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发表日期： 1992 Sep
中文标题： 定量评估根除幽门螺杆菌后慢性胃炎的组织学变化。
论文作者： Di Napoli, A; Petrino, R; Boero, M; Bellis, D; Chiandussi, L
英文摘要： AIMS: To evaluate the effect of 10 day triple treatment on H pylori eradication and associated gastritis. METHODS: Fifty patients with H pylori positive non-ulcer dyspepsia were treated for 10 days with amoxicillin, tinidazole, and bismuth salts. Histological examination of the antral mucosa was performed before (T0), six weeks (T1), and six months (T2) after treatment. The new Sydney classification of gastritis was used, using a score from 0 to 3 to grade degree of inflammation, atrophy, activity (intraepithelial or lamina propria damage) and H pylori. RESULTS: At T0 all patients had chronic active gastritis. Lymphoid follicules were present in 12 cases. At T1 33 patients were H pylori negative: the score showed a decrease of activity (from 2.5 to 0.54). The result was confirmed at T2 (mean score 0.22). Inflammation decreased from 1.8 to 1.4 at T2. Only one case of follicular gastritis was observed. In H pylori positive patients the scores did not show significant modifications. CONCLUSIONS: Ten day triple treatment is effective in eradicating H pylori in 69% of cases, causing a decrease of the total score for gastritis. Activity, defined by polymorph infiltration, was promptly reduced when H pylori was eradicated. There was a trend to a reduction in inflammation, but atrophy was irreversible.
中文摘要： 目的: 评估10天三联治疗对幽门螺杆菌根除和相关胃炎的影响。方法: 对50例幽门螺杆菌阳性非溃疡性消化不良患者用阿莫西林、替硝唑、铋盐治疗10天。在治疗前 (T0)，六周 (T1) 和六个月 (T2) 后对胃窦粘膜进行组织学检查。使用新的Sydney胃炎分类，使用0到3的评分对炎症，萎缩，活动 (上皮内或固有层损伤) 和幽门螺杆菌进行分级。结果: 在T0时，所有患者均患有慢性活动性胃炎。12例有淋巴滤泡。在T1时，33名患者为幽门螺杆菌阴性: 评分显示活性降低 (从2.5到0.54)。在T2确认结果 (平均得分0.22)。炎症在t2时从1.8减少到1.4。仅观察到1例滤泡性胃炎。在幽门螺杆菌阳性患者中，得分没有显示出明显的变化。结论: 10天三联疗法可有效根除69% 例幽门螺杆菌，从而降低胃炎的总分。当根除幽门螺杆菌时，由多晶型浸润定义的活性迅速降低。炎症有减轻的趋势，但萎缩是不可逆的。
发表日期： 1992 Sep
中文标题： 逆转录和聚合酶链反应扩增rRNA检测螺杆菌。
论文作者： Engstrand, L; Nguyen, A M; Graham, D Y; el-Zaatari, F A
英文摘要： Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. Reverse transcription of rRNA was followed by PCR amplification (RT-PCR) of the synthesized cDNA and 16S rRNA gene. The amplified PCR products were analyzed by agarose gel electrophoresis and Southern blotting. Using ethidium bromide-stained agarose gels, we were able to detect the expected 500-bp DNA fragment from as few as two H. pylori organisms per reaction. The specificity of the RT-PCR assay was tested with 27 clinical isolates and related reference strains; although the number of bacterial cells used per reaction was 10(5)-fold greater than the number of H. pylori organisms used, amplification was detected only with bacteria in the same genus, H. cinaedi and H. mustelae. Ten H. pylori organisms per biopsy specimen were detected on agarose gels when organisms were added to samples prepared from a processed colon biopsy sample. RT-PCR results were consistent with urea breath test and culture results in 14 of 15 gastric biopsy specimens; the specificity was 100%. RT-PCR of rRNA from H. pylori increased the sensitivity of pathogen detection at least 25- to 50-fold compared with that of previous PCR assays. This low level of detection by RT-PCR assay may prove to be well suited for verifying eradication following therapy.Images:
中文摘要： 幽门螺杆菌16srrna的序列数据用于选择两个22个碱基的寡核苷酸引物，用于聚合酶链反应 (PCR) 以检测幽门螺杆菌。用裂解缓冲液处理幽门螺杆菌细胞，煮沸，用氯仿提取。rRNA的逆转录之后是合成的cDNA和16srrna基因的PCR扩增 (rt-pcr)。通过琼脂糖凝胶电泳和Southern印迹分析扩增的PCR产物。使用溴化乙锭染色的琼脂糖凝胶，我们能够从每个反应少至两个幽门螺杆菌生物中检测到预期的500 bp DNA片段。用27种临床分离株和相关参考菌株测试了rt-pcr测定的特异性; 尽管每个反应使用的细菌细胞数量比H数多10(5) 倍。使用的幽门螺杆菌生物，仅在同一属H的细菌中检测到扩增。cinaedi和H.小胡子.当将生物体添加到从经处理的结肠活检样品制备的样品中时，在琼脂糖凝胶上检测到每个活检样品的10个幽门螺杆菌生物体。Rt-pcr结果与15例胃活检标本中14例的尿素呼气试验和培养结果一致，特异性为100%。与以前的PCR分析相比，来自幽门螺杆菌的rRNA的rt-pcr将病原体检测的灵敏度提高了至少25至50倍。这种低水平的rt-pcr检测可能被证明非常适合验证治疗后的根除。
发表日期： 1992 Sep
英文标题： Serological detection of Helicobacter pylori by a flow microsphere immunofluorescence assay.[全文]
中文标题： 通过流动微球免疫荧光法对幽门螺杆菌进行血清学检测。
论文作者： Best, L M; Veldhuyzen van Zanten, S J; Bezanson, G S; Haldane, D J; Malatjalian, D A
英文摘要： A flow cytometric immunofluorescence assay (FMIA) for the detection of immunoglobulin G antibodies to Helicobacter pylori was developed. A multicomponent antigen was prepared and used to coat carboxylated polystyrene microspheres for reaction with patient sera followed by fluorescein isothiocyanate-labelled goat anti-human immunoglobulin G. The reacted microspheres were collected with a flow cytometer, and fluorescence was quantitated relative to the cutoff value provided by pooled sera from patients in whom H. pylori could not be demonstrated by culture or histology. Serum samples from 28 H. pylori-positive patients and 27 H. pylori-negative patients were tested by FMIA. Additionally, an in-house enzyme-linked immunosorbent assay (ELISA) employing the same antigen preparation and a commercially available ELISA were used to assay the patient population. Both the FMIA and in-house ELISA were 100% sensitive and 89% specific with positive and negative predictive values of 90 and 100% and no equivocal results. The commercial ELISA was 96% sensitive and 89% specific with positive and negative predictive values of 90 and 96% and five equivocal results. FMIA provides a rapid, inexpensive, and easily performed means for serodiagnosis of H. pylori.Images:
中文摘要： 开发了一种用于检测幽门螺杆菌免疫球蛋白G抗体的流式细胞免疫荧光测定法 (FMIA)。制备了多组分抗原，并用于包被羧化聚苯乙烯微球，以与患者血清反应，然后与异硫氰酸荧光素标记的山羊抗人免疫球蛋白G反应。用流式细胞仪收集反应的微球，并相对于来自患者的合并血清提供的截止值定量荧光，在所述患者中幽门螺杆菌不能通过培养或组织学证实。通过FMIA测试了来自28位幽门螺杆菌阳性患者和27位幽门螺杆菌阴性患者的血清样品。另外，使用采用相同抗原制剂的内部酶联免疫吸附测定 (ELISA) 和市售ELISA来测定患者群体。FMIA和内部ELISA均具有100% 敏感性和89% 特异性，阳性和阴性预测值分别为90和100%，并且没有模棱两可的结果。商业ELISA具有96% 敏感性和89% 特异性，阳性和阴性预测值分别为90和96%，并且有五个模棱两可的结果。FMIA为幽门螺杆菌的血清诊断提供了一种快速，廉价且易于执行的方法。
发表日期： 1992 Sep
英文标题： Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori.[全文]
中文标题： 血清中和抗体对幽门螺杆菌空泡毒素的反应。
论文作者： Cover, T L; Cao, P; Murthy, U K; Sipple, M S; Blaser, M J
英文摘要： Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme-linked immunosorbent assay (P less than 0.0001). The presence of cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.Images:
中文摘要： 大约50% 的幽门螺杆菌分离株在体外产生诱导真核细胞空泡化的细胞毒素。为了确定这种现象的体内相关性，我们试图检测幽门螺杆菌感染者血清中的细胞毒素中和抗体。作为一组，来自29名幽门螺杆菌感染患者的血清中和纯化的细胞毒素的活性的程度明显高于来自24名未感染者的血清 (P = 0.007)。幽门螺杆菌感染者血清中的细胞毒素中和活性主要由纯化的IgG级分介导。幽门螺杆菌感染者的血清中和了多种幽门螺杆菌菌株产生的细胞毒素，但未能中和三甲胺诱导的细胞空泡作用。人或免疫兔血清对细胞毒素活性的中和与87 kD幽门螺杆菌蛋白的免疫印迹IgG识别有关。类似地，在酶联免疫吸附测定中，血清对毒素的中和与纯化的细胞毒素的IgG识别有关 (P小于0.0001)。幽门螺杆菌感染者血清中细胞毒素中和抗体的存在表明细胞毒素是在体内合成的。
发表日期： 1992 Sep
英文标题： Characteristics of Helicobacter pylori variants selected for urease deficiency.[全文]
中文标题： 选择尿素酶缺乏症的幽门螺杆菌变异的特征。
论文作者： Pérez-Pérez, G I; Olivares, A Z; Cover, T L; Blaser, M J
英文摘要： The urease of Helicobacter pylori is suspected to play a role in the pathogenesis of gastritis. Although all clinical isolates of H. pylori are urease positive (U+), we have selected and characterized several spontaneously arising urease-negative (U-) variants from wild-type strain 60190. Urease-negative variants were identified by growth in medium containing 60 mM urea and arose at a frequency of 10(-5) to 10(-6). The urease activity of the wild-type strain inhibited growth of this strain in the presence of 60 mM urea. U- variants retained the U- phenotype for more than 100 passages on medium with or without urea. The urease activities of the original U+ and derived U- cells were 9.55 to 16.7 and 0.01 to 0.17 U/mg of protein, respectively. Colonial growth and other biochemical characteristics were identical for the strains. U- variants showed three classes of whole-cell sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles: (i) identical to U+; (ii) change in the migration of the 61-kDa urease subunit; and (iii) lack of 61- and 30-kDa subunits. These differences were confirmed by immunoblotting and by protein separation using fast protein liquid chromatography. The U+ strain but not U- variants tolerated exposure to pH 4.0 for 60 min in the presence of urea. Supernatants of the U+ strain and U- variants contained vacuolating cytotoxin activity for HeLa cells in similar titers. By enzyme-linked immunosorbent assay, human serum samples recognized water extract from the U+ strain significantly better than extract from a U- variant lacking urease subunits. In conclusion, this study demonstrates that U- H. pylori variants may arise spontaneously, that urease activity enhances survival at acid pH, and that urease and cytotoxin activities are disparate phenotypes.Images:
中文摘要： 怀疑幽门螺杆菌的脲酶在胃炎的发病机理中起作用。尽管所有幽门螺杆菌的临床分离株均为尿素酶阳性 (U)，但我们已选择并表征了几种来自野生型菌株60190的自发产生的尿素酶阴性 (U-) 变体。通过在含有60 mm尿素的培养基中生长来鉴定尿素酶阴性变体，并且以10(-5) 至10(-6) 的频率出现。在存在60 mM尿素的情况下，野生型菌株的脲酶活性抑制了该菌株的生长。U-变体在有或没有尿素的培养基上保留U-表型超过100代。原始U细胞和衍生的U细胞的脲酶活性分别9.55至16.7和0.01至0.17 U/mg蛋白质。菌株的菌落生长和其他生化特性相同。U变体显示了三类全细胞十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱 :( i) 与U相同; (ii) 61-kda脲酶亚基迁移的变化; (iii) 缺乏61和30-kda亚基。这些差异通过免疫印迹和使用快速蛋白质液相色谱的蛋白质分离来证实。在尿素存在下，U菌株而不是U变体耐受暴露于pH 4.0 60分钟。U菌株和U变体的上清液以相似的滴度含有对HeLa细胞的空泡细胞毒素活性。通过酶联免疫吸附测定，人血清样品识别U菌株的水提取物明显优于缺乏脲酶亚基的U变体的水提取物。总之，这项研究表明，U-幽门螺杆菌变异可能自发产生，脲酶活性增强在酸性pH下的存活，并且脲酶和细胞毒素活性是完全不同的表型。
发表日期： 1992 Sep
英文标题： Mucosal peptic activity during Helicobacter pylori infection in pediatric patients.[全文]
中文标题： 小儿幽门螺杆菌感染期间的粘膜消化活性。
论文作者： Yahav, J; Oderda, G; Diver-Haber, A; Keller, N; Jonas, A
英文摘要： Intramucosal peptic activity may participate in the genesis of acute and chronic superficial gastritis. The proteolytic activity of homogenates of gastric mucosa (antrum and body) and duodenum were measured at pH 2.0 (total peptic activity) after exposure to pH 8.0 (pepsinogen) and the activated pepsinogen (pepsin) was calculated in pediatric patients investigated for the presence of Helicobacter pylori (H pylori), 122 antral, 77 stomach body, and 74 duodenal biopsies were examined in 43 H pylori positive patients, 51 controls, and 28 H pylori negative gastritis patients. Activated pepsinogen was significantly reduced in the stomach of H pylori positive patients only. Pepsinogen values were similar in all the anatomical areas tested in all patients. In 13 H pylori positive patients reinvestigated three months after antibiotic therapy, antral mucosal activated pepsinogen activity increased significantly (mean pretreatment 1.56 (1.0) U/mg protein versus mean post-treatment 2.72 (1.7) U/mg protein) and reached values comparable with controls. The decreased activated pepsinogen activity in association with normal pepsinogen content observed in the antrum of H pylori positive gastritis patients indicate local pepsin inactivation or alternately enhanced removal into the gastric lumen or backflow into the circulation.
中文摘要： 粘膜内消化活性可能参与急性和慢性浅表性胃炎的发生。暴露于8.0 (胃蛋白酶原) 后，在pH 2.0 (总消化活性) 下测量胃粘膜 (胃窦和身体) 和十二指肠匀浆的蛋白水解活性，并计算儿科患者中存在的活化胃蛋白酶原 (胃蛋白酶) 幽门螺杆菌 (H pylori)，122胃窦，在43例H pylori阳性患者，51例对照和28例H pylori阴性胃炎患者中检查了77例胃体和74例十二指肠活检。仅在幽门螺杆菌阳性患者的胃中，激活的胃蛋白酶原显着降低。在所有患者测试的所有解剖区域中，胃蛋白酶原值均相似。在抗生素治疗三个月后重新调查的13例幽门螺杆菌阳性患者中，胃窦粘膜激活的胃蛋白酶原活性显着增加 (治疗前平均1.56 (1.0) U/mg蛋白与治疗后平均2.72 (1.7) U/mg蛋白)，并达到与对照组相当的值。在幽门螺杆菌阳性胃炎患者的胃窦中观察到的活化胃蛋白酶原活性降低与正常胃蛋白酶原含量相关，表明局部胃蛋白酶失活或交替增加进入胃腔或回流到循环中。
发表日期： 1992 Sep
中文标题： 甲硝唑可减轻非甾体抗炎药引起的肠病的肠道炎症和失血。
论文作者： Bjarnason, I; Hayllar, J; Smethurst, P; Price, A; Gumpel, M J
英文摘要： This study assessed the effect of metronidazole on the gastroduodenal mucosa, intestinal permeability, blood loss, and inflammation in patients on non-steroidal anti-inflammatory drugs (NSAIDs). Thirteen patients were studied before and after 2-12 weeks' treatment with metronidazole 800 mg/day, while maintaining an unchanged NSAID intake. Intestinal inflammation, as assessed by the faecal excretion of indium-111 labelled neutrophils, and blood loss, assessed with chromium-51 labelled red cells, were significantly reduced after treatment (mean (SD) 111In excretion 4.7 (4.7)% v 1.5 (1.3)% (N < 1.0%), p < 0.001, 51Cr red cells loss 2.6 (1.6) ml/day v 0.9 (0.5) ml/day (N < 1.0 ml/day), p < 0.01). Intestinal permeability assessed as the 5 hour urinary excretion ratio of 51CrEDTA/L-rhamnose did not change significantly (0.133 (0.046) v 0.154 (0.064), p > 0.1) and there were no significant changes in the endoscopic or microscopic appearances of the gastroduodenal mucosa. These results suggest that the neutrophil is the main damaging effector cell in NSAID induced enteropathy. The main neutrophil chemo-attractant in this enteropathy may be a metronidazole sensitive microbe.
中文摘要： 这项研究评估了甲硝唑对非甾体类抗炎药 (NSAIDs) 患者的胃十二指肠粘膜，肠通透性，失血和炎症的影响。在使用甲硝唑800 mg/天治疗2-12周之前和之后，对13例患者进行了研究，同时保持NSAID摄入量不变。治疗后，通过铟-111标记的中性粒细胞的粪便排泄评估的肠道炎症和用铬-51标记的红细胞评估的失血量显着减少 (平均 (SD) 111in排泄4.7 (4.7)% v 1.5 (1.3)% (N <1.0%)，p <0.001，51Cr红细胞损失2.6(1.6) 毫升/天v 0.9 (0.5) 毫升/天 (N <1.0毫升/天)，p <0.01)。以51credta/L-鼠李糖的5小时尿排泄率评估的肠通透性没有显着变化 (0.133 (0.046) v 0.154 (0.064)，p> 0.1)，并且内镜或显微镜下表现没有显着变化胃十二指肠粘膜。这些结果表明，中性粒细胞是NSAID诱导的肠病的主要损伤效应细胞。在这种肠病中，主要的中性粒细胞化学引诱剂可能是甲硝唑敏感的微生物。
发表日期： 1992 Sep
英文标题： Helicobacter pylori and peptic ulcer recurrence.[全文]
中文标题： 幽门螺杆菌与消化性溃疡复发。
论文作者： Guslandi, M
英文摘要：
中文摘要：
发表日期： 1992 Sep
英文标题： Helicobacter pylori and peptic ulcer recurrence.[全文]
中文标题： 幽门螺杆菌与消化性溃疡复发。
论文作者： Tovey, F I; Yiu, Y C; Husband, E M; Baker, L; Jayaraj, A P
英文摘要：
中文摘要：
发表日期： 1992 Sep
中文标题： 接受肌内金治疗的类风湿关节炎患者抗幽门螺杆菌抗体水平降低。
论文作者： Janssen, M; Dijkmans, B A; Vandenbroucke, J P; van Duijn, W; Peña, A S; Lamers, C B
英文摘要： BACKGROUND: Sodium aurothiomalate has been reported to have in vitro activity against Helicobacter pylori. Intramuscular gold, as given to patients with rheumatoid arthritis (RA), may therefore influence the colonisation of the gastric mucosa with H pylori. METHODS: Two groups were compared. One group of 42 patients was treated with intramuscular gold; the other group of 58 patients was treated with antimalarial drugs. Antibodies to H pylori (IgA and IgG) were assessed by an enzyme linked immunosorbent assay (ELISA) and total IgA and IgG were measured by nephelometry. RESULTS: IgA and IgG antibody titres against H pylori and total IgA and IgG levels were lower in the patients treated with gold than in the group treated with antimalarial drugs. The ratio of IgA antibodies to H pylori to total IgA antibodies and the ratio of IgG antibodies to H pylori to total IgG antibodies were lower in the group treated with gold. The percentage of seropositivity to H pylori was significantly lower in the group treated with gold than in the group treated with antimalarial drugs for the two IgA antibodies (35 and 55% respectively) and IgG antibodies to H pylori (40 and 65% respectively). CONCLUSIONS: Although this study cannot completely exclude the possibility that a suppressive effect of intramuscular gold on total immunoglobulin production plays a part in the decrease in the titres of IgA antibodies to H pylori and IgG antibodies to H pylori, the lower ratios of antibodies to H pylori to total immunoglobulin antibodies and the lower percentages of seropositivity to H pylori in the group treated with gold suggests that treatment with intramuscular gold decreases H pylori colonisation.
中文摘要： 背景: 据报道，硫代苹果酸钠对幽门螺杆菌具有体外活性。因此，给予类风湿性关节炎 (RA) 患者的肌内金可能会影响幽门螺杆菌在胃粘膜中的定植。方法: 对两组进行比较。一组42例患者接受肌内金治疗; 另一组58例患者接受抗疟药物治疗。通过酶联免疫吸附测定 (ELISA) 评估针对幽门螺杆菌的抗体 (IgA和IgG)，并通过比浊法测量总IgA和IgG。结果: 用金治疗的患者抗幽门螺杆菌的IgA和IgG抗体滴度以及总IgA和IgG水平低于用抗疟药物治疗的患者。在用金处理的组中，针对幽门螺杆菌的IgA抗体与总IgA抗体的比率以及针对幽门螺杆菌的IgG抗体与总IgG抗体的比率较低。对于两种针对幽门螺杆菌的IgA抗体 (分别为35和55%) 和IgG抗体 (分别为40和65%)，金治疗组的幽门螺杆菌血清阳性百分比显着低于抗疟药治疗组。结论: 虽然这项研究不能完全排除这种可能性，即肌内金对总免疫球蛋白产生的抑制作用在幽门螺杆菌的IgA抗体和幽门螺杆菌的IgG抗体滴度下降中起作用，在用金治疗的组中，幽门螺杆菌抗体与总免疫球蛋白抗体的较低比率和幽门螺杆菌血清阳性的较低百分比表明，用肌内金治疗可减少幽门螺杆菌定植。
发表日期： 1992 Sep
英文标题： Helicobacter pylori. Its role in the pathogenesis of peptic ulcer disease in a new animal model.[全文]
中文标题： 幽门螺杆菌。在新的动物模型中，它在消化性溃疡病发病机理中的作用。
论文作者： Ross, J. S.; Bui, H. X.; del Rosario, A.; Sonbati, H.; George, M.; Lee, C. Y.
英文摘要： The association and causative role of Helicobacter pylori infection of the stomach with gastric ulcer, duodenal ulcer, non-ulcer dyspepsia, and gastritis has remained controversial. The authors studied the effects of daily intragastric administration of H. pylori suspension in saline (10(8) CFU/ml) and bacteria-free filtrates of saline H. pylori suspensions in 85 Sprague-Dawley rats (weight, 150 to 200 g) with normal mucosa and with surgically produced experimental gastric ulcers. Group I rats (n = 30) with pre-existent experimental gastric ulcers received H. pylori suspension (ATCC 43504, 10(8) CFU/ml); Group II rats (n = 20) with experimental gastric ulcers received bacteria-free H. pylori filtrates; Group III rats with ulcers (n = 20) received saline alone; and Group IV control rats (n = 15) without ulcers received intact H. pylori organisms in suspension (ATCC 43504, 10(8) CFU/ml). At death, ulcer surface areas were measured with a dissecting microscope. Full-thickness sections were obtained for quantitative and qualitative histologic parameters, including the area of remaining mucosal necrosis; characteristics and cellular composition of restored mucosal architectures; and presence or absence of inflammation including counts of neutrophils and lymphocytes. H. pylori organisms were identified within the surface mucus and crypts using routine, special, and immunohistochemical stains. Our results indicate that the continued presence of either intact H. pylori organisms or bacteria-free H. pylori filtrates in the stomachs of rats with pre-existent gastric ulcers resulted in delayed healing of the ulcers and persistence of chronic active inflammation. Daily administration of suspensions of H. pylori organisms to sham-operated rats with intact gastric mucosa, however, resulted in no ulceration or inflammation despite identification of surface H. pylori organisms at death. The authors conclude that H. pylori alone causes little or no effect on an intact gastric mucosa in the rat, that either intact organisms or bacteria-free filtrates cause similar prolongation and delayed healing of pre-existing ulcers with active chronic inflammation, and that the presence of predisposing factors leading to disruption of gastric mucosal integrity may be required for the H. pylori enhancement of inflammation and tissue damage in the stomach.Images: Figure 1Figure 2Figure 3Figure 4Figure 5
中文摘要： 胃幽门螺杆菌感染与胃溃疡，十二指肠溃疡，非溃疡性消化不良和胃炎的关系和致病作用仍存在争议。作者研究了每天灌胃H的效果。盐水中的幽门螺杆菌悬浮液 (10(8) CFU/ml) 和盐水H的无细菌滤液。85只sprague-dawley大鼠 (体重150至200g) 具有正常粘膜和手术产生的实验性胃溃疡。具有先前存在的实验性胃溃疡的I组大鼠 (n = 30) 接受H。幽门螺杆菌悬液 (ATCC 43504，10(8) CFU/ml); 患有实验性胃溃疡的II组大鼠 (n = 20) 接受了无细菌H。pylori滤液; III组溃疡大鼠 (n = 20) 仅接受生理盐水；没有溃疡的第IV组对照大鼠 (n = 15) 接受完整的幽门螺杆菌生物悬浮液 (ATCC 43504，10(8) CFU/ml)。死亡时，用解剖显微镜测量溃疡表面积。获得全层切片的定量和定性组织学参数，包括剩余粘膜坏死的面积; 恢复的粘膜结构的特征和细胞组成; 以及是否存在炎症，包括嗜中性粒细胞和淋巴细胞的计数。使用常规，特殊和免疫组织化学染色在表面粘液和隐窝中鉴定幽门螺杆菌生物。我们的结果表明，在患有先前存在的胃溃疡的大鼠的胃中，完整的幽门螺杆菌生物或无细菌的幽门螺杆菌滤液的持续存在导致溃疡的延迟愈合和慢性活动性炎症的持续存在。然而，对具有完整胃粘膜的假手术大鼠每天施用幽门螺杆菌生物体的悬浮液，尽管在死亡时鉴定了表面幽门螺杆菌生物体，但没有导致溃疡或炎症。作者得出结论，单独的幽门螺杆菌对大鼠的完整胃粘膜几乎没有影响或没有影响，完整的生物或无细菌的滤液会导致具有活动性慢性炎症的预先存在的溃疡的类似延长和延迟愈合，并且导致胃粘膜完整性破坏的诱发因素的存在可能是幽门螺杆菌增强炎症和胃组织损伤所必需的。图像: 图1图2图3图4图5
发表日期： 1992 Oct 31
英文标题： Eradicating Helicobacter pylori in patients with duodenal ulcer: Authors' reply.[全文]
中文标题： 根除十二指肠溃疡患者的幽门螺杆菌: 作者的回复。
论文作者： Li, Arthur K C
英文摘要：
中文摘要：
发表日期： 1992 Oct 31
英文标题： Eradicating Helicobacter pylori in patients with duodenal ulcer.[全文]
中文标题： 根除十二指肠溃疡患者的幽门螺杆菌。
论文作者： Logan, R. P.; Baron, J. H.; Misiewicz, J. J.
英文摘要：
中文摘要：
发表日期： 1992 Oct 11
中文标题： 通过基于PCR的RAPD指纹图谱检测幽门螺杆菌临床分离株之间的DNA多样性。
论文作者： Akopyanz, N; Bukanov, N O; Westblom, T U; Kresovich, S; Berg, D E
英文摘要： The RAPD (or AP-PCR) DNA fingerprinting method was used to distinguish among clinical isolates of Helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. This method uses arbitrarily chosen oligonucleotides to prime DNA synthesis from genomic sites to which they are fortuitously matched, or almost matched. Most 10-nt primers with > or = 60% G + C yielded strain-specific arrays of up to 15 prominent fragments, as did most longer (> or = 17-nt) primers, whereas most 10-nt primers with 50% G+C did not. Each of 64 independent H. pylori isolates, 60 of which were from patients in the same hospital, was distinguishable with a single RAPD primer, which suggests a high level of DNA sequence diversity within this species. In contrast, isolates from initial and followup biopsies were indistinguishable in each of three cases tested.Images:
中文摘要： RAPD (或ap-pcr) DNA指纹图谱方法用于区分幽门螺杆菌的临床分离株，幽门螺杆菌是一种长期携带与胃炎，消化性溃疡和胃癌有关的细菌。该方法使用任意选择的寡核苷酸从它们偶然匹配或几乎匹配的基因组位点引发DNA合成。大多数具有> 或 = 60% G + C的10-nt引物产生多达15个显著片段的菌株特异性阵列，大多数更长 (> 或 = 17-nt) 的引物也是如此，而大多数具有50% G + C的10-nt引物没有。64个独立的幽门螺杆菌分离株中的每一个，其中60个来自同一家医院的患者，可以用单个RAPD引物区分，这表明该物种中DNA序列的多样性很高。相比之下，在测试的三个病例中，来自初始和随访活检的分离株均无法区分。图像:
发表日期： 1992 Oct
英文标题： Nonsteroidal anti-inflammatory drugs and ulcers.[全文]
中文标题： 非甾体类抗炎药和溃疡。
论文作者： Soll, A H
英文摘要：
中文摘要：
发表日期： 1992 Oct
中文标题： 应用聚合酶链反应特异性检测空肠弯曲菌和大肠弯曲菌。
论文作者： Oyofo, B A; Thornton, S A; Burr, D H; Trust, T J; Pavlovskis, O R; Guerry, P
英文摘要： Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C. coli and 47 strains of C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Vibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a nonradioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli DNA, or the equivalent of four bacteria. In stools seeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. The assay was also successfully used to detect C. coli in rectal swab specimens from experimentally infected rabbits and C. jejuni in human stool samples.Images:
中文摘要： 通过使用聚合酶链反应 (PCR) 开发了空肠弯曲杆菌和大肠弯曲杆菌在临床标本中的常规检测方法。来自C. coli.VC167的flaA基因的保守5' 区域的寡核苷酸引物对用于通过PCR扩增450-bp区域。该引物对特异性检测到4株大肠杆菌和47株空肠弯曲杆菌; 但未检测到胎儿弯曲杆菌，拉里弯曲杆菌，上唾液弯曲杆菌，嗜冷弯曲杆菌，butzleri弯曲杆菌，肠弯曲杆菌，直肠沃瑞氏杆菌，幽门螺杆菌，大肠杆菌，志贺氏菌。沙门氏菌属，霍乱弧菌，弗氏柠檬酸杆菌或气单胞菌。通过使用PCR产物内部的非放射性标记的探针，该测定可以检测少至0.0062 pg的纯化的大肠杆菌DNA，或四种细菌的等价物。在接种了大肠杆菌细胞的粪便中，探针每次PCR测定可以检测30至60个细菌。该分析也成功地用于检测实验感染兔子的直肠拭子标本中的大肠杆菌和人类粪便样本中的空肠弯曲杆菌。
发表日期： 1992 Oct
英文标题： Lipid A in Helicobacter pylori.[全文]
中文标题： 幽门螺杆菌中的脂质A。
论文作者： Mattsby-Baltzer, I; Mielniczuk, Z; Larsson, L; Lindgren, K; Goodwin, S
英文摘要： Free lipid A of Helicobacter pylori was characterized with regard to chemical composition, reactivity with anti-lipid A antibodies, and activity in a Limulus lysate assay. The predominant fatty acids of H. pylori lipid A were 3-OH-18:0, 18:0, 3-OH-16:0, 16:0, and 14:0. Hexosamine was present in amounts similar to those in Campylobacter jejuni or Salmonella typhimurium lipid A. The lipopolysaccharide of H. pylori contained 2-keto-3-deoxyoctonic acid, a common constituent of enterobacterial and C. jejuni lipopolysaccharides. In the enzyme-linked immunosorbent assay, the doses of lipid A required to inhibit anti-lipid A by 50% (EI50 values) by absorption of the immune (rabbit) serum were 7.9, 1.2, and 1.4 micrograms of O-deacylated lipid A's from H. pylori, C. jejuni, and S. typhimurium per ml, respectively. The lower reactivity of H. pylori lipid A compared with those of the other two lipid A preparations (as shown by the higher EI50 value) was underscored by the use of a murine monoclonal anti-lipid A antibody in the inhibition assay. An EI50 value was not obtained at the concentrations tested for H. pylori lipid A; the corresponding figures for C. jejuni and S. typhimurium lipid A's were 13 and 14 micrograms/ml, respectively. No inhibition was obtained with H. pylori lipopolysaccharide, which showed a low-molecular-weight profile on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The activity of H. pylori lipid A in the Limulus assay was approximately 71 and 650 times lower than those of C. jejuni and S. typhimurium lipid A's, respectively. These findings suggest that lipid A is an integral part of the outer cell wall of H. pylori. The lower reactivity of H. pylori lipid A with anti-lipid A antibodies and in the Limulus assay compared with that of C. jejuni or S. typhimurium lipid A may be explained by a different composition of the fatty acids, especially the 3-hydroxy fatty acids, and a possible deviating phosphorylation pattern.
中文摘要： 幽门螺杆菌的游离脂质A的特征在于化学组成，与抗脂质A抗体的反应性以及the裂解物测定中的活性。幽门螺杆菌脂质A的主要脂肪酸为3-OH-18:0、3-OH-16:0、16:0和14:0。己糖胺的含量与空肠弯曲杆菌或鼠伤寒沙门氏菌脂质A中的含量相似。幽门螺杆菌的脂多糖含有2-酮-3-脱氧辛酸，这是肠细菌和空肠弯曲杆菌脂多糖的常见成分。在酶联免疫吸附测定中，通过吸收免疫 (兔) 血清将抗脂质A抑制50% (EI50值) 所需的脂质A剂量为7.9，1.2和1.4微克O-脱酰基脂质A来自H。幽门螺旋杆菌，C.空肠和S.每毫升鼠伤寒。通过在抑制测定中使用鼠单克隆抗脂质A抗体，强调了幽门螺杆菌脂质A与其他两种脂质a制剂相比的较低反应性 (如由较高的EI50值所示)。在幽门螺杆菌脂质A的测试浓度下未获得EI50值; 空肠弯曲杆菌和鼠伤寒沙门氏菌脂质A的相应数字分别为13和14微克/ml。幽门螺杆菌脂多糖没有抑制作用，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上显示出低分子量分布。幽门螺杆菌脂质A在鲎试验中的活性分别比空肠弯曲杆菌和鼠伤寒沙门氏菌脂质A的活性低约71和650倍。这些发现表明脂质A是幽门螺杆菌外细胞壁的组成部分。与空肠弯曲杆菌或鼠伤寒沙门氏菌脂质A相比，幽门螺杆菌脂质A与抗脂质A抗体和鲎试验的反应性较低，这可以通过脂肪酸的不同组成来解释，尤其是3-羟基脂肪酸，以及可能的偏离磷酸化模式。
发表日期： 1992 Oct
中文标题： 胃b细胞粘膜相关淋巴组织淋巴瘤: 56例患者的临床病理研究。
论文作者： Castrillo, J M; Montalban, C; Obeso, G; Piris, M A; Rivas, M C
英文摘要： Clinico-pathological features of 56 patients with primary gastric lymphoma were evaluated retrospectively. All cases were regraded according to a classification of Isaacson et al into high grade and low grade B-cell mucosa associated lymphoid tissue lymphoma. A third group of mixed grade was recognised in 11 patients with low grade who also had occasional areas of high grade. Low grade and mixed grade patients had a 100% actuarial survival at 156 months, which was significantly better (p < 0.01) than that of 52% for patients with high grade disease. Different treatment methods--surgery, chemotherapy, or a combination of both--did not significantly affect survival. Low grade tumours occurred mainly in men with a history of several years, and who presented with non-specific gastric symptoms without remarkable exploratory or laboratory findings: most patients were in stage IE-IIE and achieved remission and cure. High grade can have a shorter history, systemic symptoms, abnormal exploratory and laboratory findings, gastric tumour masses, stage IV disease, and a worse outcome. The only significant prognostic factors for survival were the type of lymphoma and stage IV disease. These findings support the Isaacson classification system which separates two extreme groups of gastric lymphomas with different morphology, behaviour, and outcome. The presence of limited areas of high grade in a specimen showing low grade does not change the outcome but suggests that primary gastric lymphoma forms a continuum between these extreme types.
中文摘要： 回顾性评估了56例原发性胃淋巴瘤患者的临床病理特征。所有病例根据Isaacson等人的分类分为高级别和低级别b细胞粘膜相关淋巴组织淋巴瘤。在11例低年级患者中识别出第三组混合年级，这些患者偶尔也有高年级区域。低级别和混合级别患者在156个月时的精算生存率100%，明显优于高级别患者的52% (p <0.01)。不同的治疗方法-手术，化疗或两者结合-不会显着影响生存率。低度肿瘤主要发生在具有数年病史的男性中，并且表现出非特异性胃症状而没有明显的探索性或实验室检查结果: 大多数患者处于ie-iie期，并获得缓解和治愈。高等级可能具有较短的病史，全身症状，异常的探查和实验室检查结果，胃肿瘤肿块，IV期疾病和较差的结果。生存的唯一重要预后因素是淋巴瘤的类型和IV期疾病。这些发现支持Isaacson分类系统，该系统将具有不同形态，行为和结果的两个极端胃淋巴瘤组分开。在显示低级别的标本中存在有限的高级别区域不会改变结果，但表明原发性胃淋巴瘤在这些极端类型之间形成了连续体。
发表日期： 1992 Oct
英文标题： Changing pattern of gastric cancer in Oxfordshire.[全文]
中文标题： 牛津郡胃癌的变化模式。
论文作者： Rios-Castellanos, E; Sitas, F; Shepherd, N A; Jewell, D P
英文摘要： This study compares the incidence rates of histologically confirmed gastric carcinoma in Oxfordshire in two five year periods (1960-64, 1984-88). Data were available for 215 patients in the first period, and 200 in the second. The overall incidence fell from 18/100,000 to 15/100,000 but when analysed for site, the incidence of antral tumours fell from 10 to 4.5/100,000. In contrast, there was an increase from 2.8 to 5.2/100,000 of tumours of the cardia. These changes were more pronounced in men. There was a marked association between smoking and tumours of the cardia (relative risk 4.5). Helicobacter pylori was associated with 37.5% of tumours in the 1960s series compared with 25% in the later series. The changing patterns of incidence of gastric carcinoma may, in part, be related to changes in smoking habits and perhaps a change in incidence of H pylori infection.Images: Figure 2
中文摘要： 这项研究比较了牛津郡在两个五年期间经组织学证实的胃癌的发病率 (1960-64，1984-88)。第一阶段有215名患者的数据，第二阶段有200名患者的数据。总体发病率从18/100，000下降到15/100，000，但是当对部位进行分析时，肛门肿瘤的发病率从10/100,000下降到4.5/。相反，the门肿瘤的数量从2.8增加到5.2/100,000。这些变化在男性中更为明显。吸烟与贲门肿瘤之间存在显著关联 (相对危险度4.5)。在19 60年代系列中，幽门螺杆菌与肿瘤37.5% 有关，而在后来的系列中，幽门螺杆菌与25% 有关。胃癌发病率的变化模式可能部分与吸烟习惯的变化有关，也可能与幽门螺杆菌感染发病率的变化有关。图像: 图2
发表日期： 1992 Oct
中文标题： 十二指肠溃疡家族三代幽门螺杆菌克隆变异的证据。
论文作者： Nwokolo, C U; Bickley, J; Attard, A R; Owen, R J; Costas, M; Fraser, I A
英文摘要： Nine members of a family with a high incidence of duodenal ulcer disease were studied by interview, examination of hospital records, endoscopy, and antral biopsy. Helicobacter pylori was confirmed by CLO test, histology and culture. DNA extraction from pure isolates of H pylori was possible in six family members and strain typing was performed by restriction fragment length polymorphism. DNA restriction digestion was followed by vacublotting and then DNA hybridisation, using a cDNA probe complimentary to H pylori rRNA cistrons. Eight of the nine family members were H pylori positive by CLO test and histology. Five had duodenal ulcer disease. Three family members (one from each generation) harboured clonal variants of a single parent strain of H pylori but only two had duodenal disease. The other three members harboured different strains. Intrafamilial clustering of clonal variants of H pylori occurs in some duodenal ulcer disease families. Family members however, may develop duodenal disease irrespective of the colonising strain.Images: Figure 3Figure 4
中文摘要： 通过访谈，医院记录检查，内窥镜检查和胃窦活检研究了一个十二指肠溃疡病高发家庭的9名成员。幽门螺杆菌通过CLO试验，组织学和培养证实。在六个家庭成员中，可以从纯的幽门螺杆菌分离株中提取DNA，并通过限制性片段长度多态性进行菌株分型。DNA限制性消化后进行真空印迹，然后使用与幽门螺杆菌rRNA顺反子互补的cDNA探针进行DNA杂交。通过CLO测试和组织学检查，九个家庭成员中有八个是幽门螺杆菌阳性。五名患有十二指肠溃疡疾病。三个家庭成员 (每代一个) 拥有幽门螺杆菌单亲菌株的克隆变异，但只有两个患有十二指肠疾病。其他三个成员拥有不同的菌株。幽门螺杆菌克隆变异的家族内聚集发生在某些十二指肠溃疡疾病家族中。然而，家庭成员可能会发生十二指肠疾病，而与定植菌株无关。图像: 图3图4
发表日期： 1992 Oct
中文标题： 接受阿莫西林和替硝唑治疗的幽门螺杆菌阳性儿童的18个月随访。
论文作者： Oderda, G; Vaira, D; Ainley, C; Holton, J; Osborn, J; Altare, F; Ansaldi, N
英文摘要： Sixty three children with dyspepsia (mean age 12 years, range one to 18, M/F 41/22) were Helicobacter pylori positive by histology of gastric antral biopsy specimens and were treated with a six week course of amoxycillin (50 mg/kg) and tinidazole (20 mg/kg). The endoscopic diagnoses were: normal (16), nodular gastritis (19), oesophagitis (four), duodenal ulcer (13), and gastric ulcer (11). H pylori was eradicated in 54 (87%) and histological gastritis resolved in 51 and was improved in the other three. Repeat investigation was offered at six monthly intervals. Reinfection was found in three of 34 (9%) at six months, in none of 22 at 12 months, and in two of 18 (11%) at 18 months, yielding an 18 month cumulative relapse rate of 20%. Children with persisting infection despite treatment remained positive during follow up. Serum H pylori IgG concentrations fell after treatment (p < 0.001), and for individual children during follow up there was a progressive decline, but an increased concentration indicated recurrence. After eradication of H pylori by combined amoxycillin and tinidazole treatment, only a minority of children relapse during the ensuing 18 months.
中文摘要： 63名消化不良儿童 (平均年龄12岁，1至18岁，M/F 41/22) 通过胃窦活检标本的组织学检查呈幽门螺杆菌阳性，并接受了六周疗程的阿莫西林 (50 mg/kg) 和替硝唑 (20 mg/kg)。内镜诊断为: 正常 (16)，结节性胃炎 (19)，食管炎 (4)，十二指肠溃疡 (13) 和胃溃疡 (11)。幽门螺杆菌在54例 (87% 例) 中根除，组织学胃炎在51例中消退，在其他3例中有所改善。每隔六个月进行一次重复调查。在6个月的34个月中有3个 (9%)，在12个月的22个中没有发现再感染，在18个月的18个 (11%) 中有2个发现了再感染，18个月的累积复发率为20%。尽管治疗仍持续感染的儿童在随访期间保持阳性。治疗后血清幽门螺杆菌IgG浓度下降 (p <0.001)，在随访期间，个别儿童的血清幽门螺杆菌IgG浓度逐渐下降，但浓度升高表明复发。通过阿莫西林和替硝唑联合治疗根除幽门螺杆菌后，在随后的18个月中只有少数儿童复发。
发表日期： 1992 Nov-Dec
英文标题： Discussion: Current Clinical Management of Peptic Ulcer Disease.[全文]
中文标题： 讨论: 消化性溃疡疾病的当前临床管理。
论文作者： Berlin, Roger G.
英文摘要：
中文摘要：
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