(有关HP文献开始于1987年,这里将按年份陆续整理成库以窥全貌)
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发表日期: 1991-12-30
中文标题: 评估各种实验室技术诊断上消化道症状患者的幽门螺杆菌。
论文作者: Miller NM(1), Sathar MA, Naran AD, van den Ende J, Simjee AE, Manion G.
英文摘要: Helicobacter (Campylobacter) pylori is strongly associated with type B gastritis. The detection of H. pylori, which entails histological examination and culture of gastric biopsy specimens, takes several days. There has been much interest in developing more rapid tests, including non-invasive ones. Using histology and/or culture as the 'gold standard', several methods to detect H. pylori were compared and evaluated. The organism was detected in 84 of 100 consecutive patients attending the Gastrointestinal Unit of King Edward VIII Hospital for upper gastrointestinal tract endoscopy. Histological examination was the most sensitive (98%) and specific (100%) method used in detecting H. pylori in gastric biopsy specimens. An enzyme-linked immunosorbent assay to detect specific IgG antibodies to whole H. pylori organisms is a moderately sensitive (82%), non-invasive method but it is nonspecific (38%). Although culture was specific (100%), it was less sensitive (68%) than histological examination. The 'conventional' urease assays must be performed under controlled conditions (37 degrees C) for optimal results (sensitivity, 71%).
中文摘要: 幽门螺杆菌(弯曲杆菌)与B型胃炎密切相关。幽门螺杆菌的检测需要几天的时间,这需要组织学检查和胃活检标本的培养。人们对开发更快速的测试(包括非侵入性测试)非常感兴趣。使用组织学和/或培养作为“金标准”,比较和评估了几种检测幽门螺杆菌的方法。在爱德华八世国王医院胃肠科接受上消化道内窥镜检查的100名连续患者中,有84名患者检测到该生物体。组织学检查是检测胃活检标本中幽门螺杆菌最敏感(98%)和特异(100%)的方法。用于检测针对整个幽门螺杆菌生物体的特异性IgG抗体的酶联免疫吸附测定法是一种中度敏感(82%),非侵入性方法,但它是非特异性的(38%)。虽然培养是特异性的(100%),但它比组织学检查敏感(68%)。。
发表日期: 1991-12-04
英文标题: Helicobacter pylori infection: a novel risk factor in the etiology of gastric cancer.
中文标题: 幽门螺杆菌感染:胃癌病因中的新危险因素。
论文作者: Forman D.
英文摘要:
中文摘要:
发表日期: 1991-12-01
中文标题: 幽门螺杆菌相关性胃炎在艾滋病中很少见:抗生素作用还是免疫缺陷的后果?
论文作者: Edwards PD(1), Carrick J, Turner J, Lee A, Mitchell H, Cooper DA.
英文摘要: Helicobacter pylori is the principal cause of type B histologic gastritis. AIDS is associated with increased susceptibility to Gram-negative enteric infections. Using a retrospective study design, we have determined the prevalence of H. pylori-associated histologic gastritis (based on gastric histopathology) in 201 patients with AIDS. These data were compared with H. pylori prevalence rates (based on serology) from healthy HIV-negative, age-matched Australian controls (n = 785) and a cohort (n = 137) of HIV-negative, dyspeptic patients undergoing panendoscopy and antral biopsy, at a community endoscopy center. Twenty-five of 201 (12.5%) patients with AIDS had histologic gastritis and, of these, six (25%) had H. pylori. The 3% (six of 201) H. pylori prevalence rate in the AIDS patients was significantly less than age-matched HIV-negative controls (22%) p = 0.001 and endoscopy center controls (59%) p = 0.009. The explanation for this unexpectedly low prevalence of H. pylori is not possible from these data. It may be a consequence of antimicrobial therapy. Other potential explanations may include specific HIV-related host factors, including hypochlorhydria or an inadequate mucosal inflammatory response, which may impair successful colonization of H. pylori.
中文摘要: 。艾滋病与革兰氏阴性肠道感染的易感性增加有关。使用回顾性研究设计,我们确定了201名艾滋病患者中幽门螺杆菌相关组织学胃炎(基于胃组织病理学)的患病率。这些数据与健康的HIV阴性,年龄匹配的澳大利亚对照组(n=785)和接受全内镜检查和胃窦活检的HIV阴性,消化不良患者队列(n=137)的幽门螺杆菌患病率(基于血清学)进行了比较,在社区内窥镜检查中心。201例艾滋病患者中有25例(12.5%)患有组织学胃炎,其中6例(25%)患有幽门螺杆菌。艾滋病患者中3%(6/201)的幽门螺杆菌患病率显着低于年龄匹配的HIV阴性对照组(22%)p=0.001和内镜中心对照组(59%)p=0.009。从这些数据中不可能解释幽门螺杆菌出人意料的低流行率。这可能是抗菌治疗的结果。其他可能的解释可能包括特定的HIV相关宿主因素,包括胃酸不足或粘膜炎症反应不足,这可能会损害幽门螺杆菌的成功定植。
发表日期: 1991-12-01
英文标题: Restriction fragment length polymorphism of Helicobacter pylori using the urease gene probe.
中文标题: 使用尿素酶基因探针检测幽门螺杆菌的限制性片段长度多态性。
论文作者: Tonokatsu Y(1), Hayashi T, Mizuta T, Yamamoto I, Fukuda Y, Tamura K, Tamura T, Shimoyama T.
英文摘要:
中文摘要:
发表日期: 1991-12-01
英文标题: Detection of Helicobacter pylori in stomach tissue by use of a monoclonal antibody.
中文标题: 使用单克隆抗体检测胃组织中的幽门螺杆菌。
论文作者: Husson MO(1), Leclerc H.
英文摘要: Monoclonal antibodies were produced against an acid glycine extract of Helicobacter pylori ATCC 43504T. One of these appeared to be specific for H. pylori; it recognized all H. pylori isolates by an indirect immunofluorescence assay (IIF) but it did not cross-react with the other strains tested, including different species of the genera Helicobacter, Campylobacter, and Wolinella. Different strains of members of the families Enterobacteriaceae and Pseudomonadaceae or other gram-negative bacteria tested also gave negative reactions. Indirect immunofluorescence assay of antral biopsy specimens identified 54 of 56 infected patients (96.4%), and it may be able to detect nonviable organisms after antibiotic therapy.
中文摘要: 产生针对幽门螺杆菌ATCC 43504T的酸性甘氨酸提取物的单克隆抗体。其中之一似乎对幽门螺杆菌具有特异性;它通过间接免疫荧光测定(IIF)识别所有幽门螺杆菌分离株,但它不与其他测试菌株交叉反应,包括不同种类的螺杆菌属,弯曲杆菌属和Wolinella属。肠杆菌科和假单胞菌科或其他革兰氏阴性细菌家族成员的不同菌株也产生了阴性反应。胃窦活检标本的间接免疫荧光测定确定了56例感染患者中的54例(96.4%),并且它可能能够在抗生素治疗后检测到不存活的生物体。
发表日期: 1991-12-01
中文标题: 胃部分切除术对消化性溃疡患者血清抗幽门螺杆菌免疫球蛋白的影响。
论文作者: Lamers CB(1), Rieu PN, Veenendaal RA, van Duijn W, Offerhaus GJ, Joosten HJ, Peña AS.
英文摘要: Since biliary enterogastric reflux is suggested to eradicate gastric infection with Helicobacter pylori (HP), we have investigated in a prospective randomized study the effect of partial gastrectomy with either Billroth II or Roux-en-Y anastomosis on infection with HP as assessed by the titers of IgG and IgA antibodies against HP in serum. These antibodies were measured by ELISA in serum of 22 patients before and at 10 days and 6, 15, and 24 months after either Billroth II (N = 11) or Roux-en-Y (N = 11) gastrectomy for peptic ulcer. All patients had HP demonstrated in their preoperative endoscopic gastric biopsies. The preoperative serum IgA antibodies against HP (anti-HP IgA) were increased in 20 of the 22 patients (range 0.21-1.69) while the IgG antibodies (anti-HP IgG) were increased in all 22 patients (range 0.38-1.31). Four of the Billroth II patients had clearance of HP from gastric biopsies accompanied by rapid and pronounced decrease of anti-HP IgA. In contrast, the patients with Roux-en-Y gastrectomy and the Billroth II patients with persistent HP infection had no change in anti-HP IgA after surgery. Anti-HP IgG showed variable results in the four patients without gastric HP infection and was not affected by gastrectomy in the patients with persistent HP infection. We concluded that serum anti-HP IgA, but not anti-HP IgG, is helpful in identifying those patients in whom HP is no longer demonstrable after Billroth II gastrectomy. Gastrectomy with Roux-en-Y anastomosis had no effect on gastric HP infection.
中文摘要: 由于胆汁-肠胃反流被认为可以根除幽门螺杆菌(HP)的胃感染,因此我们在一项前瞻性随机研究中研究了部分胃切除术与Billroth II或Roux-en-Y吻合术对HP感染的影响,通过血清中抗HP的IgG和IgA抗体的滴度进行评估。在Billroth II(N=11)或Roux-en-Y(N=11)胃切除术治疗消化性溃疡之前和之后10天以及6,15和24个月,通过ELISA在22名患者的血清中测量这些抗体。所有患者术前内镜胃活检均显示HP。22例患者中有20例(范围0.21-1.69)术前血清抗HP IgA抗体(抗HP IgA)升高,而所有22例患者(范围0.38-1.31)的IgG抗体(抗HP IgG)升高。Billroth II患者中有四名从胃活检中清除了HP,同时抗HP IgA迅速且明显降低。相比之下,Roux-en-Y胃切除术患者和持续HP感染的Billroth II患者术后抗HP IgA没有变化。抗HP IgG在四名无胃HP感染的患者中显示出不同的结果,并且在持续性HP感染的患者中不受胃切除术的影响。我们得出的结论是,血清抗HP IgA而非抗HP IgG有助于识别Billroth II胃切除术后HP不再明显的患者。Roux-en-Y吻合的胃切除术对胃HP感染没有影响。
发表日期: 1991-12-01
英文标题: Helicobacter pylori and the etiology of duodenal ulcer: necessary but not sufficient.
中文标题: 幽门螺杆菌与十二指肠溃疡的病因:必要但不充分。
论文作者: Feldman M.
英文摘要:
中文摘要:
发表日期: 1991-12-01
中文标题: 通过1-D SDS-PAGE蛋白模式的数值分析来区分幽门螺杆菌菌株:治疗后复发的证据。
论文作者: Costas M(1), Morgan DD, Owen RJ, Morgan DR.
英文摘要: Twenty-three pre- and post-treatment isolates of Helicobacter pylori from the antral mucosa of eight patients with dyspepsia and gastritis were compared using 1-D SDS PAGE of proteins. The protein patterns were highly reproducible and were used as the basis for two numerical analyses. The first, based on the total protein patterns, showed that a number of the strains did not cluster with their respective patient set. This was thought to be due to differences in both mobility and intensity of proteins in the major band region. The second analysis, based on partial patterns, excluding the major band region (51-68 kDa), divided the clinical isolates into clearly defined groups corresponding to the patient sets. Although there was a degree of heterogeneity with respect to protein pattern between the pre- and post-treatment isolates of some patients, there was nonetheless clear evidence that each patient was harbouring strains of only a single type. These results suggested that patients were not being reinfected with a different strain but that there was recrudescence of the pre-treatment strain. Protein 'fingerprints' provided a precise and reproducible means of strain differentiation, and revealed that in each patient the same strain persisted after drug therapy even though there was marked patient-to-patient strain variation.
中文摘要: 使用蛋白质的1-D SDS PAGE比较了来自8名消化不良和胃炎患者的胃窦粘膜的23种治疗前和治疗后的幽门螺杆菌分离株。蛋白质模式具有高度可重复性,并被用作两个数值分析的基础。首先,基于总蛋白质模式,表明许多菌株没有与其各自的患者集聚集。这被认为是由于主要条带区域中蛋白质的迁移率和强度的差异。第二次分析基于部分模式,不包括主带区域(51-68 kDa),将临床分离株分为与患者组相对应的明确定义的组。尽管某些患者的治疗前和治疗后分离株之间的蛋白质模式存在一定程度的异质性,但仍有明确证据表明每位患者仅携带单一类型的菌株。。蛋白质“指纹”提供了一种精确且可重复的菌株分化方法,并揭示了在每位患者中,即使存在明显的患者间菌株变异,药物治疗后相同的菌株仍然存在。
发表日期: 1991-12-01
英文标题: Evaluation of liquid media for growth of Helicobacter pylori.
中文标题: 评估幽门螺杆菌生长的液体培养基。
论文作者: Shahamat M(1), Mai UE, Paszko-Kolva C, Yamamoto H, Colwell RR.
英文摘要: Helicobacter pylori has routinely been isolated and grown on solid media. Recently, we have succeeded in obtaining growth of this organism in several liquid media in large volumes, including tryptic soy broth, Mueller-Hinton broth, brucella broth, brain heart infusion broth, and Columbia broth. Growth was tested in the media with and without supplementation. Growth was obtained after incubation under microaerobic conditions and with CO2 enrichment. Growth in a stationary system versus that in an agitated system was evaluated. Results from these experiments show that H. pylori can be grown in any of the liquid media tested except buffered yeast extract-alpha-ketoglutarate if serum is added. No growth was observed on buffered yeast extract-alpha-ketoglutarate even with serum and other supplementation. Growth of H. pylori in most of the liquid media with supplements was improved if the culture was incubated in a CO2 atmosphere. The findings reported here may be useful in clinical, industrial, and research laboratories that require harvests of large quantities of H. pylori cells.
中文摘要: 幽门螺杆菌通常在固体培养基上分离和生长。最近,我们成功地在几种液体培养基中大量获得了这种生物体的生长,包括胰蛋白酶大豆肉汤,米勒-辛顿肉汤,布鲁氏菌肉汤,脑心浸液肉汤和哥伦比亚肉汤。在有和没有补充的培养基中测试生长。在微需氧条件下孵育并富集CO2后获得生长。评估了固定系统与搅拌系统中的生长。这些实验的结果表明,如果添加血清,幽门螺杆菌可以在除缓冲酵母提取物α-酮戊二酸外的任何测试液体培养基中生长。即使使用血清和其他补充剂,缓冲酵母提取物α-酮戊二酸也没有观察到生长。如果将培养物在CO2气氛中孵育,则大多数含有补充剂的液体培养基中幽门螺杆菌的生长得到改善。这里报道的发现可能对需要收获大量幽门螺杆菌细胞的临床,工业和研究实验室有用。
发表日期: 1991-12-01
英文标题: Effect of biliary tract procedures on duodenogastric reflux and the gastric mucosa.
中文标题: 胆道手术对十二指肠胃反流和胃粘膜的影响。
论文作者: Kellosalo J(1), Alavaikko M, Laitinen S.
英文摘要: The effect of alterations in the biliary tract on the gastric milieu was evaluated in gallstone disease and after cholecystectomy or biliary fenestration and compared with a control group. Endoscopic bile reflux was often found in gallstone patients (67%) and almost always after cholecystectomy (89%). Gastric bile acid concentrations were greater in the gallstone patients than in the control patients, were higher still after cholecystectomy, and were highest after biliary fenestration. The pH of the gastric fluid was more alkaline in the cholecystectomized groups. Lysolecithin concentrations were generally low and did not differ between the groups, nor was there any difference in scintigraphic reflux between the groups. Endoscopic erythematous gastritis correlated with the grade of bile reflux and was a common finding after biliary tract procedures. There were no consistent histologic findings associated with duodenogastric reflux. Helicobacter pylori colonization rates were similar in the various patient groups and were not affected by the reflux grade.
中文摘要: 在胆结石病和胆囊切除术或胆道开窗术后评估胆道改变对胃环境的影响,并与对照组进行比较。胆结石患者(67%)经常发现内镜下胆汁反流,几乎总是在胆囊切除术后(89%)。胆囊结石患者的胃胆汁酸浓度高于对照组,胆囊切除术后仍较高,胆道开窗术后最高。胆囊切除组胃液的pH值更碱性。溶血卵磷脂浓度通常较低,组间无差异,组间闪烁反流也无差异。内镜下红斑性胃炎与胆汁反流程度相关,是胆道手术后的常见发现。没有与十二指肠胃反流相关的一致组织学发现。不同患者组的幽门螺杆菌定植率相似,不受反流程度的影响。
发表日期: 1991-12-01
中文标题: 幽门螺杆菌的简化单样本13碳尿素呼气试验:与组织学,培养和ELISA血清学的比较。
论文作者: Logan RP(1), Polson RJ, Misiewicz JJ, Rao G, Karim NQ, Newell D, Johnson P, Wadsworth J, Walker MM, Baron JH.
英文摘要: There is no ideal method for detecting Helicobacter pylori. The 'standard' 13Carbon urea breath test (13C-UBT), which involves collecting eight to 15 breath samples and subsequent costly analysis, was modified by pooling 21 samples of expired breath taken at five minute intervals for 40 minutes into a collecting bag, from which a single 20 ml aliquot was taken and analysed by mass spectrometry. This test was evaluated on 50 patients after routine upper gastrointestinal endoscopy, and results were compared with those from the standard 13C-UBT, bacteriology, ELISA serology, and histology--the latter being taken as the gold standard. H pylori were seen in 34 of 50 (68%) patients (in three it was detected in biopsy specimens from the corpus alone). The modified 13C-UBT was positive (pooled excretion delta 13CO2 greater than 5 per mil) in 31 patients and negative in 19 (three false negative results), specificity was 100% (standard 13C-UBT 94%) and sensitivity 92% (standard 13C-UBT 93%). The modified 13C-UBT had a coefficient of variation within subjects of 3.7%. For the ELISA serology and culture the specificities were both 100%, but the sensitivities were 82% and 68% respectively. The 13C-UBT results correlated with the grade of histological gastritis. The modified 13C-UBT is simpler, cheaper, more reproducible, and provides an easy non-invasive method for the detection of H pylori.
中文摘要: 没有理想的检测幽门螺杆菌的方法。“标准”13碳尿素呼气试验(13C-UBT)涉及收集8至15个呼气样本并随后进行昂贵的分析,通过将21个以5分钟间隔40分钟的呼气样本汇集到一个收集袋中,从中取出一个20毫升的等分试样并通过质谱分析。在常规上消化道内窥镜检查后,对50名患者进行了这项测试,并将结果与标准13C-UBT,细菌学,ELISA血清学和组织学的结果进行了比较,后者被视为金标准。50名患者中有34名(68%)发现了幽门螺杆菌(其中3名仅在语料库的活检标本中检测到)。改良的13C-UBT在31例患者中呈阳性(合并排泄δ13CO2大于5/mil),在19例患者中呈阴性(三个假阴性结果),特异性为100%(标准13C-UBT 94%),敏感性为92%(标准13C-UBT 93%)。改良的13C-UBT在受试者中的变异系数为3.7%。。13C-UBT结果与组织学胃炎的分级相关。改良的13C-UBT更简单,更便宜,更可重复,并且为检测幽门螺杆菌提供了一种简单的非侵入性方法。
发表日期: 1991-12-01
中文标题: 连续一系列接受内窥镜检查的手术患者中幽门弯曲杆菌的发病率。
论文作者: Mohammed R, Kumar S.
英文摘要:
中文摘要:
发表日期: 1991-12-01
英文标题: The effect of bile acids on the growth and adherence of Helicobacter pylori.
中文标题: 胆汁酸对幽门螺杆菌生长和粘附的影响。
论文作者: Mathai E(1), Arora A, Cafferkey M, Keane CT, O'Morain C.
英文摘要: Bile reflux gastritis occurs in the absence of Helicobacter pylori (H. pylori). The aim of this study was to see if the bile acids cheno or ursodeoxycholic acid affected the growth or adherence of H. pylori in vitro. Twenty-seven strains growth were inhibited by 0.1% chenodeoxycholic acid whereas only 11 out of the 27 were inhibited by 0.1% ursodeoxycholic acid. Growth was totally inhibited by a combination of 0.05% chenodeoxycholic acid +0.05% ursodeoxycholic acid. Chenodeoxycholic acid was a more effective inhibitor of adherence in that the number inhibited and percentage inhibition were greater than with ursodeoxycholic acid. Bile salts might be useful in the treatment of H. pylori infection.
中文摘要: 胆汁反流性胃炎发生在没有幽门螺杆菌(幽门螺杆菌)的情况下。这项研究的目的是观察胆汁酸cheno或熊去氧胆酸是否影响体外幽门螺杆菌的生长或粘附。0.1%鹅去氧胆酸抑制了27株菌株的生长,而0.1%熊去氧胆酸抑制了27株菌株中的11株。0.05%鹅去氧胆酸+0.05%熊去氧胆酸的组合完全抑制了生长。鹅去氧胆酸是一种更有效的粘附抑制剂,因为抑制的数量和抑制百分比大于熊去氧胆酸。胆汁盐可能有助于治疗幽门螺杆菌感染。
发表日期: 1991-12-01
英文标题: [The significance of Helicobacter pylori in the pathogenesis of peptic ulcer].
中文标题: [幽门螺杆菌在消化性溃疡发病机制中的意义]。
论文作者: Perederiĭ VG, Subbotin IuV, Tsvetkov AV.
英文摘要:
中文摘要:
发表日期: 1991-12-01
英文标题: Enhanced clearing of Helicobacter pylori after omeprazole plus roxithromycin treatment.
中文标题: 奥美拉唑联合罗红霉素治疗后幽门螺杆菌清除率提高。
论文作者: Cellini L(1), Marzio L, Di Girolamo A, Allocati N, Grossi L, Dainelli B.
英文摘要: The in vitro antibacterial activity of omeprazole against eight strains of Helicobacter pylori was evaluated. Minimum inhibitory concentration (MIC) values were 32 micrograms/ml and 64 micrograms/ml (MIC50 and MIC90 respectively). We performed a randomized single blind study comparing the efficacy of omeprazole alone (for 4 weeks) or combined with roxithromycin (for 2 weeks) in the treatment of duodenal ulcer and chronic active gastritis associated with H. pylori infection, H. pylori was eradicated in 75% of patients treated with omeprazole alone whereas the patients treated with the combination of these drugs were completely free from H. pylori at the end of the therapy.
中文摘要: 评价了奥美拉唑对8株幽门螺杆菌的体外抗菌活性。最小抑制浓度(MIC)值分别为32微克/毫升和64微克/毫升(分别为MIC50和MIC90)。我们进行了一项随机单盲研究,比较单独使用奥美拉唑(持续4周)或联合罗红霉素(持续2周)治疗十二指肠溃疡和与幽门螺杆菌感染相关的慢性活动性胃炎的疗效,幽门螺杆菌根除率为75%单用奥美拉唑治疗的患者,而联合使用这些药物的患者在治疗结束时完全没有幽门螺杆菌。
发表日期: 1991-12-01
中文标题: 幽门螺杆菌不是N-甲基-N'-硝基-N-亚硝基胍诱导的大鼠胃癌发生的共致癌物。
论文作者: Kawaura A(1), Yamamoto I, Tanida N, Inouye H, Takahashi A, Tonokatsu Y, Sawada Y, Sawada K, Shimoyama T.
英文摘要: Helicobacter pylori was implicated in gastric carcinogenesis through the induction of metaplasia of the gastric mucosa. In this experiment a co-carcinogenic effect of H. pylori on chemically induced gastric carcinogenesis was examined. Wistar WKY male rats received drinking water containing 50 mg/l of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and intragastric administration of 10(6) to 10(8) colony forming unit of H. pylori thrice a week for 40 weeks. Thus, 3 groups were assigned as Group I; MNNG alone, Group II; MNNG + vehicle, and Group III; MNNG + H. pylori (n = 30, each). At autopsy, 9 rats (30%) had 7 glandular stomach and 3 duodenal tumors in Group I, and 10 rats (33%) had 8 glandular stomach and 2 duodenal tumors in Group II, whereas in Group III 4 rats (13%) had 2 glandular stomach and 2 duodenal tumors (chi 2 = 4.257, P < 0.15 for the incidences of glandular stomach tumors among 3 groups). The finding seems to suggest that H. pylori has an ambitendency in the gastritis-metaplasia-carcinogenesis sequence as a promoter for the induction of the predisposing mucosa and as an inhibitor against certain carcinogens.
中文摘要: 幽门螺杆菌通过诱导胃粘膜的化生而参与胃癌的发生。在该实验中,检查了幽门螺杆菌对化学诱导的胃癌发生的共致癌作用。Wistar WKY雄性大鼠接受含有50 mg/l N-甲基-N'-硝基-N-亚硝基胍(MNNG)的饮用水,并每周三次灌胃10(6)至10(8)个幽门螺杆菌菌落形成单位,持续40周。因此,将3组分配为第一组;;MNNG+车辆和第三组;MNNG+幽门螺杆菌(每组n=30)。尸检时,I组有9只大鼠(30%)有7个腺胃和3个十二指肠肿瘤,II组有10只大鼠(33%)有8个腺胃和2个十二指肠肿瘤,而III组有4只大鼠(13%)有2个腺胃和2个十二指肠肿瘤(3组中腺胃肿瘤的发生率chi 2=4.257,P<0.15)。这一发现似乎表明,幽门螺杆菌在胃炎化生癌变序列中具有双重倾向,作为诱导易感粘膜的促进剂和针对某些致癌物的抑制剂。
发表日期: 1991-12-01
中文标题: 基底膜蛋白IV型胶原和层粘连蛋白与胃病原体幽门螺杆菌的高亲和力结合。
论文作者: Trust TJ(1), Doig P, Emödy L, Kienle Z, Wadström T, O'Toole P.
英文摘要: The ability of 16 isolates of the human gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled tissue proteins was quantitated by liquid-phase assay. While capable of binding generally low levels of collagen types I and II, vitronectin, and fibronectin (average binding, 8%; highest binding, 23%), the various H. pylori isolates were good binders of the basement membrane proteins collagen type IV and laminin (average binding, 27%; highest binding, 60%). Campylobacter species tested bound lower levels of collagen type IV and laminin (average binding, 12%; highest binding, 17%). Trypsin and proteinase K treatment of H. pylori cells markedly reduced the binding of collagen type IV and laminin, as did heat treatment, suggesting that the binding of basement membrane proteins is mediated by bacterial surface proteins. Binding of both basement membrane proteins was rapid and saturable. 125I-collagen type IV binding to H. pylori 915 was inhibited by preincubation with unlabelled collagen type IV but was not inhibited by laminin or a number of other proteins. Once bound, radiolabelled collagen type IV but was not displaced by an excess of unlabelled collagen type IV, indicating that the binding interaction was of high affinity. Binding of laminin was partially reversible, and analysis in a solid-phase nonradiolabel assay showed that the interaction was of high affinity, with a Kd of 7.9 nM. This interaction was affected by salt, indicating the presence of a hydrophobic component in the ability of H. pylori to bind laminin.
中文摘要: 。虽然能够结合通常低水平的I型和II型胶原蛋白,玻连蛋白和纤连蛋白(平均结合率为8%;最高结合率为23%),但各种幽门螺杆菌分离株是基底膜蛋白IV型胶原蛋白和层粘连蛋白的良好结合物(平均结合率为27%;最高结合率为60%)。测试的弯曲杆菌种类结合了较低水平的IV型胶原和层粘连蛋白(平均结合率为12%;最高结合率为17%)。与热处理一样,幽门螺杆菌细胞的胰蛋白酶和蛋白酶K处理显着降低了IV型胶原和层粘连蛋白的结合,表明基底膜蛋白的结合是由细菌表面蛋白介导的。两种基底膜蛋白的结合都是快速且可饱和的。通过与未标记的IV型胶原预孵育来抑制125I IV型胶原与幽门螺杆菌915的结合,但不受层粘连蛋白或许多其他蛋白质的抑制。一旦结合,放射性标记的IV型胶原蛋白就不会被过量的未标记的IV型胶原蛋白取代,这表明结合相互作用具有高亲和力。层粘连蛋白的结合是部分可逆的,并且在固相非放射性标记测定中的分析表明该相互作用具有高亲和力,Kd为7.9 nM。这种相互作用受到盐的影响,表明幽门螺杆菌结合层粘连蛋白的能力中存在疏水成分。
发表日期: 1991-12-01
英文标题: You can teach old dogma to do new tricks.
中文标题: 你可以教旧教条做新把戏。
论文作者: Brady CE 3rd(1).
英文摘要:
中文摘要:
发表日期: 1991-11-16
英文标题: One week eradication regimen for Helicobacter pylori.
中文标题: 幽门螺杆菌一周根除方案。
论文作者: Logan RP(1), Gummett PA, Misiewicz JJ, Karim QN, Walker MM, Baron JH.
英文摘要: Although Helicobacter pylori is both a frequent cause of gastritis and an important factor in duodenal ulcer recurrence, no treatment regimen exists that is completely safe and effective. We have studied a short eradication regimen of tripotassium dicitrato bismuthate 120 mg four times daily and amoxycillin 500 mg four times daily for seven days with metronidazole 400 mg five times daily for three days (days 5-7). 106 patients with peptic ulceration and non-ulcer dyspepsia, who were also infected with H pylori, were entered into the study. H pylori was successfully eradicated in 76/106 (72%) patients (median follow-up 9.3 months). The rate of eradication was higher among patients with metronidazole-sensitive H pylori (40/43, 93%). In 17/30 patients in whom eradication failed, pretreatment metronidazole-resistant strains were subsequently isolated. Side-effects were mild, the commonest (24/106, 24%) being taste disturbance with metronidazole. A one-week eradication regimen is a safe, effective, cheap, and well-tolerated treatment for metronidazole-sensitive H pylori.
中文摘要: 尽管幽门螺杆菌既是胃炎的常见原因,也是十二指肠溃疡复发的重要因素,但尚无完全安全有效的治疗方案。我们研究了一种短期根除方案,即每天四次120毫克二柠檬酸铋三钾和每天四次500毫克阿莫西林,持续七天,每天五次400毫克甲硝唑,持续三天(第5-7天)。106名同时感染幽门螺杆菌的消化性溃疡和非溃疡性消化不良患者被纳入研究。76/106(72%)患者成功根除幽门螺杆菌(中位随访9.3个月)。。在根除失败的17/30患者中,随后分离出治疗前甲硝唑耐药菌株。副作用轻微,最常见的(24/106,24%)是甲硝唑的味觉障碍。一周的根除方案是一种安全,有效,廉价且耐受性良好的甲硝唑敏感性幽门螺杆菌治疗方法。
发表日期: 1991-11-09
英文标题: Screening for Helicobacter pylori.
中文标题: 筛查幽门螺杆菌。
论文作者: Vaira D, Miglioli M, Holton J, Mulè P, Barbara L.
英文摘要:
中文摘要:
发表日期: 1991-11-01
英文标题: [Helicobacter (Campylobacter) pylori in stomach and duodenal biopsies].
中文标题: [胃和十二指肠活检中的幽门螺杆菌(弯曲杆菌)。
论文作者: Chlumská A(1), Chlumský J Jr, Skála I, Marecková O, Pechancová J.
英文摘要: In bioptic specimens of the gastric mucosa of 57 patients with dyspeptic complaints and/or a duodenal ulcer Helicobacter pylori was detected under the microscope in 82,4% of chronic active gastritis and in 28,6% and 61,5% resp. of chronic gastritis grade I and II. The finding of helicobacteria depended on the number of collected specimens. They were never found at sites with intestinal metaplasia and their numbers did not correspond to the intensity and character of the inflammatory changes. As compared with the antrum, in the corpus of the stomach helicobacteria were found more frequently not only in mild forms of the inflammation but also in the normal mucosa. In the duodenum the finding was negative and the inflammatory changes were only mild. In all patients with a duodenal ulcer and a scar after ulceration in the antrum chronic active or inactive gastritis grade II was found with helicobacteria in 84,6% of the observations. Cultivation was consistent with microscopic evidence in 50 patients (87,7%).
中文摘要: 在57例消化不良和/或十二指肠溃疡患者的胃粘膜活检标本中,在显微镜下检测到82.4%的慢性活动性胃炎和28.6%和61.5%的幽门螺杆菌。慢性胃炎I级和II级。螺杆菌的发现取决于采集标本的数量。。与胃窦相比,在胃体中,不仅在轻度炎症中,而且在正常粘膜中,螺杆菌更常见。在十二指肠中,发现为阴性,炎症变化仅为轻度。在所有患有十二指肠溃疡和胃窦溃疡后瘢痕的患者中,84.6%的观察结果发现慢性活动性或非活动性胃炎II级为螺杆菌。50名患者(87.7%)的培养与显微镜证据一致。
发表日期: 1991-11-01
英文标题: Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.
中文标题: 直接聚合酶链反应检测人类和动物中的幽门螺杆菌。
论文作者: Ho SA(1), Hoyle JA, Lewis FA, Secker AD, Cross D, Mapstone NP, Dixon MF, Wyatt JI, Tompkins DS, Taylor GR, et al.
英文摘要: We designed a polymerase chain reaction (PCR) for amplifying the Helicobacter pylori gene encoding 16S rRNA. Primers for the specific detection of H. pylori were designed for areas of the 16S rRNA gene in which there is the least sequence homology between H. pylori and its closest relatives. The specificity of detection was confirmed by ensuring that the primers did not amplify DNA extracts from the campylobacters H. cinaedi, H. mustelae, and Wolinella succinogenes, which are the closest relatives of H. pylori, as determined by 16S rRNA sequencing. Serial dilution experiments revealed the detection of as little as 0.1 pg of DNA by PCR and 0.01 pg by nested PCR. H. pylori DNA was detected successfully in clinical paraffin-embedded and fresh gastric biopsy specimens from patients positive for the bacterium and also in fecal suspensions seeded with the organism. The DNA from the nonculturable coccoid form of H. pylori was also identified by the primers. Universal primers designed for highly conserved areas on the 16S rRNA gene enabled large amplification products to be produced for direct sequencing analysis. Gastric bacteria resembling H. pylori have been isolated from animals. DNA of these animal gastric bacteria amplified with H. pylori-specific primers yielded PCR products identical to those from human isolates of H. pylori, as confirmed by the use of a 20-base radiolabelled probe complementary to an internal sequence flanked by the H. pylori-specific primers. The results of PCR amplification and partial 16S rRNA gene sequence analysis strongly support the contention that the gastric organisms previously recovered from a pig, a baboon, and rhesus monkeys are H. pylori.
中文摘要: 我们设计了一种聚合酶链反应(PCR)来扩增编码16S rRNA的幽门螺杆菌基因。针对幽门螺杆菌与其近亲之间序列同源性最低的16S rRNA基因区域设计了特异性检测幽门螺杆菌的引物。通过16S rRNA测序确定,通过确保引物不扩增来自幽门螺杆菌最接近亲属的弯曲杆菌H.cinaedi,H.mustelae和Wolinella succinogenes的DNA提取物,证实了检测的特异性。连续稀释实验显示,通过PCR检测到的DNA少至0.1 pg,通过巢式PCR检测到的DNA少至0.01 pg。H、 在临床石蜡包埋和新鲜胃活检标本中成功检测到幽门螺杆菌DNA,这些标本来自细菌阳性患者以及接种该生物体的粪便悬浮液。引物还鉴定了来自不可培养的球形幽门螺杆菌的DNA。为16S rRNA基因高度保守区域设计的通用引物能够产生用于直接测序分析的大型扩增产物。已经从动物中分离出类似幽门螺杆菌的胃细菌。通过使用与幽门螺杆菌特异性引物侧翼的内部序列互补的20碱基放射性标记探针证实,用幽门螺杆菌特异性引物扩增的这些动物胃细菌的DNA产生的PCR产物与来自幽门螺杆菌人分离株的PCR产物相同。PCR扩增和部分16S rRNA基因序列分析的结果强烈支持以下论点:先前从猪,狒狒和恒河猴中回收的胃生物是幽门螺杆菌。
发表日期: 1991-11-01
英文标题: Long term serological surveillance after treatment of Helicobacter pylori infection.
中文标题: 幽门螺杆菌感染治疗后的长期血清学监测。
论文作者: Veenendaal RA(1), Peña AS, Meijer JL, Endtz HP, van der Est MM, van Duijn W, Eulderink F, Kreuning J, Lamers CB.
英文摘要: Fifteen patients with type B gastritis caused by Helicobacter pylori infection were treated with 'triple' therapy consisting of colloidal bismuth subcitrate, amoxycillin, and metronidazole. All were followed up as outpatients every three months for at least one year. After 'triple' therapy a significant (p less than 0.01) and persistent reduction in IgA and IgG antibody levels against H pylori was detected. In three patients recurrent active infection with H pylori at nine and 12 months was detected by a rise in IgA (three patients) and IgG (two patients) antibody levels against H pylori and worsening of symptoms, and was confirmed by culture and histology. In 11 patients, the absence of infection at 12 months was confirmed by culture and histology. In a control group of 13 patients with type B gastritis who received no antibacterial treatment, specific IgA and IgG antibody levels against H pylori remained unchanged during 12 months of follow up. Although specific IgG against H pylori is the most widely used serological test for screening, our data indicate that specific IgA is also valuable in monitoring treatment. These serological tests are easy to perform, relatively inexpensive, devoid of radioactivity and are very acceptable to patients. It is concluded that serological testing is the preferred method for follow up after treatment for H pylori infection and will probably replace endoscopy or the urea breath test.
中文摘要: 对15例由幽门螺杆菌感染引起的B型胃炎患者进行了由胶体次柠檬酸铋,阿莫西林和甲硝唑组成的“三联”治疗。每三个月对所有患者进行一次门诊随访,至少一年。在“三联”治疗后,检测到针对幽门螺杆菌的IgA和IgG抗体水平显着降低(p小于0.01)并持续降低。在三名患者中,通过抗幽门螺杆菌的IgA(三名患者)和IgG(两名患者)抗体水平升高和症状恶化,检测到9个月和12个月时幽门螺杆菌复发性活动性感染,并通过培养和组织学证实。在11例患者中,培养和组织学证实12个月时没有感染。在13名未接受抗菌治疗的B型胃炎患者的对照组中,在12个月的随访期间,针对幽门螺杆菌的特异性IgA和IgG抗体水平保持不变。尽管针对幽门螺杆菌的特异性IgG是筛查中使用最广泛的血清学检测方法,但我们的数据表明,特异性IgA在监测治疗中也很有价值。这些血清学检测容易进行,相对便宜,没有放射性,患者非常接受。结论是,血清学检测是幽门螺杆菌感染治疗后随访的首选方法,可能会取代内窥镜检查或尿素呼气试验。
发表日期: 1991-11-01
英文标题: Helicobacter pylori infection in healthy people.
中文标题: 健康人群幽门螺杆菌感染。
论文作者: Meyer-Wyss B, Beglinger C, Baselgia L, Merki H, Renner E.
英文摘要:
中文摘要:
发表日期: 1991-11-01
英文标题: Adhesion of Helicobacter pylori to human gastric mucosal biopsy specimens cultivated in vitro.
中文标题: 幽门螺杆菌与体外培养的人胃粘膜活检标本的粘附。
论文作者: Rosberg K(1), Berglindh T, Gustavsson S, Hübinette R, Rolfsen W.
英文摘要: The therapeutic advances in Helicobacter pylori infection is hampered by the lack of suitable animal model systems. We have previously reported on successful adherence of H. pylori to pig gastric mucosa cultured in vitro. The aim of this study was to verify the technique in human biopsy specimens cultured in vitro. Mucosal samples were taken from H. pylori-negative and H. pylori-positive patients undergoing gastric surgery. The non-infected tissue was infected with H. pylori in vitro, and the infected tissue was put into culture immediately. Total number and those H. pylori firmly attached were checked throughout a 72-h culture. Viability of cultured human gastric mucosa was good and unaffected by the presence of H. pylori. The amount of bacteria adhering, increased with time from 0.01% to 2-4% after 72 h in culture. In vivo-infected specimens initially had a low number of firmly attached H. pylori, but total H. pylori increased with time in culture. It is concluded that human gastric biopsy specimens show good viability for 72 h and that viability and cell division of H. pylori were maintained in both in vivo and in vitro H. pylori-infected tissue. In both cases the total number of viable bacteria attached to the specimens increased with incubation time.
中文摘要: 由于缺乏合适的动物模型系统,幽门螺杆菌感染的治疗进展受到阻碍。我们之前曾报道幽门螺杆菌成功粘附于体外培养的猪胃粘膜。这项研究的目的是在体外培养的人体活检标本中验证该技术。粘膜样本取自接受胃手术的幽门螺杆菌阴性和幽门螺杆菌阳性患者。未感染的组织在体外感染幽门螺杆菌,并立即将感染的组织投入培养。在整个72小时培养过程中检查总数和牢固附着的幽门螺杆菌。培养的人胃粘膜活力良好,不受幽门螺杆菌的影响。培养72小时后,细菌粘附量随时间从0.01%增加到2-4%。体内感染的标本最初具有少量牢固附着的幽门螺杆菌,但总幽门螺杆菌随着培养时间的推移而增加。结论是,人胃活检标本在72小时内显示出良好的活力,并且幽门螺杆菌的活力和细胞分裂在体内和体外幽门螺杆菌感染的组织中均得以维持。在这两种情况下,附着在标本上的活细菌总数随着孵育时间的增加而增加。
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