H. pylori infection, whereas acid hypersecretion is due to factors other than H. pylori.
中文摘要: 目的:比较十二指肠溃疡患者和有无幽门螺杆菌感染的健康志愿者的胃分泌功能。方法:测定136名健康志愿者(63名幽门螺杆菌阳性,73名幽门螺杆菌阴性)和52名十二指肠溃疡患者的基础酸输出量,峰值酸输出量,膳食刺激酸输出量,空腹和膳食刺激血清胃泌素浓度,其中除一名幽门螺杆菌阳性外。结果:通过多元线性回归分析,幽门螺杆菌感染是基础酸输出的显着负预测因子,也是空腹和膳食刺激胃泌素浓度的阳性预测因子。与真正正常(即幽门螺杆菌阴性)的对照受试者相比,十二指肠溃疡患者的基础酸输出量,峰值酸输出量,禁食和膳食刺激的胃泌素浓度升高。结论:我们的研究结果表明,在十二指肠溃疡患者中,高胃泌素血症与胃幽门螺杆菌感染密切相关,而酸分泌过多是由幽门螺杆菌以外的因素引起的。
发表日期: 1993-12-01
英文标题: Gastric ammonia has a potent ulcerogenic action on the rat stomach.
中文标题: 胃氨对大鼠胃具有强烈的致溃疡作用。
论文作者: Murakami M(1), Saita H, Teramura S, Dekigai H, Asagoe K, Kusaka S, Kita T.
英文摘要: BACKGROUND: The pathophysiological mechanism by which Helicobacter pylori induces mucosal injury has not been clarified. The aim of this study was to investigate the role of urea, urease, and ammonia in rat gastric mucosal lesions using an ex vivo chamber model.METHODS: Two groups of rats, normotensive rats and those subjected to ischemia, were studied. The gastric mucosa was examined histologically and macroscopically, and the transmucosal potential difference was measured.RESULTS: Instillation of urea into the stomach generated ammonia in the presence of urease. The amount of ammonia was increased depending on the concentration of urea and was closely associated with the severity of the histological lesions. The exposure of the stomach to 15-60 mmol/L ammonium hydroxide induced both a reduction in transmucosal potential difference and microscopic damage to the gastric mucosa in normotensive rats. Moreover, 15-60 mmol/L ammonium hydroxide produced severe macroscopic gastric lesions in the rats subjected to ischemia.CONCLUSIONS: These results show that ammonia is deleterious to the gastric mucosa and suggest the importance of urea, urease, and ammonia in the pathophysiology of gastric diseases in H. pylori-infected patients.
中文摘要: 背景:幽门螺杆菌诱导粘膜损伤的病理生理机制尚未阐明。本研究的目的是使用离体室模型研究尿素,脲酶和氨在大鼠胃粘膜病变中的作用。方法:研究两组大鼠,血压正常大鼠和缺血大鼠。对胃粘膜进行组织学和宏观检查,并测量跨粘膜电位差。结果:在脲酶存在下,将尿素滴入胃中产生氨。氨的量取决于尿素的浓度而增加,并且与组织学病变的严重程度密切相关。将胃暴露于15-60 mmol/L氢氧化铵诱导正常血压大鼠的跨粘膜电位差降低和胃粘膜的微观损伤。此外,15-60 mmol/L氢氧化铵在缺血大鼠中产生严重的肉眼可见的胃损伤。结论:这些结果表明氨对胃粘膜有害,并提示尿素,尿素酶和氨在幽门螺杆菌感染患者的胃病病理生理学中的重要性。
发表日期: 1993-12-01
中文标题: 有和没有幽门螺杆菌感染的慢性胃窦胃炎的不同消化间期胃窦十二指肠运动模式。
论文作者: Testoni PA(1), Bagnolo F, Masci E, Colombo E, Tittobello A.
英文摘要: Fasting antroduodenal motor activity was studied in 15 dyspeptic patients with chronic superficial antral gastritis and Helicobacter pylori infection (group A), 10 dyspeptic patients with chronic superficial antral gastritis without Helicobacter pylori infection (group B), and eight healthy control subjects (group C) by manometric recording of phases of the interdigestive migrating motor complex (MMC) prolonged over 240 min. A significantly lower incidence of activity fronts (phase III of MMC) starting from the antrum was observed in patients with gastritis and Helicobacter pylori infection vs patients without bacterial colonization (P = 0.013) and in these latter vs control subjects (P = 0.013). Likewise, the overall number of activity fronts was smaller in patients with gastritis than in healthy subjects (P = 0.034). Symptomatic evaluation was performed in the two groups of dyspeptic patients, without detecting any differences in frequency and severity of complaints. Our results show a significant reduction in the occurrence of interdigestive antral phase III of MMC in chronic gastritis associated with Helicobacter pylori infection, suggesting a possible relationship between fasting motility and bacterial colonization.
中文摘要: 对15例慢性浅表性胃窦胃炎和幽门螺杆菌感染的消化不良患者(A组),10例无幽门螺杆菌感染的慢性浅表性胃窦胃炎的消化不良患者(B组)和8例健康对照组(C组)的空腹胃窦运动活动进行了研究,通过测压记录消化间期移行运动复合体(MMC)的阶段延长超过240分钟。与无细菌定植的患者相比,胃炎和幽门螺杆菌感染的患者从胃窦开始的活动前沿(MMC的III期)的发生率显着降低(P=0.013),而后者与对照组相比(P=0.013).013)。同样,胃炎患者的活动前沿总数少于健康受试者(P=0.034)。对两组消化不良患者进行了症状评估,未发现投诉频率和严重程度有任何差异。我们的研究结果显示,与幽门螺杆菌感染相关的慢性胃炎中MMC消化间期胃窦III期的发生率显着降低,表明空腹运动与细菌定植之间可能存在关系。
发表日期: 1993-12-01
英文标题: The role of Helicobacter pylori in acid-peptic disease.
中文标题: 幽门螺杆菌在酸性消化性疾病中的作用。
论文作者: DeCross AJ(1), Marshall BJ.
英文摘要: Acid peptic disease is common, and its management is costly. Less than a decade ago, the traditional theories regarding the etiology and pathogenesis of acid peptic disease were upset by the discovery of Helicobacter pylori infection in association with chronic active gastritis. A substantial body of investigation after that discovery has established this infection as the major cause of human chronic active gastritis and has defined a critical role for H. pylori in the etiology, pathophysiology, and treatment of duodenal ulcer disease. Furthermore, evidence is accumulating to link H. pylori to gastric ulcers, non-ulcer dyspepsia, and even gastric carcinoma. Research has clarified some unique features of the organism that have been put to advantage in the development of diagnostic tests, and it has also clarified some features of the infection that make it difficult to treat. Although treatment is decidedly beneficial for certain patient subsets, simpler and more effective therapy is needed.
中文摘要: 酸性消化性疾病很常见,其管理费用高昂。不到十年前,关于酸性消化性疾病的病因和发病机制的传统理论因发现幽门螺杆菌感染与慢性活动性胃炎有关而受到破坏。这一发现后的大量研究已经确定这种感染是人类慢性活动性胃炎的主要原因,并确定了幽门螺杆菌在十二指肠溃疡病的病因,病理生理学和治疗中的关键作用。此外,越来越多的证据表明幽门螺杆菌与胃溃疡,非溃疡性消化不良甚至胃癌有关。研究已经阐明了该生物体的一些独特特征,这些特征在诊断测试的发展中发挥了优势,并且还阐明了感染的一些特征,这些特征使其难以治疗。虽然治疗对某些患者亚群无疑是有益的,但需要更简单和更有效的治疗。
发表日期: 1993-12-01
英文标题: The coccoid forms of Helicobacter pylori. Criteria for their viability.
中文标题: 幽门螺杆菌的球形。其生存标准。
论文作者: Bode G(1), Mauch F, Malfertheiner P.
英文摘要: The fact that Helicobacter pylori can revert to a coccoid form has stimulated speculation about its role in transmission and as a possible cause of reinfection in duodenal ulcer disease. Bismuth subcitrate (32 micrograms/ml), bismuth subsalicylate (64 micrograms/ml), amoxicillin (0.05 micrograms/ml) and erythromycin (4 micrograms/ml) inhibited the growth of H. pylori and stimulated the formation of basically respiring but non-culturable coccoid structures. The presence of polyphosphates as energy and phosphorus source permits a certain level of endogenous metabolism to preserve RNA and DNA, as well as structural components like cell wall, cell membrane and cytoplasma for at least 3 months. However, the applied standard laboratory methods were insufficient for regrowth of H. pylori out of the coccoid form.
中文摘要: 幽门螺杆菌可以恢复为球形的事实刺激了人们对其在传播中的作用以及作为十二指肠溃疡疾病再感染的可能原因的猜测。次柠檬酸铋(32微克/毫升),次水杨酸铋(64微克/毫升),阿莫西林(0.05微克/毫升)和红霉素(4微克/毫升)抑制幽门螺杆菌的生长,并刺激基本上呼吸但不可培养的球状结构的形成。多磷酸盐作为能量和磷源的存在允许一定水平的内源性代谢,以保存RNA和DNA,以及细胞壁,细胞膜和细胞质等结构成分至少3个月。然而,应用的标准实验室方法不足以使幽门螺杆菌从球形形式中再生。
发表日期: 1993-12-01
中文标题: 17个人群中3194名无症状受试者幽门螺杆菌感染的流行病学和危险因素。EUROGAST研究小组。
论文作者: [No authors listed]
英文摘要: The cross sectional study describes the prevalence of infection with Helicobacter pylori as determined by a serodiagnostic assay in over 3000 asymptomatic subjects, in two age groups 25-34 years and 55-64 years, from 17 geographically defined populations in Europe, North Africa, North America, and Japan, using a common protocol for blood collection and serological testing. In all populations combined, the prevalence of infection was higher in the older age group (62.4%) than in the younger age group (34.9%). There was no difference in prevalence of infection between men and women. Subjects with higher education had considerably lower levels of infection (34.1%) compared with subjects with education up to secondary level (46.9%) or those with primary education only (61.6%). This trend was confined to the older of the two age groups. In contrast a trend of increasing prevalence of infection with increasing body mass index was confined to the younger of the two age groups. There was no effect of smoking or alcohol consumption on the prevalence of infection after adjusting for the other risk factors. There was considerable variation in the prevalence of infection between the 17 populations but, within populations, low education standard was consistently and positively associated with the prevalence of infection.
中文摘要: 这项横断面研究描述了来自欧洲,北非,北美和日本17个地理定义人群的两个年龄组25-34岁和55-64岁的3000多名无症状受试者的血清诊断试验确定的幽门螺杆菌感染率,使用共同的采血和血清学检测方案。在所有人群中,老年组(62.4%)的感染率高于年轻组(34.9%)。男性和女性之间的感染率没有差异。与受过中等教育(46.9%)或仅受过初等教育(61.6%)的受试者相比,受过高等教育的受试者的感染水平要低得多(34.1%)。这种趋势仅限于两个年龄组中的老年人。相比之下,随着体重指数的增加,感染率增加的趋势仅限于两个年龄组中的年轻人。在调整了其他危险因素后,吸烟或饮酒对感染率没有影响。17个人群之间的感染率差异很大,但在人群中,低教育水平与感染率始终呈正相关。
发表日期: 1993-12-01
英文标题: When is Helicobacter pylori infection acquired?
中文标题: 何时获得幽门螺杆菌感染?
论文作者: Cullen DJ(1), Collins BJ, Christiansen KJ, Epis J, Warren JR, Surveyor I, Cullen KJ.
英文摘要: Cross sectional surveys have shown an increasing prevalence of Helicobacter pylori (H pylori) infection with increasing age in Western populations. The aim of this study was to examine the pattern of acquisition of H pylori infection over a 21 year period in a group of 141 adults who had blood samples and serum stored in 1969, 1978, and 1990. A prevalence of H pylori antibody of 39% in 1969 serum samples, 40.9% in 1978, and 34.8% in 1990 was found when assessed by an enzyme linked immunosorbent assay (ELISA). Of the 86 subjects who were seronegative in 1969, only six (7%) were seropositive in 1990. These data suggest that a cohort effect may contribute to the pattern of increasing prevalence of H pylori infection seen with increasing age. Acquisition of infection in adults is rare. It is unlikely, therefore, that reinfection will occur after successful eradication.
中文摘要: 横断面调查显示,随着西方人群年龄的增长,幽门螺杆菌(H pylori)感染的患病率越来越高。这项研究的目的是检查在1969年,1978年和1990年储存了血液样本和血清的141名成年人中21年内获得幽门螺杆菌感染的模式。通过酶联免疫吸附试验(ELISA)评估,1969年血清样本中幽门螺杆菌抗体的患病率为39%,1978年为40.9%,1990年为34.8%。在1969年血清阴性的86名受试者中,1990年只有6名(7%)血清阳性。这些数据表明,随着年龄的增长,队列效应可能有助于幽门螺杆菌感染率增加的模式。成人感染很少见。因此,成功根除后不太可能再感染。
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