2015年1月Pubmed幽门螺杆菌相关文献汇总

宋函憶

盛京医院

1.幽门螺杆菌感染与终末期肾病的关系：中国人群的回顾性对列研究

慢性肾病或心血管疾病患者伴幽门螺杆菌感染时，其终末期肾病的风险增加

Association between Helicobacter pylori infection and the subsequent risk of end-stage renal disease: a nationwide population-based cohort study.

Lin SY(1), Lin CL, Liu JH, Yang YF, Huang CC, Kao CH.

Int J Clin Pract. 2015 Jan 21. doi: 10.1111/ijcp.12602.

[Epub ahead of print]

Abstract

BACKGROUND & AIMS: The association between Helicobacter pylori

infection and end-stage renal disease (ESRD) events remains unknown. We assessed the

relationship between H. pylori infection requiring hospital admission and the subsequent risks of ESRD.

METHODS: This was a retrospective cohort study in which

data from the National Health Insurance system of Taiwan

was used. The H. pylori-infected cohort comprised 20,068 patients. Each participant was frequency-matched by age and sex with 4 individuals from the general population without H. pylori-infected. Cox

proportional hazards regression analysis was used to estimate

the influence of H. pylori infection on the risk of ESRD.

RESULTS: The overall incidence of ESRD was 3.72 times greater

in the H. pylori-infected cohort than in the non-infected cohort (11.1 vs. 2.96 per 1000

person-years), with an adjusted HR of 2.58 [95% confidence interval

(CI) = 2.33-2.86]. The risk of ESRD markedly increased in patients with H. pylori

infection combined with at least one of the following concomitant

comorbidities: hypertension, diabetes, hyperlipidaemia and coronary artery disease.

CONCLUSIONS: This is currently the largest nation-based study in which the risk of ESRD in H. pylori-infected patients was examined. H. pylori infection was

associated with a subsequent risk of ESRD. H. pylori-infected patients with

concomitant chronic kidney disease (CKD) or cardiovascular

disease (CVD) risk factors were at higher risk of ESRD than were those who had a single CKD or CVD

risk factor.

2.亚洲地区幽门螺杆菌感染与胃食管反流性疾病关系的Meta分析

Meta分析显示：幽门螺杆菌的根除治疗增加亚洲地区胃食管反流性疾病的风险，

但在亚洲国家，幽门螺杆菌感染在胃食管反流患者中仍然一个是不确定和重要的问题。

Helicobacter pylori Infection in Gastroesophageal Reflux Disease in the Asian Countries.

Hong SJ(1), Kim SW(2).Gastroenterol Res Pract. 2015;2015:985249.

doi: 10.1155/2015/985249. Epub 2015 Jan 6.

Abstract

Helicobacter pylori infection, a common infection in many countries,

is related to the clinical course of upper gastrointestinal diseases. Gastroesophagealreflux disease (GERD) is a common esophageal

disease in Western countries and its

prevalence is increasing in Asian countries. The pathophysiology

of GERD is multifactorial. Although no single factor has been

isolated as the cause of GERD,a negative association between

the prevalence of H. pylori and the severity of

GERD, including Barrett's esophagus, has been demonstrated

in epidemiological studies. The high prevalence of H. pylori

infection affects the incidence of GERD in Asian countries.

In the subjects with East Asian CagA-positive strains, acid

injury may be minimized by hypochlorhydria from pangastritis

and gastric atrophy.Additionally, host genetic factors may affect

the development of GERD. The interactions between genetic factors

and the virulence of H. pylori infection may be the reason for the low

prevalence of GERD in Asian countries. H. pylori eradication is not

considered pivotal in GERD exacerbation based on

evidence from Western studies. A recent meta-analysis demonstrated that eradication therapy of

H. pylori was related to a higher risk of developing

de novo GERD in Asian studies. H. pylori infection remains

an inconclusive and important issue in GERD in Asian countries.

PMCID: PMC4302361

PMID: 25642246 [PubMed]

3. 幽门螺杆菌感染与心肌梗死关系的Meta分析

Helicobacter pylori and the Risk of Myocardial Infarction: Any Missing Data

in the Meta-Analysis?

Pellicano R(1).Helicobacter. 2015 Jan 30.

doi: 10.1111/hel.12212. [Epub ahead of print]

PMID: 25640774 [PubMed - as supplied by publisher]

4. 使用多重基因分析系统（MGAS）定性和定量的检测幽门螺杆菌

MGAS在幽门螺杆菌检测中的敏感性和特异性为92.9 和 92.4%

A Creative Helicobacter pylori Diagnosis Scheme Based on Multiple Genetic Analysis System:

Qualification and Quantitation.

Zhou L(1), Zhao F, Hu B, Fang Y, Miao Y, Huang Y, Ji D,

Zhang J, Xu L, Zhang Y,Bao Z, Zhao H.Helicobacter. 2015 Jan 29.

doi: 10.1111/hel.12206. [Epub ahead of print]

Abstract

BACKGROUND: Currently, several diagnostic assays for

Helicobacter pylori (H. pylori) are available, but each has

some limitations.Further, a high-flux quantitative assay is required

to assist clinical diagnosis and monitor the

effectiveness of therapy and novel vaccine candidates.

METHODS: Three hundred and eighty-seven adult patients [nonulcer dyspepsia (NUD) 295,peptic ulcer disease (PUD)

77, gastric cancer (GC) 15] were enrolled for

gastrointestinal endoscopies. Three biopsy samples from

gastric antrum were collected for the following tests:

culture, rapid urease test (RUT),histopathology, conventional

polymerase chain reaction (PCR), and Multiple

Genetic Analysis System (MGAS). The diagnostic capability

of H. pylori for all methods was evaluated through the

receiver operating characteristic (ROC) curves.

RESULTS: Based on the gold standard, the sensitivity and

specificity of MGAS were 92.9 and 92.4%, and positive predict

value (PPV) and negative predict value (NPV)

were 96.0 and 87.1%, respectively. All the above parameters

of MGAS were higher than that of culture (except its specificity),

RUT and histopathology, and nearly closed to that of conventional

PCR.The area under curve (AUC) was 0.7575

(Culture), 0.8870 (RUT), 0.9000 (Histopathology), 0.9496 (Conventional PCR), and

0.9277 (MGAS). No significant statistical difference was observed for the

H. pylori DNA load in different disease groups (p = .067). In contrast, a

statistically significant difference in the H. pylori DNA copy number was

observed based on age (p = .043) and gender (p = .021).

CONCLUSIONS: The data showed that MGAS performed well in detecting H. pylori

infection. Furthermore, the quantitative analysis showed that the load of

H. pylori was significantly different within both age and gender groups. These

results suggested that MGAS could be a potential alternative method for clinical

detection and monitoring of the effectiveness of H. pylori therapy.

PMID: 25640660 [PubMed - as supplied by publisher]

5. 韩国C13尿素呼气试验（不含柠檬酸、高剂量）在幽门螺杆菌根除治疗后的准确性研究

目前在韩国所使用的不含柠檬酸的C13尿素呼气试验的临界值特异性差，导致假阳性。

The Diagnostic Validity of Citric Acid-Free, High Dose <sup>13</sup> C-Urea Breath Test AfterHelicobacter pylori Eradication in Korea.

Kwon YH(1), Kim N, Lee JY, Choi YJ, Yoon K, Hwang JJ, Lee HJ,

Lee A, Jeong YS, Oh S, Yoon H, Shin CM, Park YS, Lee DH.Helicobacter.

2015 Jan 29. doi: 10.1111/hel.12189. [Epub ahead of print]

Abstract

BACKGROUND: The (13) C-urea breath test ((13) C-UBT) is a noninvasive method for diagnosing Helicobacter pylori (H. pylori) infection. The aims of this study wereto evaluate the diagnostic validity of the (13) C-UBT cutoff value and to identify influencing clinical factors responsible for aberrant results.

METHODS: (13) C-UBT (UBiTkit; Otsuka Pharmaceutical, cutoff value: 2.5‰) results

in the range 2.0‰ to 10.0‰ after H. pylori eradication therapy were compared with the results of endoscopic biopsy results of the antrum and body. Factors

considered to affect test results adversely were analyzed.

RESULTS: Among patients with a positive (13) C-UBT result (2.5‰ to 10.0‰,

n = 223) or a negative (13) C-UBT result (2.0‰ to < 2.5‰, n = 66) after H. pylori

eradication, 73 patients (34.0%) were false positive, and one (1.5%) was false

negative as determined by endoscopic biopsy. The sensitivity, specificity,

false-positive rate, and false-negative rate for a cutoff value of 2.5‰ were

99.3%, 47.1%, 52.9%, and 0.7%, respectively, and positive and negative predictive

values of the (13) C-UBT were 67.3% and 98.5%, respectively. Multivariate

analysis showed that a history of two or more previous H. pylori eradication

therapies (OR = 2.455, 95%CI = 1.299-4.641) and moderate to severe gastric

intestinal metaplasia (OR = 3.359, 95%CI = 1.572-7.178) were associated with a

false-positive (13) C-UBT result.

CONCLUSION: The (13) C-UBT cutoff value currently used has poor specificity for

confirming H. pylori status after eradication, and this lack of specificity is

exacerbated in patients that have undergone multiple prior eradication therapies

and in patients with moderate to severe gastric intestinal metaplasia. In

addition, the citric-free (13) C-UBT would increase a false-positive (13) C-UBT

result.

PMID: 25640474 [PubMed - as supplied by publisher]

6. 幽门螺杆菌的分子致病机理

综述文章：从幽门螺杆菌黏附、入侵胃黏膜上皮细胞、细胞内炎症通路的激活、到表观遗传修饰来进行阐述。

Molecular Mechanisms of Helicobacter Pylori Pathogenesis.

De Falco M(1), Lucariello A, Iaquinto S, Esposito V, Guerra G, De Luca A.J Cell Physiol. 2015 Jan 29. doi: 10.1002/jcp.24933. [Epub ahead of print]

Abstract

Helicobacter pylori infects 50% of mankind. The vast majority of H. pylori

infection occurs in the developing countries where up to 80% of the middle-aged

adults may be infected. Bacterial infection causes an inflammatory response that

proceeds through a series of intermediated stages of precancerous lesions

(gastritis, atrophy, intestinal metaplasia and dysplasia). Among infected

individuals, approximately 10% develops severe gastric lesions such as peptic

ulcer disease, 1-3% progresses to gastric cancer (GC) with a low 5 year survival

rate, and 0.1% develops mucosa-associated lymphoid tissue (MALT). GC is one of

the most common cancer and the third leading cause of cancer-related deaths

worldwide. In this review we have summarized the most recent papers about

molecular mechanisms of Helicobacter pylori pathogenesis. The main important

steps of H. pylori infection such as adhesion, entry in epithelial gastric cells,

activation of intracellular pathways until epigenetic modifications have been

described. This article is protected by copyright. All rights reserved.

PMID: 25639461 [PubMed - as supplied by publisher]

7. 新加坡研究，幽门螺杆菌一线治疗方案的比较：标准三联方案vs序贯疗法vs伴同疗法（疗程10天）

ITT方案分析时，三种方案的根除率均大于80%；MITT和PP方案分析时，三种方案的根除率均大于90%。抗生素双重耐药和依从性差可以预测根除治疗的失败。

Ten-day triple therapy versus sequential therapy versus concomitant therapy as first line treatment for Helicobacter pylori infection.

Ang TL(1), Fock KM, Song M, Ang D, Kwek AB, Ong J, Tan J, Teo EK, Dhamodaran S.J Gastroenterol Hepatol. 2015 Jan 16. doi: 10.1111/jgh.12892. [Epub ahead of

print]

Abstract

BACKGROUND AND AIM: Clarithromycin-based triple therapy (TT) is the first line

treatment for H. pylori infection in Singapore. There is awareness that TT may no

longer be effective due to increased clarithromycin resistance rates. Sequential

therapy (ST) and concomitant therapy (CT) are alternative treatment regimens. The

study aimed to compare the efficacy of 10-day TT, ST and CT as first line

treatment for H. pylori infection.

METHODS: A randomized study conducted in a teaching hospital. Patients aged 21

years and older with newly diagnosed H. pylori infection were randomized to

10-day TT, ST or CT. Treatment outcome was assessed by 13-carbon urea breath test at least 4 weeks after therapy. Intention to treat (ITT), modified ITT (MITT) and

per protocol (PP) analysis of the eradication rates were performed.

RESULTS: A total of 462 patients were enrolled (ST: 154; TT 155; CT 153). Patient

demographics were similar. Eradication rates for ST vs. TT vs. CT: ITT analysis:

84.4% vs. 83.2% vs. 81.7% (p= NS); MITT analysis: 90.3% vs. 92.1% vs. 94.7% (p =

NS); PP analysis: 94.1% vs. 92.8% vs. 95.4% (p = NS). Antibiotic resistance rates

for amoxicillin, clarithromycin and metronidazole were 4.7%, 17.9% and 48.1%

respectively. Dual clarithromycin and metronidazole resistance occurred in 7.5%.

Dual resistance and lack of compliance were predictors of treatment failure.

CONCLUSIONS: TT, ST and CT all achieved eradication rates above 80% on ITT and above 90% on MITT and PP analyses. Dual resistance and lack of compliance were predictors of treatment failure. ClinicalTrials.gov: NCT02092506.

PMID: 25639278 [PubMed - as supplied by publisher]

8.儿童幽门螺杆菌感染三联疗法根除方案中雷贝拉唑的剂量

儿童（≥1岁，<16岁）推荐按公斤体重计算（每日两次）：

6-10公斤：10毫克；

10-30公斤：15毫克；

大于等于30公斤：20毫克

Helicobacter pylori Treatment in Children: Defining a Dose for Rabeprazole as a Part of a Triple Therapy Regimen.

Kimko H(1), Thyssen A, Mould DR, Mannaert E, Treem WR. J Clin Pharmacol. 2015 Jan 5. doi: 10.1002/jcph.457. [Epub ahead of print]

Abstract

The proton pump inhibitor, rabeprazole, has been studied in children for the

treatment of gastroesophageal reflux disease (GERD). In adults, rabeprazole is

indicated for Helicobacter pylori eradication in combination with amoxicillin and

clarithromycin. Nonlinear mixed effects modeling was conducted to estimate

pharmacokinetic (PK) parameters for rabeprazole and its thioether metabolite from

336 subjects, 35% of whom were children 1-11 years with GERD from phase I and III

studies. A 2-compartment disposition model with a transit absorption model

provided the best fit for rabeprazole PK. The steady-state area under the

concentration-time curves given several candidate doses were simulated to

identify a dose per each body weight group that is comparable to a 20 mg

twice-daily dose in adults, which is the recommended dose for treatment of H.

pylori in adults. Simulations provided the following recommended twice-daily

weight-based doses for children ≥1 year and <16 years: 10 mg for 6-10 kg, 15 mg for 10-30 kg, and 20 mg for ≥30 kg.

PMID: 25639255 [PubMed - as supplied by publisher]

9. Satureja bachtiarica Bung植物精油的抗幽门螺杆菌作用，以及其化学成分

Satureja bachtiarica Bung植物精油的主要成分为香芹酚（45.5％）和麝香草酚（27.9％），其在体外对幽门螺杆菌具有一定的抗菌活性，还有待于进一步的研究和探索。

Chemical composition and anti-Helicobacter pylori effect of Satureja bachtiarica Bunge essential oil.

Falsafi T(1), Moradi P(1), Mahboubi M(2), Rahimi E(3), Momtaz H(4), Hamedi B(5).Phytomedicine. 2015 Jan 15;22(1):173-7. doi: 10.1016/j.phymed.2014.11.012. Epub2014 Nov 26.

Abstract

Resistance of H. pylori strains to common antibiotics has been developed in

different parts of the world and continues to increase. It is important to

investigate the novel and efficient anti-H. pylori drugs, among which the plants

would be suitable sources. Satureja bachtiarica Bunge is traditionally used as

antimicrobial agent. In this study, we evaluated the antibacterial activity of S.

bachtiarica Bunge essential oil against 10 clinical isolates of Helicobacter

pylori by disc diffusion and agar dilution methods. The chemical composition of

essential oil was analyzed by GC and GC-MS. Carvacrol (45.5%) and thymol (27.9%)

were the primary constituents of oil, followed by p-cymene (4.4%), and

γ-terpinene (4.0%). S. bachtiarica essential oil showed strong antibacterial

activity against clinical isolates of H. pylori (17.6 ± 1.1 mm and

0.035 ± 0.13 μl/ml). Carvacrol, as the first main component, had a significant

role in this effect, whereas in the presence of thymol, the antibacterial effect

of carvacrol was reduced. Therefore, S. bachtiarica essential oil can be applied

as an alternative agent for treatment of H. pylori infections. More studies would

be required to better clarify its mechanism of action on H. pylori.

PMID: 25636887 [PubMed - in process]

10. 评估Exalenz Bioscience公司的13C尿素呼气试验产品BreathID(®)

Evaluation of Exalenz Bioscience's Breath ID for Helicobacter pylori detection.

Broide E(1), Shirin H.Expert Rev Mol Diagn. 2015 Jan 29:1-14. [Epub ahead of print]

Abstract

Carbon-labeled urea breath tests, which have high sensitivity and specificity,

are the preferred method used in epidemiological studies, screening dyspeptic

patients and assessing eradication or recurrence of Helicobacter pylori

infection. The principle of the (13)C-urea breath test relies upon the ability of

the H. pylori urease to hydrolyze the orally administered (13)C-urea. The

BreathID(®) (Exalenz Bioscience Inc., Union, NJ, USA) provides a competitive

solution for breath testing, including unique features such as automatic

continuous breath collection and analysis. This is an unattended convenient test,

with no human error as the correct part of the breath is collected and patients'

assistance is not required. The test results are available in real time at the

point of care and enable shortened breath testing procedures. Additionally,

several studies showing expanded utility of the BreathID in pediatrics, after

therapy and during proton pump inhibitors intake, further support the safety and

performance of the BreathID in the diagnosis of H. pylori.

PMID: 25634297 [PubMed - as supplied by publisher]

11.日本患者幽门螺杆菌感染和非甾体抗炎药引起消化性溃疡出血的时间变化趋势

在过去10年里，日本患者消化性溃疡出血的主要原因已不再是幽门螺杆菌感染，而是非甾体抗炎药的使用

Time Trends of the Impact of Helicobacter pylori Infection and Nonsteroidal Anti-Inflammatory Drugs on Peptic Ulcer Bleeding in Japanese Patients.

Nagasue T(1), Nakamura S, Kochi S, Kurahara K, Yaita H, Kawasaki K, Fuchigami T.Digestion. 2015;91(1):37-41. doi: 10.1159/000368810. Epub 2015 Jan 20.

Abstract

BACKGROUND/AIMS: Helicobacter pylori infection and the use of nonsteroidal

anti-inflammatory drugs (NSAIDs) are the main causes of peptic ulcers. The

purpose of the present study was to elucidate the time trends of the impact of H.

pylori infection and use of NSAIDs and/or antithrombotic agents on peptic ulcer

bleeding (PUB) in Japanese patients.

METHODS: We retrospectively reviewed 719 patients who had received endoscopic

hemostasis for PUB between 2002 and 2013. Subjects were divided into either the

first-half group (2002-2007, n = 363) or the second-half group (2008-2013, n =

356). The clinical characteristics of the patients, including the prevalence of

H. pylori infection and use of NSAIDs and antithrombotic agents, were compared

between the two groups.

RESULTS: Compared to the first-half group, patients in the second-half group were

characterized by older age (proportion of the patients above 60 years old, 63.9

vs. 76.7%, p = 0.0002), less frequent H. pylori infection (71.6 vs. 57.9%, p <

0.001) and more frequent NSAID intake (39.9 vs. 48.6%, p = 0.02). No significant

difference was observed regarding the use of antithrombotic agents between the

two groups (18.6 vs. 23.3%, p = 0.13). The prevalence of H. pylori infection and

proportion of patients above 60 years old were significantly different between

the two groups in a multivariate analysis.

CONCLUSION: The main cause of PUB has clearly shifted from H. pylori infection to

the use of NSAIDs over the last decade. © 2015 S. Karger AG, Basel.

PMID: 25632915 [PubMed - in process]

12.幽门螺杆菌感染的粪抗原检测的临床应用指南和成本效益

综述文章

Stool Antigen Tests for <i>Helicobacter pylori</i> Infection: A Review of Clinical and Cost-Effectiveness and Guidelines [Internet].

Ottawa (ON): Canadian Agency for Drugs and Technologies in Health; 2015 Jan.

CADTH Rapid Response Reports.

Abstract

There are two types of stool antigen tests for the diagnosis of H. pylori

infection, one based on enzyme immunoassay (EIA) and the other based on

immunochromatography (ICA). Both types of tests can be operated using either

monoclonal antibody or polyclonal antibodies. Although both are highly sensitive

and specific, the EIA-based tests appears to be more accurate than the ICA-based

tests. However, the ICA-based tests do not required specialized equipment, are

easy to use, and are useful for rapid diagnosis of H. pylori infection. The aim

of this report is to review the diagnostic accuracy, clinical effectiveness,

cost-effectiveness, and guidelines of stool antigen tests for H. pylori

infection.

PMID: 25632493 [PubMed]

13. 幽门螺杆菌感染是否增加冠心病的风险

Helicobacter pylori: Does it add to risk of coronary artery disease.

Sharma V(1), Aggarwal A(1).World J Cardiol. 2015 Jan 26;7(1):19-25. doi: 10.4330/wjc.v7.i1.19.

Abstract

Helicobacter pylori (H. pylori) is a known pathogen implicated in genesis of

gastritis, peptic ulcer disease, gastric carcinoma and gastric lymphoma. Beyond

the stomach, the organism has also been implicated in the causation of immune

thrombocytopenia and iron deficiency anemia. Although an area of active clinical

research, the role of this gram negative organism in causation of atherosclerosis

and coronary artery disease (CAD) remains enigmatic. CAD is a multifactorial

disease which results from the atherosclerosis involving coronary arteries. The

major risk factors include age, diabetes mellitus, smoking, hypertension and

dyslipidemia. The risk of CAD is believed to increase with chronic inflammation.

Various organisms like Chlamydia and Helicobacter have been suspected to have a

role in genesis of atherosclerosis via causation of chronic inflammation. This

paper focuses on available evidence to ascertain if the role of H. pylori in CAD

causation has been proven beyond doubt and if eradication may reduce the risk of

CAD or improve outcomes in these patients.

PMCID: PMC4306202 PMID: 25632315 [PubMed]

14.幽门螺杆菌尿素呼气试验准确性的Meta分析，对于消化不良患者采用UBT检测幽门螺杆菌的准确性高。

Accuracy of urea breath test in Helicobacter pylori infection: Meta-analysis.

Ferwana M(1), Abdulmajeed I(1), Alhajiahmed A(1), Madani W(1), Firwana B(1),

Hasan R(1), Altayar O(1), Limburg PJ(1), Murad MH(1), Knawy B(1). World J Gastroenterol. 2015 Jan 28;21(4):1305-14. doi: 10.3748/wjg.v21.i4.1305.

Abstract

AIM: To quantitatively summarize and appraise the available evidence of urea

breath test (UBT) use to diagnose Helicobacter pylori (H. pylori) infection in

patients with dyspepsia and provide pooled diagnostic accuracy measures.

METHODS: We searched MEDLINE, EMBASE, Cochrane library and other databases for studies addressing the value of UBT in the diagnosis of H. pylori infection. We included cross-sectional studies that evaluated the diagnostic accuracy of UBT in adult patients with dyspeptic symptoms. Risk of bias was assessed using QUADAS (Quality Assessment of Diagnostic Accuracy Studies)-2 tool. Diagnostic accuracy measures were pooled using the random-effects model. Subgroup analysis was conducted by UBT type ((13)C vs (14)C) and by measurement technique (Infrared spectrometry vs Isotope Ratio Mass Spectrometry).

RESULTS: Out of 1380 studies identified, only 23 met the eligibility criteria.

Fourteen studies (61%) evaluated (13)C UBT and 9 studies (39%) evaluated (14)C

UBT. There was significant variation in the type of reference standard tests used

across studies.Pooled sensitivity was 0.96 (95%CI: 0.95-0.97) andpooled

specificity was 0.93 (95%CI: 0.91-0.94). Likelihood ratio for a positive test was

12 and for a negative test was 0.05 with an area under thecurve of 0.985.

Meta-analyses were associated with a significant statistical heterogeneity that

remained unexplained after subgroup analysis. The included studies had a moderate

risk of bias.

CONCLUSION: UBT has high diagnostic accuracy for detecting H. pylori infection in

patients with dyspepsia. The reliability of diagnostic meta-analytic estimates

however is limited by significant heterogeneity.

PMCID: PMC4306177

PMID: 25632206 [PubMed - in process]

15. 也门症状儿童幽门螺杆菌和寄生虫的感染率

Prevalence of Helicobacter pylori and parasites in symptomatic children examined for

Helicobacter pylori antibodies, antigens, and parasites in Yemen.

Al-Mendalawi MD(1), Bin Mohanna MA.Saudi Med J. 2015 Jan;36(1):126-127. doi: 10.15537/smj.2015.1.10871.

[No abstract available].

PMID: 25630018 [PubMed - as supplied by publisher]

16. 多中心胃黏膜活检组织的不同转运条件对幽门螺杆菌分离培养的影响

比较不同的转运时间：5, 24和48小时以及不同的转运温度：4.67-29.14°C对幽门螺杆菌分离培养的影响。结论：胃黏膜活检组织标本的转运时间应该在取样后24小时内，温度应该控制在24°C以下。

Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences

Gong YN(1), Li YM(1), Yang NM(1), Li HZ(1), Guo F(1), Lin L(1), Wang QY(1), Zhang

JK(1), Ji ZZ(1), Mao JB(1), Mao JL(1), Shi ZC(1), Tang WH(1), Zhu XJ(1), Shao

W(1), Zhang XF(1), Wang XH(1), Tong YF(1), Jiang MZ(1), Chen GL(1), Wang ZY(1),

Tu HM(1), Jiang GF(1), Wu JS(1), Chen XP(1), Ding QL(1), Ouyang H(1), Jin FZ(1),

Xu YL(1), Zhang JZ(1).World J Gastroenterol. 2015 Jan 21;21(3):944-52. doi: 10.3748/wjg.v21.i3.944.

Abstract

AIM: To evaluate the efficacy of centralized culture and possible influencing

factors.

METHODS: From January 2010 to July 2012, 66452 patients with suspected

Helicobacter pylori (H. pylori) infection from 26 hospitals in Zhejiang and

Jiangsu Provinces in China underwent gastrointestinal endoscopy. Gastric mucosal

biopsies were taken from the antrum for culture. These biopsies were transported

under natural environmental temperature to the central laboratory in Hangzhou

city and divided into three groups based on their transport time: 5, 24 and 48 h.

The culture results were reported after 72 h and the positive culture rates were

analyzed by a χ (2) test. An additional 5736 biopsies from H. pylori-positive

patients (5646 rapid urease test-positive and 90 (14)C-urease breath

test-positive) were also cultured for quality control in the central laboratory

setting.

RESULTS: The positive culture rate was 31.66% (21036/66452) for the patient

samples and 71.72% (4114/5736) for the H. pylori-positive quality control

specimens. In the 5 h transport group, the positive culture rate was 30.99%

(3865/12471), and 32.84% (14960/45553) in the 24 h transport group. In contrast,

the positive culture rate declined significantly in the 48 h transport group

(26.25%; P < 0.001). During transportation, the average natural temperature

increased from 4.67 to 29.14 °C, while the positive culture rate declined from

36.67% (1462/3987) to 24.12% (1799/7459). When the temperature exceeded 24 °C,the positive culture rate decreased significantly, especially in the 48 h

transport group (23.17%).

CONCLUSION: Transportation of specimens within 24 h and below 24°C is reasonable and acceptable for centralized culture of multicenter H. pylori samples.

PMCID: PMC4299348

PMID: 25624729 [PubMed - in process]

17. 幽门螺杆菌感染可能参与引起非酒精性脂肪肝的肝细胞气球样变性，其可能是导致非酒精性脂肪肝（NAFL）向非酒精性脂肪性肝炎（NASH）转变的另一影响因素，根除幽门螺杆菌治疗有可能可以作为对NASH治疗的一个手段。

Helicobacter pylori infection might have a potential role in hepatocyte ballooning in nonalcoholic fatty liver disease.

Sumida Y(1), Kanemasa K, Imai S, Mori K, Tanaka S, Shimokobe H, Kitamura Y,

Fukumoto K, Kakutani A, Ohno T, Taketani H, Seko Y, Ishiba H, Hara T, Okajima A,

Yamaguchi K, Moriguchi M, Mitsuyoshi H, Yasui K, Minami M, Itoh Y.J Gastroenterol. 2015 Jan 28. [Epub ahead of print]

Abstract

BACKGROUND: Clinical data regarding Helicobacter pylori (H. pylori) infection in

nonalcoholic fatty liver disease (NAFLD) are limited. The aim was to evaluate H.

pylori infection in patients with NAFLD and its association with disease

severity.

METHODS: One hundred and thirty patients with biopsy-proven NAFLD [43 with

nonalcoholic fatty liver (NAFL) and 87 with nonalcoholic steatohepatitis (NASH)]

were recruited for blood samples for anti-H. pylori immunoglobulin G (IgG) and

standard biochemical tests were obtained after overnight fasting. Glucose

tolerance was evaluated by 75-g oral glucose tolerance test. Liver biopsies were

scored for NAFLD activity score (NAS), fibrosis and iron deposits.

RESULTS: H. pylori IgG seropositivity was found in 40 % of patients overall. The

prevalence of NASH was significantly higher in the patients with H. pylori IgG

seropositivity (81 %) than in those without (58 %, p = 0.008). Glucose

intolerance was similar between the two groups. The total NAS and the grade of

hepatocyte ballooning were higher in the patients with H. pylori IgG

seropositivity than in those without, while the hepatic iron grade was lower in

the patients with H. pylori IgG seropositivity than in those without. H. pylori

infection (p = 0.030), female gender (p = 0.029), and NAFIC score ≥ 2 points

(p < 0.001) could independently predict NASH in logistic regression analysis,

independent of age, obesity and glucose tolerance.

CONCLUSION: The association of H. pylori seropositivity with hepatocyte

ballooning suggests that H. pylori infection may represent another contributing

factor in the progression from NAFL to NASH. Eradicating H. pylori infection may

have therapeutic prospects in NASH treatment.

PMID: 25622927 [PubMed - as supplied by publisher]

18.幽门螺杆菌的CagL蛋白的RGDLXXL基序参与其与不同细胞系αVβ6黏合素的结合

Adhesion of Several Cell Lines to Helicobacter pylori CagL is Mediated by Integrin αVβ6 via an RGDLXXL Motif.

Barden S(1), Niemann HH(2).J Mol Biol. 2015 Jan 21. pii: S0022-2836(15)00027-3. doi:10.1016/j.jmb.2015.01.006. [Epub ahead of print]

Abstract

The Helicobacter pylori type IV secretion system pilus protein CagL mediates

interaction with host cells via its RGD motif. Here we analyzed prerequisites for

this interaction within CagL and on host cells. Various human cell lines were

tested for adhesion to CagL. HT-29 and 23132/87 cells adhered to immobilized

recombinant CagL in an RGD-dependent manner, while 293T (HEK) and A549 cells did not. In a competitive ELISA CagL competed with fibronectin for binding to the

ectodomains of integrins αVβ6 and αVβ8 but not αVβ1, αVβ3, αVβ5 and α5β1.Integrin αVβ6 acts as receptor for several viruses exposing an RGDLXXL motif.CagL also contains an RGDLXXL sequence. We individually mutated Leu79 and Leu82 of this motif to threonine, although both leucines are buried in the hydrophobic

core. Surprisingly, the ability of CagL variants L79T and L82T to support

adhesion was significantly reduced for 23132/87 cells and lost for MKN-45 and

HT-29 cells. The role of integrin αVβ6 in adhesion to CagL was investigated using

SW480 cells transfected with the integrin β6 subunit (SW480β6). These cells

adhered to CagL in an RGD-dependent manner, while mock-transfected SW480 cells

did not. The antibody 3G9 that blocks the function of integrin αVβ6 inhibited

adhesion of SW480β6, MKN-45, 23132/87 and HT-29 cells to CagL. In summary, CagLfeatures an RGDLXXL motif facilitating adhesion of several human cell lines via

integrin αVβ6. The buried location of Leu79 and Leu82 supports our previously

published hypothesis that CagL partly unfolds upon integrin binding.

PMID: 25617764 [PubMed - as supplied by publisher]

19. 儿童新诊断免疫性血小板减少症与幽门螺杆菌感染的相关性研究

幽门螺杆菌感染可能不是ITP患儿发病的一个主要致病因素；是否治疗幽门螺杆菌并不影响儿童急性ITP的治疗效果。

Association between Helicobacter pylori infection and newly diagnosed childhood immune thrombocytopenia.

g YY(1), Xiong H, Xu ZL, Li JX, Li H, Cai W, Han J, Li JJ, Guo L.Zhongguo Dang Dai Er Ke Za Zhi. 2015 Jan;17(1):22-5.

Abstract

OBJECTIVE: To study the role of Helicobacter pylori (H. pylori) infection in

newly diagnosed childhood immune thrombocytopenia (ITP).

METHODS: A total of 495 children with newly diagnosed ITP who were hospitalized

for the first time between January 2011 and December 2013 were included as the

case group. A total of 123 children with common respiratory tract infection (not

ITP or other diseases of blood system) were randomly selected as the control

group. All patients were divided into four groups by age: <1 year group, 1-3

years group, 3-7 years group, and 7-14 years group. The incidence of H. pylori

infection in all age groups and the clinical outcomes of ITP children with or

without H. pylori infection were retrospectively analyzed.

RESULTS: The incidence rate of H. pylori infection in the case group increased

with increasing age. There was no significant difference in the incidence rate of

H. pylori infection between the case and the control groups among subjects of the

same age (P>0.05). All the ITP patients were not given anti-H. pylori treatment

and only received the treatment (glucocorticoid and/or immunoglobulin) for ITP,

and their remission rate declined with increasing age. There was no significant

difference in the remission rate between the ITP children with H. pylori

infection and those without H. pylori infection in the same age group (P>0.05).

CONCLUSIONS: H. pylori infection may not be a major cause of ITP in children, and

the clinical outcomes of children with acute ITP are not affected by receiving

anti-H. pylori treatment or not.

PMID: 25616287 [PubMed - in process]

20.利用剑桥学院Optum Labs的数据分析美国人群幽门螺杆菌诊断检测试剂的订购模式

Use of the Optum Labs Data Warehouse to Assess Test Ordering Patterns for Diagnosis of Helicobacter pylori Infection in the United States.

Theel ES(1), Johnson RD(2), Plumhoff E(3), Hanson CA(4).J Clin Microbiol. 2015 Jan 21. pii: JCM.03464-14. [Epub ahead of print]